بررسی فیتوشیمیایی، آنتی‌اکسیدانی و مهارکنندگی آنزیمی عصاره‌های مختلف گیاه دارویی .Salvia hypoleuca Benth

اکوفیتوشیمی گیاهان دارویی

مقاله 7، دوره 9، شماره 4 - شماره پیاپی 36، اسفند 1400، صفحه 93-109   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/ejmp.2021.1940036.1650

نویسندگان

بهور اصغری^{* 1}؛ سودابه مفاخری²؛ مجید قربانی نهوجی³

¹دانشیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران.

²استادیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران

³استادیار، مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران

چکیده

گونه ­های مختلف جنس مریم گلی (Salvia)، گیاهانی هستند که از اسانس­ها و عصاره ­های آنها در صنایع مختلف دارویی، غذایی و آرایشی به شکل گسترده­ای استفاده می­شود. Salvia hypoleuca Benth. یک گونه بومی این جنس در ایران است که اندام هوایی آن در مرحله گلدهی در تابستان 1399 از اطراف شهرستان الموت استان قزوین جمع­آوری شد. در مطالعه حاضر محتوای فنل کل، فلاونوئید، ساپونین و تانن، چهار عصاره هگزانی، اتیل­استاتی، متانولی و آبی که با استفاده از روش ماسراسیون تهیه شده بودند، مورد بررسی قرار گرفته است. خواص آنتی­اکسیدانتی این عصاره ­ها نیز با استفاده از روش ­های DPPH، ABTS، فسفومولیبدنیوم و کیلیت­کنندگی آهن و قدرت مهارکنندگی آنزیم ­های آلفا-آمیلاز، آلفا-گلوکوزیداز و تیروزیناز با استفاده از روش اسپکتروفتومتری اندازه­گیری شد. بر اساس نتایج به دست آمده عصاره اتیل­استاتی به ترتیب با مقادیر 07/87 و 84/163 میلی­گرم معادل گالیک اسید و ساپونین کوئیلاجا بر گرم عصاره دارای بالاترین مقدار محتوای فنلی و ساپونینی بود. عصاره آبی این گیاه نیز با دارا بودن به ترتیب 97/33 و 34/5 میلی­گرم معادل کوئرستین و کتچین برگرم عصاره دارای بالاترین محتوای فلاونوئیدی و تاننی متراکم بود. عصاره اتیل­استاتی بالاترین قدرت به دام­اندازی رادیکال­های آزاد ABTS، فعالیت آنتی­اکسیدانتی تام، قدرت کلیت کنندگی یون فروس و قدرت بازدارندگی فعالیت آنزیم­های آلفا-آمیلاز، آلفا-گلوکوزیداز و تیروزیناز را از خود نشان داد. پتانسیل بالای عصاره اتیل­استاتی در خواص بیولوژیکی نظیر آنتی­اکسیدانتی و ضدرادیکالی، می­تواند به محتوای بالای متابولیتی آن به ویژه، ترکیبات فنولی و ساپونینی مربوط باشد. به این ترتیب می­توان نتیجه گرفت که این گیاه منبعی غنی از متابولیت­ های ثانویه فعال است که بهترین حلال استخراجی برای این ترکیبات اتیل­استات می­باشد. نتایج این تحقیق پتانسیل احتمالی گیاه S. hypoleuca را برای استفاده ­های دارویی، به ویژه برای مقابله با دیابت و بیماری­های پوستی تأیید کرد.
 

کلیدواژه‌ها

مریم گلی؛ عصاره؛ محتوای متابولیتی؛ مهار آنزیمی

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