تاثیر ورزش هوازی و مصرف هم‌زمان داروی سربرولایزین (Cerebrolysin) بر حافظهء فضائی موش‌های صحرایی نر مدل پارکینسون

آسیب‌شناسی درمانگاهی دامپزشکی

دوره 16، 1 (61) بهار، خرداد 1401، صفحه 87-102   

نوع مقاله: مقاله علمی پژوهشی

شناسه دیجیتال (DOI): 10.30495/jvcp.2022.1954843.1363

نویسندگان

سید زانیار اطهری¹؛ میرعلیرضا نورآذر^{* 2}؛ داریوش مهاجری³

¹دانشجوی دکترای تخصصی فیزیولوژی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.

²استادیار فیزیولوژی، دانشکده دامپزشکی، واحد علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

³استاد پاتولوژی، دانشکده دامپزشکی، واحد علوم پزشکی تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

چکیده

اختلال در حافظه، یکی از عوارض بیماری پارکینسون است. سربرولایزین دارای اثرات نوروتروفیک و تحریک رشد نورونی می‌باشد. ورزش نیز باعث افزایش فاکتور­های رشد مغز و بهبود حافظه فضائی می‌شود. هدف از انجام مطالعه حاضر، ارزیابی تاثیر ورزش هوازی و مصرف هم‌زمان داروی سربرولایزین بر حافظه فضائی موش‌های صحرائی نر مدل پارکینسون بود. بدین منظور 36 سر موش صحرائی نر نژاد ویستار با وزن 20±250 به‌طور تصادفی به 6 گروه مساوی شامل شم جراحی، پارکینسونی (تزریق 6-هیدروکسی دوپامین، تک‌دوز یک­طرفه داخل جسم سیاه مغز به­میزان µg/ml 5/12)، کنترل مثبت (لوودوپا، به­میزان mg/kg 12خوراکی، به­مدت 21 روز)، تیمار سربرولایزین (به­میزان mg/kg 538 تزریق داخل صفاقی، به­مدت 21 روز)، ورزش تردمیل (روزانه به مدت 30 دقیقه، 60 درصد حداکثر اکسیژن مصرفی، به­مدت 21 روز) و تیمار سربرولایزین (به­میزان mg/kg 538 تزریق داخل صفاقی، به­مدت 21 روز) توام با ورزش (روزانه به مدت 30 دقیقه، 60 درصد حداکثر اکسیژن مصرفی، به­مدت 21 روز) تقسیم شدند. در پایان، آزمایش حافظه‌ فضائی با ماز آبی موریس در گروه‌های مورد مطالعه انجام شده و پس از آسان‌کشی، از بافت مغز موش­ها جهت مطالعه آسیب­شناختی ناحیه هیپوکامپ نمونه­برداری شد. داده‌ها توسط آزمون­های آماری تحلیل واریانس یک­طرفه (ANOVA) و توکی (Tukey) در سطح 05/0­p<­­، توسط نرم‌افزار گراف‌پد (Graphpad) تحلیل گردید. القاء پارکینسون باعث کاهش شاخص‌های حافضه فضائی و سربرولایزین باعث افزایش فاکتورهای حافظه فضائی ­گردید (05/0p<). همچنین ورزش هوازی پارامترهای مربوط به حافضه فضائی را همانند سربرولایزین بهبود بخشید (05/0p<). ورزش هوازی توأم با سربرولایزین هم بهترین تاثیر را در بهبود شاخص‌های حافظه فضائی داشت. نتایج آسیب­شناسی نیز همسو با نتایج حافظه فضائی بود. به­نظر می­رسد که ورزش هوازی و داروی سربرولایزین به‌طور همزمان، منجر به بهبود حافظه^­ء فضائی و تغییرات آسیب­شناختی هیپوکامپ در موش­های صحرائی مدل پارکینسون می­شود.

کلیدواژه‌ها

پارکینسون؛ ورزش هوازی؛ سربرولایزین؛ موش‌صحرایی؛ حافظه‌ء فضائی

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