Histopathological investigation of the effect of Viola odorata and Pistacia atlantica extract on acute tendonitis in rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

دوره 13، شماره 2، آبان 2022، صفحه 59-65   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.30495/medherb.2022.697141

نویسندگان

Amir Hossein Cheraghian¹؛ Elham Moghtadaiee^{* 2}

¹Graduate of Veterinary Medicine, Shahrekord Branch, Islamic Azad University ,Shahrekord, Iran.

²Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord Branch, , Islamic Azad University , Shahrekord, Iran.

چکیده

Background & Aim: Tendon is the connective tissue between bone and muscle. Because of poor blood flow and the possibility of adhesions during repair, tendon injury management is particularlyimportant.Therefore, finding a quick way to repair tendon tissue is one of the targets that has been pursued over the years.
Experimental: In this study, 24 male rats were divided into 4 groups, including first group without any treatment, second group receiving dexamethasone (1mg/kg), third group receiving 250 mg/kg Viola odorata and Pistacia atlantica extract, fourth group receiving 500 mg/kg and Pistacia atlantica and Viola odorata extract. After anesthesia of rat acute tendonitis developed. Al the mentioned groups were injected subcutaneously on bigining day, 7 and 21 days after surgery and histopathological evaluation was performed on day 21.
Results: The results showed no statistical significance in the number of fibroblasts between different groups. However, dexamethasone groups and the one receiving 500 mg/kg of extract differed significantly from the control group in terms of inflammation and bleeding. Furthermore, the number of fibrocytes in the groups receiving 500 mg/kg and 250 mg/kg doses of Viola odorata and Pistacia atlantica extracts was significantly different from the control and dexamethasone groups (P <0.05).
Recommended applications/industries: According to the results, the amount of collagen and fibrocytes in the group treated with 500 mg/kg had a significant difference, compared to other groups, probably due to the anti-inflammatory, antibacterial and antioxidant properties of Viola odorata and Pistacia atlantica. They can be used in traditional medicine along with other medicines.

کلیدواژه‌ها

Acute tendonitis؛ Histopathology؛ Pistacia atlantica؛ Rat؛ Viola odorata

مراجع

Ahmadi, F., Nobahar, M., Alhani, F. and Falahi-Khoshknab, M. 2011. Factor affecting the quality of ‎nursing care from the retired nursesperspectives. Hjayat, 17(1):24-34.‎

Asnaashari, S., Dastmalchi, S. and Javadzadeh, Y. 2018. Gastroprotective effects of herbal medicines (roots). International Journal of Food Properties, 21(1): 902-920.

 Chen, C. T., Chen, C. H., Sheu, C., Chen, J. P. 2019. Ibuprofen-loaded hyaluronic acid nanofibrous ‎membranes for prevention of postoperative tendon adhesion through reduction of ‎inflammation. International Journal of Molecular Sciences, 20(20): 5038.‎

Darugari, Sh., Parandi, R., Yousef Vand, N. and Shakibaei, D. 2018. Evaluation of analgesic effects of rubbing oil of Pistacia Atlantica using formalin test in male rats. Scientific Journal of Birjand University of Medical Sciences, 24(4): 273-281.

 Huegel, J. B.P. 2020. Effects of pulsed electromagnetic field therapy on rat achilles tendon healing. Journal of Orthopaedic Research, 38(1), 70-81.

Isner-Horobeti, M. E., Dufour, S. P., Vautravers, P., Geny, B., Coudeyre, E. and Richard R. 2013. ‎Eccentric exercise training: modalities, applications and perspectives. Sports Medicine, 43(6): 483-‎‎512.‎

James, R., Kesturu, G., Balian, G. and Chhabra, A.B. 2008. Tendon: biology, biomechanics, ‎repair, growth factors, and evolving treatment options. The Journal of Hand Surgery, 33(1): 102-112.‎

Modaressi, M., Ghrbanali, F. and Sajjadian, A. 2017. Comparison of anticonvulsant effect of violet flower odorata with carbamazepine in animal model of seizures. Armaghane Danesh, 22(4): 431-441.

Mohagheghi, M. and Shirvi, A. 2018. The effect of aqueous extract of violet flower cornuta on testicular damage in streptozotocin-induced male diabetic rats. Neishabour Journal of Medical Sciences, 5(4): 42-51.

Mortazavian, M. and GhorbaniHesari, N. 2012. The effect of violet hydro-alcoholic extract and its fractions on the proliferation of cervical cancer cells. Iranian Journal of Obstetrics, Gynecology and Infertility, 15(22):9-16.

Muqtadaei, E., Izadi Motlagh, M. 2020. The effect of coriander hydroalcoholic extract on testicular tissue and testosterone in male Wistar rats. Animal Physiology and Development, 13: 75-84.

Muslimi, H., Haerian, M., Tehrani Sharif, M. and MazaheriNejad, R. 2020. Histopathological study of Caparis spinosa on the healing process of Achilles tendon injury in rats. Iranian Journal of Veterinary Medicine, 16(2): 60-67.

Naderi, S., Mahmoudvand, H., Mahmoudvand, H., Rashnoo, M. and Khaksarian, M. 2018. Chemical ‎composition, antinociceptive and acute toxicity of Pistacia atlantica fruit extract. Entomology and Applied Science ‎Letters, 5(3): 8-12.‎

Nikniaz, Z., Ostadrahimi, A., Mahdavi, R., Asghar Ebrahimi, A. and Nikniaz, L. 2014. Effects of ‎Elaeagnus angustifolia L. supplementation on serum levels of inflammatory cytokines and matrix ‎metalloproteinases in females with knee osteoarthritis. Complementary Therapies in Medicine, 22(5):‎‎864-869.‎

Oryan, A., Moshiri, A. and Sharifi P. 2012. Advances in injured tendon engineering with emphasis on ‎the role of collagen implants. Hard Tissue, 1(2): 12.

Oryan, A., Moshiri, A. and Sharifi, P. 2013. Advances in injured tendon engineering with emphasis on ‎the role of collagen implants. Hard Tissue, 1(2): 12.

‎Saether, E. E., Chamberlain, C. S., Aktas, E., Leiferman, E. M., Brickson, S. L., Vanderby, R. 2016. ‎Primed mesenchymal stem cells alter and improve rat medial collateral ligament healing. Stem Cell ‎Reviews and Reports, 12(1): 42-53.

Samsam Shariat H. 1995. Growing and the proliferation of medicinal plants. Mani Publications, Esfahan.

Sharma, P. and Maffulli, N. 2008. Tendinopathy and tendon injury: the future. Disability and ‎Rehabilitation, 30(20-22) 1733-1745.‎

Snedeker, J. G., Foolen, J. 2017. Tendon injury and repair–A perspective on the basic mechanisms ‎of tendon disease and future clinical therapy. Acta Biomaterialia, 63: 18-36.‎

Woo, S. L., Mau, J. R., Kang, H., Liang, R., Almarza, A. J., Fisher, M. B. 2019. Functional tissue ‎engineering of ligament and tendon injuries. Principles of Regenerative Medicine, 1179-1198.

Yadegari, M., Parvizi, A. and Moqtadaei Khorasgani, E. 2020. