Quality assessment and molecular identification of bacteria across the black tiger shrimp (Penaeus monodon) supply chains in Southern Bangladesh

International Aquatic Research

مقاله 2، دوره 13، شماره 3، آذر 2021، صفحه 173-184   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2021.1924974.1142

نویسندگان

Md. Sazedul Hoque^{* 1}؛ Md. Abu Sayed Ripon²؛ Md. Belal Hossain³؛ Md. Arifur Rahman⁴؛ Md. Mahmudul Hasan²؛ Biplob Dey Mithun²؛ Avirup Saha⁵؛ Suprakash Chakma²؛ Mst. Niloy Jaman²؛ Akhinur Rahman⁶؛ Muhammad Manjurul Karim⁶

¹Department of Fisheries Technology, Faculty of Fisheries, Patuakhali Science and Technology University, Bangladesh

²Department of Fisheries Technology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

³Department of Food Microbiology, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

⁴Department of Fisheries Biology and Genetics, Patuakhali Science and Technology University, Dumki, Patuakhali-8602, Bangladesh

⁵Bangladesh Food Safety Authority, Dhaka, Bangladesh

⁶Department of Microbiology, University of Dhaka, Dhaka, Bangladesh

چکیده

Black Tiger Shrimp (Penaeus monodon) is one of the leading export commodities in Bangladesh. The industry, however, is frequently beset with quality deterioration and microbial diseases owing to various reasons. Here, we analyzed the sensory and nutritional qualities of shrimp, the total bacterial count, and molecular identification of pathogenic bacteria in samples collected from different supply chain actors (SCA), including the gher (farms), depots, wholesalers, retailers, and the processing industry in Khulna, Bangladesh. While the Sensory Quality Indices (SQI) of shrimps collected from farms, depots, and processing plants were found ‘excellent and highly acceptable’, the rating for wholesale and retail markets were ‘good and acceptable’. Nutritionally, higher protein (21.59%), lipid (1.47%), and ash (1.97%) contents were found in shrimps collected from farms than that of other SCAs. Heterotrophic bacterial count (cfu/g) differed in various SCAs: farms 3.75±0.31 (×10⁶), depots 3.95±0.92 (×10⁶), wholesale markets 5.03±0.35 (×10⁶), retail markets 4.82±0.40 (×10⁶), and the processing industry 3.78±0.25 (×10⁶). The presence of Ralstonia spp and Pseudomonas spp, confirmed by 16S rDNA sequencing were found in different SCAs. Overall, the quality of shrimp appeared better in farms than that of other SCAs, indicating that a lack of proper hygiene, sanitation, and post-harvest handling practices could be the likely impediments for quality deterioration.  

کلیدواژه‌ها

Shrimp؛ Supply chain؛ Sensory quality؛ 16S rDNA sequencing, Microbial quality؛ Nutritional quality