ارتباط برخی از متابولیت‌های خون و بیان ژن فاکتور هسته‌ای رونویسی کاپا B (Nuclear factor kappa B) با کتوز تحت‌بالینی در گاوهای هلشتاین در طول دوره انتقال

آسیب‌شناسی درمانگاهی دامپزشکی

دوره 16، 2 (62) تابستان، شهریور 1401، صفحه 111-126   

نوع مقاله: مقاله علمی پژوهشی

شناسه دیجیتال (DOI): 10.30495/jvcp.2022.1937013.1316

نویسندگان

سعیده مرادی¹؛ غلامعلی مقدم^{* 2}؛ صمد لطف‌اله‌زاده³؛ رضی‌اله جعفری⁴؛ سید‌عباس رافت²

¹دانشجوی دکترای گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

²استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

³دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.

⁴دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.

چکیده

کتوز تحت­بالینی اثرات اقتصادی بر صنعت گاو شیری تحمیل کرده و گاوها با افزایش استرس اکسیداتیو، مستعد ابتلا به این نوع کتوز می­­شوند. از آنجایی‌که فاکتور هسته‌ای رونویسی کاپا B (NF-κB Nuclear factor kappa B;) به‌عنوان فاکتور پاسخ سلولی به انواع استرس با منشأ درونی می‌باشد، در تحقیق حاضر، ارتباط برخی از متابولیت‌های خون و بیان ژن NF-κB با کتوز تحت­بالینی در گاوهای هلشتاین در طول دوره انتقال، در یک گاوداری 2000 رأسی با 900 رأس گاو دوشا بررسی گردید. بدین منظور 5­ میلی‌لیتر خون از 100 رأس گاو شکم چهارم و شکم پنجم زایش، یک هفته قبل و نیز یک هفته بعد از زایش، از ورید دمی اخذ شد. بر اساس یافته­ها، در هفته قبل از زایش همبستگی مثبتی بین اسیدهای چرب غیراستریفیه (NEFAs Non-esterified fatty acids;) و بتاهیدروکسی بوتیرات (Beta-hydroxybutyrate; BHBA)، مالون دی‌آلدئید، گاما گلوتامیل ترانسفراز و NF-κB و نیز همبستگی منفی با لیپوپروتئین با تراکم بالا مشاهده شد. در هفته بعد از زایش هم همبستگی مثبتی بین BHBA، NEFA، مالون­دی‌آلدئید، گاما گلوتامیل­ترانسفراز، آلکالین فسفاتاز و NF-κB و نیز همبستگی منفی با لیپوپروتئین با تراکم بالا مشاهده شد. نقطه برش mM/L 2/1BHBA≤ برای گاوهای سالم و mM/L 2/1BHBA> برای گاوهای مبتلا به کتوز تحت­بالینی در بعد از زایش تعیین شد. همچنین در گاوهای درگیر، مقادیرNEFA ،BHBA ، گاماگلوتامیل ترانسفراز (0001/ (p< و آلکالین فسفاتاز (03/0 p=) افزایش و لیپوپروتئین با تراکم بالا و NF-κB (0001/p<) کاهش داشت. نتایج تحقیق حاضر مشخص کرد که افزایش NEFAs و BHBA با فعال‌سازی مسیر NF-κB و استرس اکسیداتیو، دام را مستعد ابتلا به بیماری کتوز می‌کند.

کلیدواژه‌ها

کتوز تحت‌بالینی؛ استرس‌اکسیداتیو؛ فاکتور هسته‌ای رونویسی کاپا B؛ متابولیت‌های خون؛ گاو هلشتاین

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