Investigation Phenol, Flavonoids and Antioxidant Activity Content of Capparis spinosa in Three Natural Habitats of Sistan and Baluchestan Province, Iran

Journal of Rangeland Science

مقاله 8، دوره 12، شماره 2، تیر 2022، صفحه 191-204   

نوع مقاله: Research and Full Length Article

شناسه دیجیتال (DOI): 10.30495/rs.2022.682941

نویسندگان

Morteza Saberi^{* 1}؛ Nadia Kamali²؛ Farajollah Tarnian³؛ Ahmad Sadeghipour⁴

¹Range and Watershed Management Department, Water and Soil Collage, University of Zabol

²Assistant Professor, Rangeland Research Division, Research Institute of Forests and Rangelands, AREEO, Tehran, Iran

³Assistant Professor, Department of Range and Watershed Management, Faculty of Agriculture and Natural Resources, Lorestan University, Khorranabad, Iran

⁴Assistant Professor, Desert Studies Faculty, Semnan University

چکیده

Capparis spinosa L. is a shrub plant that in addition to its forage use, has protective importance to prevent soil erosion in desert areas and importance values in treating many diseases as well. The aim of this study was to investigate the amount of phenol, flavonoids and antioxidant activity in different organs of C. spinosa in Sistan, Iranshahr and Saravan counties, Iran. Morphological traits (number of fruits, wet weight of fruit, dry weight of fruit, fruit diameter, number and length of branches, plant height, leaf length, leaf width and root depth) in each habitat were measured from four individuals of C. spinosa randomly. In order to perform phytochemical tests, different parts of the plant (stem, leaves, flowers, fruits, and roots) were randomly collected from the habitats in the post-flowering stage in June 2019. The total phenol and flavonoid content of all methanolic extracts were measured using the spectrophotometric method and antioxidant activity was determined using the free radical trap method. Data analysis was performed as factorial experiment based on completely randomized design in four replications. Rresults significant differences between different plant organs (P<0.01) in aspect of the antioxidant activity, the amount of total phenol and flavonoids. Also, there was a significant plant organs by habitats interaction (P<0.01). The results of the mean comparison showed the highest total phenol and total flavonoids were obtained from the methanolic extract of the flower 82.8 mg of quercetin equivalent per gram dry weight and 64.3 mg of gallic acid/g of dry weight in Sistan region, respectively, and the highest antioxidant activity was 15.7% in the fruit in Iranshahr region. According to the results, the obtained methanolic extract of C. spinosa flower and fruit in Sistan natural habitats is recommended to the treatment of diseases as a potential source of natural antioxidants.

کلیدواژه‌ها

Medicinal Plant؛ Methanolic extracts؛ Morphological traits؛ Treatment

مراجع

Abderrahmane, S., Daoud, H. and Hani, B., 2011. Radical, metal-chelating and antibacterial activities of methonolic extract of Capparis spinosa buds. Advances in Environmental Biology, 5(2): 287-1.

Baghiani, A., Ameni, D., Boumerfeg, S., Adjadj, M., Djarmouni, M., Charef, N., Khennouf, S. and Arrar, L., 2012. Studies of antioxidants and xanthine oxidase inhibitory potentials of root and aerial parts of medicinal plant Capparis spinosa L. American Journal of Medicine and Medical Sciences, 2(1): 25-32

Bajalan, I., 2016. Investigation of Environmental Factors Regarding the Essential Oil and Antibacterial Activity of Lavandin (Case Study: Jowkar, Khoramabad, Alashtar and Aligoodarz from Iran). Rangeland Science Journal, 6(4): 344-354.

Beketov, M. A. and Liess, M., 2005. Acute contamination with esfenvalerate and food limitation: chronic effects on the mayfly, Cloeon dipterum. Environmental Toxicology and Chemistry, 24: 1281- 1286.

Burtis, M. and Bucar, F., 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14: 323-328.

Calis, I., Kuruzum, A. and Ruedi, P. D., 1999. 1H- Indole-3-acetonitrile glycosides from Capparis spinosa fruits. Phytochemistry, 50: 1205–1208.

