بررسی تنوع ژنتیکی لاکتوباسیلوس‌های جدا شده از نمونه‌های شیر گاو به روش RAPD-PCR

فصلنامه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی

دوره 1، شماره 1، شهریور 1400، صفحه 1-16   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/qafj.2021.685558

نویسندگان

حکیمه قلعه خانی¹؛ معصومه حاجی رضایی^{* 2}؛ آرتادخت توکلی²

¹کارشناسی ارشد، گروه میکروبیولوژی، واحد کرمان، دانشگاه آزاد اسلامی، کرمان، ایران

²استادیار، گروه میکروبیولوژی، واحد کرمان، دانشگاه آزاد اسلامی، کرمان، ایران

چکیده

محصولات لبنی مناطق مختلف می­توانند منبعی برای سویه­های جدید لاکتوباسیلوس باشند، بنابراین شناسایی مولکولی و بررسی تنوع ژنتیکی آنها می­تواند گام موثری در شناسایی ذخیره لاکتوباسیلوس­های بومی با خصوصیات عملکردی ویژه و به کارگیری آنها در محصولات لبنی صنعتی شود. هدف از این تحقیق جداسازی لاکتوباسیلوس­ها از فلور موجود در شیر گاو شهرستان نرماشیر و بررسی تنوع ژنتیکی آنها می­باشد. بدین­منظور تعداد 21 نمونه شیر از مناطق مختلف این شهرستان جمع­آوری و با روش­های مرسوم فنوتیپی و بیوشیمیایی لاکتوباسیلوس­ها جداسازی شدند. به منظور غربالگری و بررسی تنوع ژنتیکی لاکتوباسیلوس­های جدا شده، پنج پرایمر RAPD به طور تصادفی انتخاب گردید و پس از PCR، تجزیه و تحلیل تنوع ژنتیکی و فیلوژنتیکی با استفاده از نرم­افزار Pyelph انجام و درخت فیلوژنی رسم شد. تجزیه خوشه­ای داده­های مولکولی توانست جدایه­ها را در فاصله ژنتیکی 10 به هفت گروه مجزا تقسیم­بندی کند. جدایه­های K3 و K4 در گروه یک و جدایه­های K21 و K13 و K11 در گروه چهار قرار گرفتند. بقیه جدایه­ها در گروه­های مجزا قرار گرفتند. از آنجائیکه جدایه­های K3 و K4 از یک ناحیه و جدایه­های K21 و K13 نیز از یک ناحیه جمع­آوری شده­اند، قرار گرفتن آنها در یک گروه منطقی به نظر می­رسد. نتایج بدست آمده از این تحقیق بیانگر تنوع ژنتیکی بالای جدایه­های بومی شهرستان نرماشیر و مناسب بودن تکنیک RAPD-PCR برای مطالعات مشابه می­باشد.

کلیدواژه‌ها

لاکتوباسیلوس؛ تنوع ژنتیکی؛ RAPD-PCR؛ درخت‎ فیلوژنی

مراجع

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