Phytochemical composition, antioxidant, and antibacterial activity of the Philippine marine green alga (Ulva pertusa)

International Aquatic Research

دوره 14، شماره 1، خرداد 2022، صفحه 51-62   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2022.1946410.1217

نویسندگان

Rolando Pakingking Jr.^{* 1}؛ Roselyn Usero²؛ Evelyn Grace de Jesus-Ayson³؛ Dan Joseph Logronio⁴؛ Christopher Marlowe Caipang⁵

¹Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/ AQD), Philippines

²Negros Prawn Producers Marketing Cooperative, Inc., Bacolod City 6100, Negros Occidental, Philippines

³Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/AQD), Tigbauan 5021, Iloilo, Philippines and College of Arts and Sciences, Central Philippine University, Jaro, Iloilo City 5000, Iloilo, Philippines

⁴Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC/AQD), Tigbauan 5021, Iloilo, Philippines

⁵College of Liberal Arts, Sciences, and Education and the Center for Chemical Biology and Biotechnology, University of San Agustin, General Luna St., Iloilo City, 5000, Philippines

چکیده

The phytochemical constituents, total phenolic and flavonoid contents, antioxidant and antimicrobial activities of the ethanolic extract of the marine green alga Ulva pertusa collected from floating fish net cages off the coast of Guimaras island, Philippines, were investigated. Qualitative phytochemical analysis revealed the presence of bioactive compounds including alkaloids, flavonoids, phenols, saponins, tannins, and terpenoids. Quantitatively, total phenolic and flavonoid contents of the extract were 20.54±2.08 mg gallic acid equivalent (GAE) g-1 and 539.07±6.36 mg rutin equivalent (RE) g-1, respectively. The antioxidant activity of the extract using 1,1-diphenyl-1-picrylhydrazyl (DPPH) assay exhibited a concentration-dependent radical scavenging capacity. Additionally, the ethanolic extract inhibited the growth of Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922, Streptococcus agalactiae, Aeromonas hydrophila, A. sobria, and Vibrio harveyi in a concentration-dependent manner with strong inhibitory activity at 100 mg mL-1 concentration. Taken together, U. pertusa contains bioactive compounds that possess potent antibacterial activity and remarkable antioxidant capacities. These substances are promising candidates that may be utilized in the synthesis of novel drugs.

کلیدواژه‌ها

Ulva pertusa؛ Antimicrobial activity؛ Antioxidant؛ Streptococcus agalactiae؛ Aeromonas species