اثر آستاگزانتین بر آسیب القاء‌شده توسط کلرید کادمیوم در بیضهء موش‌صحرایی نر بالغ نژاد ویستار

آسیب‌شناسی درمانگاهی دامپزشکی

دوره 16، 2 (62) تابستان، شهریور 1401، صفحه 127-140   

نوع مقاله: مقاله علمی پژوهشی

شناسه دیجیتال (DOI): 10.30495/jvcp.2022.1942072.1330

نویسندگان

مهوش حافظ‌امینی¹؛ اکرم عیدی²؛ سید پژمان مرتضوی^{* 3}؛ فتاح ستوده نژاد نعمت الهی⁴

¹دانش‌آموخته دکتری تخصصی فیزیولوژی، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

²استاد گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

³دانشیار گروه پاتولوژی، دانشکده دامپزشکی، واحدعلوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

⁴استادیار گروه زیست شناسی، واحدعلوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

نقش حفاظتی آستاگزانتین بر سلامتی انسان، از جمله پیشگیری از بیماری‌های قلبی- عروقی و جلوگیری از روند پیرشدن سلول‌ها، اشاره به خاصیت آنتی‌اکسیدانی بسیار قوی این ماده دارد. کادمیوم یکی از آلاینده‌های موجود در اکوسیستم­ها است. این فلز سنگین به عنوان یک توکسین بر سیستم تولیدمثل اثر گذاشته و سبب تخریب روند اسپرماتوژنز در بافت بیضه می­شود. هدف مطالعه حاضر، بررسی تأثیر آستاگزانتین بر آسیب بیضه­ای القاءشده توسط کلرید کادمیوم در موش صحرایی بود. بدین منظور، تعداد ۴۵ سر موش صحرایی نر بالغ نژاد ویستار به­صورت تصادفی به ۹ گروه تقسیم شدند. گروه کنترل سالم هیچ­گونه تیماری دریافت نکرد. گروه شاهد، سرم فیزیولوژی، گروه کنترل نابارور، کلرید کادمیوم (به میزان 1 میلی­گرم بر کیلوگرم وزن) به صورت تزریق درون صفاقی، 3 گروه‌ آستاگزانتین را با دوزهای 5، ۱۰ و ۲۰ میلی­گرم بر کیلوگرم وزن و 3 گروه‌ هم همزمان کلرید‌ کادمیوم و آستاگزانتین را با دوزهای 5، ۱۰ و ۲۰ میلی­گرم بر کیلوگرم به صورت گاواژ دریافت کردند. از هموژنات بافت بیضه جهت سنجش پارامترهای استرس ‌اکسیداتیو، به­عنوان نمونه‌های آزمون استفاده شد. پارامترهای مورد نظر به روش فتومتریک با دستگاه الایزا‌ ریدر و میکروپلیت ریدر بررسی شد. آستاگزانتین با مقادیر 10 و 20 میلی‌گرم بر کیلوگرم وزن، موجب افزایش آنزیم­های آنتی­اکسیدانی سوپراکسید دیسموتاز، گلوتاتیون‌ پراکسیداز و کاتالاز و همچنین کاهش میزان مالون‌دی‌آلدئید شد (001/0˂p). بنابراین، آستاگزانتین احتمالاً به عنوان یک آنتی­اکسیدان در محافظت از بیضه موش صحرایی در برابر استرس اکسیداتیو ناشی از کلرید کادمیوم عمل می­کند.

کلیدواژه‌ها

آستاگزانتین؛ کلرید کادمیوم؛ آسیب؛ بیضه؛ موش صحرایی

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