Carbone, K., Giannini, B., Picchi, V., Lo Scalzo, R. and Cecchini, F., 2011. Phenolic composition and free radical scavenging activity of different apple varieties in relation to the cultivar, tissue type and storage. Food Chemistry, 127: 493– 500.

Chang, C., Yang, M., Wen, H. and Chern, J., 2002. Estimation of total flavonoid content in Propolis by two complementary calorimetric methods. Journal of Food and Drug Analysis, 10: 178-18.

Chedraoui, S., Abi-Rizk, A., El-Beyrouthy, M., Chalak, L., Ouaini, N. and Rajjou, L., 2017. Capparis spinose L. in A Systematic Review: A Xerophilous Species of Multi Values and Promising Potentialities for Agrosystems under the Threat of Global Warming. Frontier in Plant Science, 8: 1845.

Davari, A., Solouki, M. and Fazelinasab, B. 2018. Effects of jasmonic acid and titanium dioxide nanoparticles on process of changes of phytochemical and antioxidant in genotypes of Satureja hortensis L. Eco-Phytochemical Journal of Medical Plants, 5: 1-20. (In Persian).

El Amri, N., Errachidi, F., Bour, A., Bouhaddaoui, S. and Chabir, R., 2019. Morphological and Nutritional Properties of Moroccan Capparis spinosa Seeds. Hindawi, the Scientific World Journal, Article ID 8594820: 1-8.

Fakhri, M., Bakhshi Khaniki, Gh. and Sadeghi, M., 2008. A Survey on ecological character of Capparis spinosa L. Bushehr Province. Pajouhesh & sazandegi, 80: 169-175. (In Persian).

Fazelinasab, B., Moshtaghi, A. and Forouzandeh, M., 2019. Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs. Scientific Journal of Ilam University of Medical Sciences, 27(3): 14-26. (In Persian).

Fazelinasab, B., Rahnama, M. and Mazarei, A., 2017. Correlation between Antioxidant Activity and Antibacterial Activity of Nine Medicinal Plant Extracts. Journal of Mazandaran University of Medical Sciences, 27: 63-78. (In Persian).

Gairola, S., Shariff, N. M., Bhatt, A. and Kala, C. P., 2010. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. Journal of Medicinal Plants Research, 4(18): 1825 - 9.

Güleryüz, M., Özkan, G. and Ercisli, S., 2009. Caper (Capparis spp.) growing techniques and economical importance, Ataturk University Agricultural Faculty Department of Horticulture Erzurum-Turkey, International Symposium on Sustainable Development, Available at: http:// eprints.ibu.edu.ba/484/.

Hamed, A. R., Abdel-Shafeek, K. A., Abdel-Azim, N. S., Ismail, S. I. and Hammouda, F. M., 2007. Chemical investigation of some capparis species growing in Egypt and their antioxidant activity. Evidence-Based Complementary and Alternative Medicine, 4: 25–28.

Iranmanesh, M., Najafi, Sh. and Yousefi, M., 2010. Ethnobotany study of medicinal plants in Sistan region. Journal of Herbal Drugs, 2: 61-68.

Jovancevic, M., Balijagic, J., Menkovic, N., Savikin, K., Zdunic, G., Jankovic, T. and Dekic-Ivankovic, M., 2011. Analysis of Phenolic compounds in wild populations of bilberry (Vaccinium myrtillus) from Montenegro. Journal of Medicinal Plants Research, 5(6): 910 -4.

Karimian, V., Vahabi, M. R., Roustakhiz, J. and Nodehi, N., 2017. Identification of some ecological factors affecting essential oil of Verbascum songaricum schrenk shoots (Case Study: Rangelands of Isfahan and Kohgiluyeh and Buyerahmad Provinces, Iran). Rangeland Science Journal, 7(2): 183-194.

Khalasi Ahwazi, L., Heshmati, G. H., Zofan, P., Akbarlou, M., 2016. Total phenol, flavonoid contents and antioxidant activity of Gundelia tournefortii L. in different phenological stages and habitats of north east of Khuzestan province. Eco-phytochemical Journal of Medicinal Plants, 4 (1): 33-46. (In Persian).

