Genetic Diversity of Agropyron pectiniforme by Morphological Traits and RAPD Markers

Iranian Journal of Plant Physiology

دوره 11، شماره 3، تیر 2021، صفحه 3671-3682   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2021.682500

نویسندگان

Ali Ashraf Jafari^{* 1}؛ Soheila Afkar²؛ Meysam Faraji³

¹Agronomy and Breeding Dept. Rangelands Research Division Research Institute of Forests and Rangelands

²Plant Breeding Department, Agriculture Faculty, Payame Noor University, Tehran, Iran

³MS Graduated, Islamic Azad University, Borujerd Branch, Iran

چکیده

This study was conducted to analyze genetic diversity in 15 natural populations of Iranian Agropyron pectiniforme using morphological traits and RAPDs (Random Amplification of Polymorphic DNA) molecular markers. Five primers out of the 10 were highly polymorphic and produced 128 polymorphic bands ranging in length of 500 to 2200 bp. According to AMOVA (Analysis of Molecular Variance) results, there was higher genetic variation between populations (53%) than within (47%) them. Cluster analysis based on RAPD data categorized the populations into five clusters. PCoA (Principal Coordinates Analysis) results showed the first four coordinates accounted for 95% of the total variation. The scatter of genotypes based on the first two components was agreement with cluster analysis results. There was non-significant mantel correlation coefficient between molecular and geographical data indicating classification of A. pectiniforme based on RAPD marker was not in accord with geographical distribution. With regard to morphological traits, characters including plant height and the number of stems per plants are suitable parameters for selection of potential parents in the breeding programs. The non-significant mantel correlation coefficient between molecular and morphological marker was observed, then suggested that the two marker systems give different estimates of genetic relations among populations. Finally, our findings demonstrate the feasibility of the RAPD technique for quantifying genetic distances among A. pectiniforme populations. It was concluded that, there is sufficient genetic variation between Iranian populations of Agropyron pectiniforme which these genotypes can be useful for germplasm improvement and cultivar development.

کلیدواژه‌ها

Agropyron pectiniforme؛ AMOVA؛ Geographic data؛ Molecular marker؛ Morphological marker

مراجع

Amini, F., A.F., Mirlohi and M.M., Majidi. 2016. 'The possibility of use of AFLP molecular markers and phenotypic traits to increase forage yield in Tall Fescue (Festuca arundinacea) breeding'. J Agri Sci Tech, 18: 1419-1429.

Arghavani, A., A. Asghari, and M., Shokrpour Chamanabad. 2010. 'Genetic diversity in ecotypes of two Agropyron species using RAPD markers'. Res J Environ Sci, 4 (1): 50-56.

Babu, C., J., Sundaramoorthi, G., Vijayakumar and S., Ganesh Ram. 2009. 'Analysis of genetic diversity in Napier grass (Pennisetum purpureum Schum) as detected by RAPD and ISSR markers'. J Plant Biochem Biot, 18 (2): 181-187.

Behroozian, M., A., Jafari and M., Farsi. 2013. 'RAPD Analysis of Genetic Variation within and among natural populations of two species of Dianthus L. (Caryophyllaceae) in NE Iran'. Iran J Bot, 19 (2): 194-201.

Byars, S.G., Y. Parsons and A.A., Hoffmann. 2009. 'Effect of altitude on the genetic structure of an Alpine grass, Poa hiemata.' Ann Bot, 103: 885–899.

Che, Y.H. and L.H., Li. 2007. 'Genetic diversity of prolamines in Agropyron mongolicum Keng indigenous to northern China'. Genet Resour Crop Evol, 54: 1145-1151.

Cortese, L.M., J., Honig,C., Miller and S.A., Bonos. 2010. 'Genetic diversity of twelve swiss grass populations using molecular and morphological markers'. Bioenergy Res, 3: 262–271.

Dice, L.R. 1945. 'Measures of the amount of ecologic association between species'. Ecology, 26(3): 297-302.

Dorri, P., S. Khavari- Khorasani, M. Valizadeh and P. Taheri. 2014. 'The study of inheritance and gene effects on yield and agronomic traits of early generations of genetic maize Dehghan (KSC400)'. Plant Gen Res. 1. 2: 33-42. (In Persian)

Ebrahimian, M., M.M., Majidi and A.F., Mirlohi. 2012. 'Clonal evaluation and estimation of genetic similarity of tall fescue genotypes (Festuca arundinaceae)'. J Plant Prod, 19 (3): 91-108. (In Persian)

Falconer, D. S. 1989. 'Introduction to quantitative genetics'. Logman Scientific and technical logman house, Burnt Mill, Harlow, Essex, England.

Farshadfar, M. 2012. 'Genetic variability and Karyotype analysis of some Agropyron species'. Ann Biol Res, 3 (3):1515-1523.

Friebe, B., K.S., Gill, N.A., Tuleen and B.S., Gill. 1996.' Transfer of wheat streak mosaic virus resistance from Agropyron intermedium into wheat'. Crop Sci, 36:857-861.

Greene, S.L., M., Gritsenko and G.,Vandemark. 2004. 'Relating morphologic and RAPD marker variation to collection site environment in wild populations of red clover (Trifolium pratense L.).' Genet Resour Crop Evol, 51:643–653.

Grljusic, S., S., Bolaric, S., Popovic, T., Cupic, M., Tucak and V., Kozumplik. 2008. 'Comparison of morphological and RAPD markers in evaluation of red clover (Trifolium pretense L.) changes caused by natural selection'. Period Biol, 110 (3): 237-242.

Gustafson, D.J., D.J., Gibson and D.L., Nickrent. 1999. 'Random amplified polymorphic DNA variation among remnant big bluestem (Andropogon geradii Vitman) populations from Arkansas Grand Prairie'. Mol Ecol, 8: 1693-1701.

Jafari A. A., Elmi, A. and Bakhtiari, M. 2014.'Evaluation of yield and quality traits in 17 populations of tall wheatgrass (Agropyron elongatum) grown in rain fed area of Iran, under two cutting management'. Romanian Agricultural Research 31:49-58.

Johnson, H.W., H.F., Robinson and R.E., Comstock. 1955. 'Estimates of genetic and environmental variability in soybeans'. Agron J, 47: 314-318.

Kamm, U., P., Rotach, F., Gugerli, M., Siroky, P., Edwards and R., Holderegger. 2009.' Frequent longdistance gene flow in a rare temperate forest tree (Sorbus domestica) at the landscape scale'. Heredity, 103: 476–482.

Khan, S., K., Parveen and S., Goyal. 2011. 'Induced mutations in chickpea morphological mutants'. Front Agr China, 5: 35-39.

Kothera, L., C.M., Richards and S.E., Carney. 2007. 'Genetic diversity and structure in the rare Colorado endemic plant Physaria bellii Mulligan (Brassicaceae)'. Conserv Genet, 8: 1043–1050.

Landguth, E.L., B.C., Fedy, S.J., Oyler-Mccance, A.L., Garey, S.L., Emel, M., Mumma, H.H., Wagner, M.J., Fortin and S.A., Cushman. 2012. 'Effects of sample size, number of markers, and allelic richness on the detection of spatial genetic pattern'. Mol Ecol Resour, 12: 276-284.

Li, H., B., Jiang, J., Wang, Y., Lu, J., Zhang, C., Pan, X., Yang, X., Li, W., Liu and L., Li. 2017. 'Appling of novel powdery mildew resistance genes from Agropyron cristatum chromosome 2P'. Theor Appl Genet, 130 (1): 109-121.

Li, X., Y., Huang, J., Li and H., Corke. 2001. 'Characterization of genetic variation and relationships among Choix germplasm accessions using RAPD markers'. Genet Resour Crop Evol, 48: 189-194.

Majidi, M.M., A., Milohi and F., Amini. 2009. 'Genetic variation, heritability and correlations of agro-morphological in tall fescue (Festuca arundinacea).' Euphytica, 167: 323-331.

Mazid, M.S., M.Y., Rafii, M.M., Hanafi, H.A., Rahim and M.A., Latif. 2013. 'Genetic variation, heritability, divergence and biomass accumulation of rice genotypes resistant to bacterial blight revealed by quantitative traits and ISSR markers'. Physiol Plant, 149: 432-447.

Mohammadi, R., M.M., Khayyam-Nekouei, M.M., Majidi and A.F., Mirlohi. 2011. 'Estimation of yield potential and genetic variation of orchard grass genotypes (Dactylic glomerata)'. J Crop Prod, 3 (2): 139-158. (In Persian)

Mohsin, T., N., Khan and F.N., Naqvi. 2009. 'Heritability, phenotypic correlation and path coefficient studies for some agronomic characters in synthetic elite lines of wheat'. J Food Agric Environ, 7 (3&4): 278-282.

Nagl, N., K., Taski-Ajdukovic, A., Baburski, I., Seccareccia, D., Milic and S., Katic. 2011. 'Estimation of the genetic diversity in tetraploid alfalfa populations based on RAPD markers for breeding purposes'. Int J Mol Sci, 12: 5449-5460.

Nei, M. 1972. 'Genetic distance between populations'. Am Nat, 106: 283-292.

Padmaja, D., K., Radhika, S.L., Rao and V., Padma. 2008. 'Studies on variability, heritability and genetic advance for quantitative characters in rice (Oryza sativa L.)'. J Genet Res, 21(3):196-198.

Rahimi Analoujeh, N., A., Alizadeh and A., Namayande. 2014. 'RAPD molecular markers used to assess genetic and morphological diversity of some Satureja hortensis L.' masses. JAEBS, 4(1): 90-95.

Refoufi, A and M.A., Esnault. 2008. 'Population genetic diversity in the polyploidy complex of wheat grasses using isoenzyme and RAPD data'. Biol Plant, 52 (3): 543-547.

Rimoldi, F., P.S., Vidigal Filho, M.V., Kvitschal, M.C., Gonçalves-Vidigal, A.J., Prioli,S.M.A., Prioli and T.R., Costa. 2010. 'Genetic divergence in sweet cassava cultivars using morphological agronomic traits and RAPD molecular markers'. Braz Arch Biol Technol, 53(6): 1477-1486.

Saghai–Maroof, M.A., K.M., Soliman, R.A., Jorfenden and R., Allars. 1984. 'Ribosomal DNA spacer-Length polymorphism inbarley,Mendelian inheritance, chromosomal location, and population dynamics'. PNAS, 81: 8014-8018.

Salehi Shanjani, P., A.A., Jafari and R., Jahanbaz. 2015. 'Investigation of genetic variations among crested wheatgrass species base of agronomical traits and total leaf protein'. Jour. Range Sci, 5(3): 165-180.

Sharma, H.C., S.G., Aylward and B.S., Gill. 1987. 'Partial amphiploid from Triticum aestivum × Agropyron scirpeum cross'. Bot Gaz, 148 (2): 258-262.

Sharma, H.C. and P.S., Baezinger. 1986. 'Production, morphology and cytogenetic analysis of Elymus caninus (Agropyron caninum) × Triticum aestivum F1 hybrids and backcross-1 drrivatives'. Theor Appl Genet, 71: 750-756.

Sharma, H.C., B.S., Gill and J.K., Uyemoto. 1984. 'High levels of resistance in Agropyron species to Barley yellow dwarf and Wheat streak mosaic viruses'. Phytopath, 110: 143-147.

Shirvani, H., A.R., Etminan and H., Safari. 2014. 'Evaluation of variability and genetic parameters in morphological traits Agropyron trichophorum using multivariate statistical analysis'. Int J Biol Sci, 4 (2): 125-132.

Sihag, R., J.S., Hooda, R.D., Vashishtha and B.P.S., Malik. 2004. 'Genetic divergence in soybean (Glycine max L)'. Ann Biol, 20 (1): 17-21.

Singh, R.K., and Chaudhary, B.D. 1985. 'Biometrical methods in quantitative analysis'. Kalayani Publishers. New Delhi.

Szenejko, M. and M., Rogalski. 2015. 'Characterization of morphological triats and RAPD polymorphism in selected forms of Kentuchy bluegrass (Poa pratensis)'. Biodiver Res Conserv, 37: 1-10.

Taghizadeh, R., A.A., Jafari, A.A., Imani, A., Asghari and R., Choukan. 2011.' Investigation of genetic variability in Iranian populations of desert wheatgrass (Agropyron desertorum) based on morphological and RAPD markers'. IJRFPBGR, 19: 85–100. (In Persian).

Tucak, M., S., Popovic, S., Grljusic, S., Bolaric and V., Kozumplik. 2008. 'Genetic diversity of alfalfa (Medicago spp) estimated by molecular markers and morphological characters'. Period Biol, 110 (3): 243-249.

Tuhina-Khatun, M., M.M., Hanafi, M.R., Yusop, M.Y., Wong, F.M., Salleh and J., Ferdous. 2015. 'Genetic Variation, Heritability, and Diversity Analysis of Upland Rice (Oryza sativa L.) Genotypes Based on Quantitative Traits'. BioMed Res Int: 1-7

Tun,a M., D.K., Khadka, M., Shrestha, K., Arumugahathan and A., Golan-Goldhirsh. 2004. 'Characterization of natural orchardgrass (Dactulis glomerata L.) Population of the Thraace Region of Turkey based on ploidy and DNA'. Euphytica, 135: 39-46.

Varshney, R.K., A., Garner and M.E., Sorrells. 2005. 'Genomics-assisted breeding for crop improvement'. Trends Plant Sci, 10: 621-630.

Vieira, E.A., C.M., Castro, A.C., Oliveira, F.I.F., Carvakho, P.D., Zimmer and L.F., Martins. 2004. 'Genetic structure of annual ryegrass (Lolium multiflorum) populations estimated by RAPD'. Sci Agric, 61 (4): 407-413.

Wang, J.L.,Y.B., Gao, N.X., Zhao, A.Z., Ren, W.B., Ruah, L., Chen, J.L., Liu and C.L., Li.2006. 'Morphological and RAPD analysis of the dominant species StipakryloviiRoshev.' In inner Mongolia steppe. BotStud,47: 23-35.