Evolution of Biological Properties of Bioactive Diopside and Wollastonite for Bone Tissue Engineering

Journal of Advanced Materials and Processing

دوره 10، شماره 1 - شماره پیاپی 37، فروردین 2022، صفحه 39-56   

نوع مقاله: Research Paper

نویسندگان

Ruhollah Zamani Foroushani¹؛ Ebrahim karamian^{* 1}؛ Mohammad Rafienia²

¹Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran

²Biomaterial, Nanotechnology and Tissue engineering Department of Advanced Medical Technology, Isfahan University of Medical Sci-ences

چکیده

The present study aimed to synthesize and characterize diopside (CaMgSi₂O₆) and wollastonite (CaSiO₃) nano-bioceramics via a combination of mechano-chemical and calcination processes. In vitro biomineralization and cell responses of wollastonite and diopside were carried out using simulated body fluid (SBF) for up to 28 days and MG-63 osteoblast cells. Results revealed excellent tissue biomineralization of wollastonite and diopside through generating an apatite-like layer on the surface of nano-bioceramics. Wollastonite and diopside cell responses eventuated non-cytotoxicity by MG-63 osteoblast cells, and their viability and cell proliferation were verified. Results of alizarin red staining and alkaline phosphate enzyme of diopside and wollastonite evidenced great bioactivity and tissue biomineralization with respect to the release of Ca²⁺ and high absorption related to calcium activity, and high activity and growth of alkaline phosphate enzyme to repair bone tissue of diopside, and wollastonite was enhanced in contact with the MG-63 osteoblast cells. Regarding the addition of Mg²⁺ into the calcium-silicate network for the chemical stability network to improve biological properties, results of biological assays verified that diopside possessed high biological and cell responses in comparison to wollastonite; and both of them can be suggested as great bioactive and biocompatible candidates for biomedical applications.

کلیدواژه‌ها

Bioactivity؛ Biomineralization؛ Cell responses Diopside؛ Wollastonite

مراجع

[1] A. Borger, P. Supancic, R. Danzer, “The ball on three balls test for strength testing of brittle discs: stress distribution in the disc”, Ceram. Eur. Soc. J., Vol. 22, 2002, pp. 1425-1436.

[2] R. Damani, R. Gstrein, R. Danzer, “Critical notch – root radius effect in SENB – S fracture toughness testing”, Eur. Ceram. Soc. J., Vol. 16, 1996, pp. 695-702.

[3] P.N. De Aza, Z.B. Luklinska, A. Martinez, M.R. Anseau, F. Guitian. S. De Aza, “Morphological and structural study of pseudowollastonite implants in bone”, Microsc. J., Vol. 197, 2000, pp. 60-67.

[4] S.V. Dorozhkin, “Bioceramics of calcium orthophosphates”, Biomat. J., Vol. 31, 2010, pp. 1465-1485.

[5] Q. Fu, E. Saiz, M.N. Rahaman, A.P. Tomsia, “Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives”, Mater. Sci. Eng. C J., Vol. 31, 2011, pp. 1245-1256.

[6] Z. Gou, J. Chang, “Synthesis and in vitro bioactivity of dicalcium silicate powders”, Eur. Ceram. Soc. J., Vol. 24, 2004, pp. 93-99.

[7] C. Wu, Y. Ramaswamy, H. Zreiqat, “Porous diopside (CaMgSi₂O₆) scaffold: A promising bioactive material for bone tissue engineering”, Acta. Biomat. J., Vol. 6, 2010, pp. 2237- 3345.

[8] N. Aza, B. Zofia, M. Anseau, “Bioactivity of diopside ceramic in human”, Biomed. Mater. Res. Part B.: Appl. Biomater. J., Vol. 73, 2005, pp. 54-60.

[9] S. Sadeghzade, R. Emadi, S. Labbaf, “Hardystonite-diopside nanocomposite scaffolds for bone tissue engineering applications”, J. Mat. Chem. and Phy., Vol. 202, 2017,  pp. 95-103.

[10] C. Wu, J. Chang, “A review bioactive silicate ceramics”, J. of Biomed. Mat., Vol.8, 2013. pp.1-12.

[11] A. Najafinezhad, M. Abdellahi, H. Ghayour, A. Soheily, A. Chami, A. Khandan, “A comparative study on the synthesis mechanism, bioactivity and mechanical properties of three silicate bioceramics”, J. Mat. Sci. Eng. C, Vol.72,2017, pp.259-267.

[12] S. Thomas, P.  Balakrishnan, M.S. Sreekala,”Fundamental Biomaterials Ceramics”, Woodhead Publishing Series in Biomaterials, 2018.

[13] C. Wu, J. Chang, “Degaradation bioactivity, and cytocompatibility of diopside, akermanite, and bredigite ceramics”, Biomed. Mat. Res. Part B: Appl. Biomat. J., Vol.83, 2007, pp. 60-153.

[14] C. Wu, J. Chang, J. Wang, S. Ni, W. Zhai, “Preparation and characteristics of a calcium magnesium silicate (bredigite) bioactive ceramic”, Biomat. J., Vol. 26, 2005, pp. 2925-2931.

[15] J.F. Shackelford, R.H.H. Doremus, Handbook ceramic and materials structure, properties and processing, Springer, 2008.

[16] L.H. Long, L.D. Chen, J. Chang, “Low temperature fabrication and characterizaition of –CaSiO₃ ceramics”, Ceram. Int. J., Vol. 32, 2006, pp. 457-460.

[17] P. Palmero, “Structural ceramic nanocomposites, a review of properties and powders synthesis methods”, J. Nanomat., Vol.5, 2015 pp.656-696.

[18] F. Tavangarian, R. Emadi, “Mechanical activation assisted synthesis of pure nanocrystalline forsterite powder”, J. Alloy and Compounds., Vol.485, 2009, pp.648-652.

[19] H. Ghomi, R. Emadi, S. Haghjooyeh Javamard, “Preparation of nanostructure bioactive diopside scaffolds for bone tissue engineering by two near net shape manufacturing techniques”, Mat. Lett. J., Vol. 167, 2016, pp. 157-160.

[20] A. Monshi, M. Foroughi, M. Monshi, “Modifid Scherrer Equation to Estimate More Accurately Nano – Crystallite Size Using XRD”, Nano. Sci. and Eng. J., Vol. 2, 2012, pp. 154-160.

[21] T. Kokubo, H.Takadama, “How useful is SBF in predicting in vivo bone bioactivity?”, Biomat. J., Vol. 27, 2006, pp. 2907-2915.

[22] L. Lu, M.O. Lai, Mechanical Alloying, Kluwer Academic Publisher, Boston, 1998.

[23] G.K. Williamson, W.H. Hall, “X – ray line broadening from filed aluminum and wolfram”, Acta. Metall. J., Vol. 1, 1953, pp. 22-31.

[24] C. Wu, J. Chang, “A review bioactive silicate ceramics”, Biomed. Mat. J., Vol.8, 2013, pp.1-12.

[25] K. Khoshroo, T.S. Jafarzadeh, F. Moztarzadeh, M. Tahriri, H. Jazayeri, L. Tayebi, “Development of 3D PCL microsphere/TiO₂ nanotube composite scaffolds for bone tissue engineering”, Mat. Sci. and Eng. C J., Vol. 70, 2017, pp.586-598