Khazaee, F. A., Etebarian, H. R., Roustaee, A. and Alizadeh, A., 2011. Study of changes in peroxidase enzyme and total phenol in golden delicious apple fruits inoculated with an antagonistic isolate of Pseudomonas fluorescens and Penicillium expansum the causal agent of apple blue mould. Seed and Plant Production Journal, 26: 419-433. (In Persian).

Ksouri, R., Falleh, H., Megdiche, W., Trabelsi, N., Hamdi, B., Chaieb, K., Bakhrouf, A., Magné, C. and Abdelly, C., 2009. Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical Toxicology, 47: 2083–2091.

Kulisic-Bilusic, T., Schmoller, I., Siracusa, L. and Ruberto, G., 2012. The anticarcinogenic potential of essential oil and aqueous infusion from caper (Capparis spinosa L.). Food Chemistry, 132(1): 261–267.

Laurel, F. R., Servio, R. P., Valerie, B. K., 1999. Direct and indirect effects of climate change on St. John's wort, Hypericum perforatum L. (Hypericaceae). Oecologia, 120: 113-122.

Lisiewska, Z., Kmiecik, W. and Korus, A., 2006. Content of vitamin C, carotenoids, chlorophylls and polyphenols in green parts of dill (Anethum graveolens L.) depending on plant height. Journal of Food Composition and Analysis, 19: 134–140.

Matkowski, A., Zielińska, S., Oszmiański, J. and Lamer-Zarawska, E., 2008. Antioxidant activity of extracts from leaves and roots of Salvia miltiorrhiza Bunge, S. przewalskii Maxim., and S. verticillata L. Bioresouce Technology, 99: 7892–7896

Mishra, S. N., Toma, P. C. and Lakra, N., 2007. Medicinal and food value of Capparis. Indian Journal of Traditional Knowledge, 6(1): 230-238.

Moghadamnia, Y., Mousavi Kani, S. N., Ghasemi-Kasman, M., Kazemi Kani, M. T. and Kazemi, S., 2019. The Anticancer Effects of Capparis spinosa Hydroalcoholic Extract. Avicenna Journal of Medical Biotechnology, 11(1): 43-47.

Mollica, A., Stefanucci, A., Macedonio, G., Locatelli, M., Luisi, G., Novellino and E., Zengin, G., 2019. Chemical composition and biological activity of Capparis spinosa L. from Lipari Island. South African Journal of Botany, 120: 135–140. https://doi.org/10.1016/j.sajb.2018.02.397

Mollica, A., Zengin, G., Locatelli, M., Stefanucci, A., Mocan, A., Macedonio, G., Carradori, S., Onaolapo, O., Onaolapo, A. and Adegoke, J., 2017. Anti-diabetic and anti-hyperlipidemic properties of Capparis spinosa L.: in vivo and in vitro evaluation of its nutraceutical potential. Journal of Functional Foods, 35: 32–42.

Moufid, A., Farid, O. and Eddouks, M., 2015. Pharmacological Properties of Capparis Spinosa Linn. International Journal of Diabetology & Vascular Disease Research, 3(5): 99-104. doi: http://dx.doi.org/10.19070/2328-353X-1500020.

Mousavi, S. H., Hosseini, A., Bakhtiari, E., Rakhshandeh, H., 2016. Capparis spinosa reduces Doxorubicin-induced cardio-toxicity in Cardiomyoblast cells. Avicenna Journal of Phytomedicine, 6(5): 489-494.

Nabavi, S. F., Maggi, F., Daglia, M., Habtemariam, S., Rastrelli, L. and Nabavi, S. M., 2016. Pharmacological Effects of Capparis spinosa L. Phytotherapy Research, 30(11):1733-1744. doi: 10.1002/ptr.5684.

Najafi, Sh. and Esmail Zadeh Bahabadi, S., 2016. Essential oil composition and antioxidant optimization of Capparis spinosa L. fruit in Sistan region. Eco-Phytochemical Journal of Medicinal Plant. 4(15): 35-45. (In Persian).

