Dose-Dependent Impacts of Nano-Sized Ceria (CeO2) on Seed Germination, Early Growth and Physiological Parameters of Marigold Seedling

Journal of Ornamental Plants

مقاله 2، دوره 12، شماره 2، شهریور 2022، صفحه 101-114   

نوع مقاله: Research Paper

نویسندگان

Sedighe Jahani¹؛ Sara Saadatmand¹؛ Malihe Jahani^{* 1}؛ Homa Mahmoodzadeh²؛ Ramazan Ali Khavari-Nejad¹

¹Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

چکیده

Marigold is widely used as an ornamental-medicinal plant. Interaction between nanoparticles (NPs) and biological systems is one of the most promising areas of research in modern nanoscience and technology. Researchers have reported the uptake of cerium oxide or ceria (CeO₂) NPs by plants. The aim of this investigate was to determine the impacts of nanoceria on seed germination, growth and biochemical characteristics of 9-day-old seedling of marigold (Calendula officinalis L.). Seeds were germinated in Petri dishes containing eight various dosages of nanoceria (0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, and 3.2 g/L). After 9 days, early growth and biochemical parameters were measured. Results showed that seed germination, fresh and dry weights of seedling, and lengths of radicle, plumule, and seedling stimulated at 0.05 and/or 0.1 g/L of nanoceria but retarded at higher dosages (after 0.2 or 0.4 g/L) of NPs. The contents of H₂O₂, malondialdehyde (MDA) and lipoxygenase (LOX) activity incremented after 0.2 g/L of nanoceria. The activities of antioxidant enzymes and protein content were incremented at higher dosages of nanoceria. Also, the activity of phenylalanine ammonialyase (PAL), phenol content, and antioxidant capacity stimulated at 0.8 to 3.2 g/L of nanoceria. The proline content improved at 0.2-3.2 g/L of nanoceria. Altogether, the results confirmed the inducive oxidative stress of nanoceria that was accompanied by plant defense system include antioxidant enzymes, phenolic compounds and compatible osmolytes such as proline. These results showed that nanoceria at the low dosages (0.05 and/or 0.1 g/L) caused a positive induction on marigold germination and seedling growth but by increasing in its dosage (more than 0.2 g/L), the results was reversed and showed toxicity that forced the plant to activate its defense systems.

کلیدواژه‌ها

Calendula officinalis L؛ Germination percentage and rate؛ Nanotoxicity؛ Plant defense system؛ Radicle and plumule length

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