Panico, A. M., Cardile, V., Garufi, F., Puglia, C., Bonina, F. and Ronsisvalle, G., 2005. Protective effect of Capparis spinosa on chondrocytes. Life Science, 77: 2479–2488.

Prior, R. L. and Cao, G., 2000. Antioxidant phytochemicals in fruits and vegetable diet and health implications. Horticultural Science, 35: 582-88.

Rahnavard, R. and Razavi, N., 2017. A review on the medical effects of Capparis spinosa L. Advanced Herbal Medicine, 2(1): 44-53.

Rashedi, H., Amiri, H. and Gharezi, A., 2015. Assessment of phytochemical and antioxidant properties of the Capparis spinosa L. in Khuzestan province. Journal of Inflammatory Diseases, 18(6): 11-1. (In Persian).

Rehman, S. U., Choe, K. and Yoo, H. H. 2016. Review on a traditional herbal medicine, Eurycoma longifolia Jack (Tongkat Ali): Its Traditional uses chemistry, evidence - based pharmacology and toxicology. Molecules, 21(331): 1-31.

Rezzan, A., Ozan, E. and Huseyin, S., 2013. Phenolic components, antioxidant activity, and mineral analysis of Capparis spinosa L. African Journal of Biotechnology, 12(47): 6643-9

Saberi, M., Niknahad-Gharmakher, H., Heshmati, H., Barani, B. and Shahriari, A., 2018. Evaluation of the Content Changes of Some Active Ingredients Extracts of Citrullus colocynthis Organs from Two Habitats of Sistan and Baluchestan Province. Journal of Plant Ecosystem Conservation, 5(10): 49-64. (In Persian).

Shan, B., Cai, Y Z., Sun, M. and Corke, H., 2005. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. Journal of Agricultural and Food Chemistry, 53: 7749-7759.

Sharrif moghaddasi, M., Haddad Kashani, H. and Azarbad, Z., 2012. Capparis spinosa L. Propagation and Medicinal uses. Life Science Journal, 9(4): 684-686.

Singleton, V. L., Orthofer, R. and Lamuela- Raventos, R. M., 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178.

Sparks, D. L., Page, A. L., Helmke, P. A., Loeppert, R. H., Soltanpour, P. N., Tabatabai, M. A., Johnston, C. T., Sumner, M. E., 2001. Methods of Soil Analysis: Part 3 Chemical Methods, 5.3. The Soil Science Society of America, Inc., American Society of Agronomy, 677 South Segoe Road, Madison, Wisconsin 53711 USA. 1309p.

Sukhdev, S., Handa, S., Singh, P., Gennaro, Kh., Dev, L. and Dutt, R., 2008. Extraction technologies for medicinal and aromatic plants, International center for science and high technology, 260p.

Tlili, N., Elfalleh, W., Saadaoui, E., Khaldi, A., Trike, S. and Nasri, N., 2011. The Caper (Capparis L.): Ethnopharmacology, phytochemical and pharmacological properties. Fitoterapia, 82(2): 93-101.

Trabelsi, N., Falleh H., Jallali I., Daly A., Hajlaoui H., Smaoui A, Abdelly C., Ksouri R., 2012. Variation of phenolic composition and biological activities in Limoniastrum monopetalum L organs. Acta Physiologiae Plantarum, 34: 87-96.

Vahid, H., Rakhshandeh, H. and Ghorbani, A., 2017. Antidiabetic properties of Capparis spinosa L. and its components. Biomedicine & Pharmacotherapy, 92: 293–302.

Zhang, H. and Zheng, F. M., 2018. Phytochemical and Pharmacological Properties of Capparis spinosa as a Medicinal Plant. Nutrients, 10(116): 1-14. Doi: 10.3390/nu10020116.

Zia-Ul-Haq, M., Cavar, S. and Qayum, M., 2011. Compositional studies: antioxidant and antidiabetic activities of Capparis decidua (Forsk.) Edgew. International Journal of Molecular Science, 12(12): 8846-61.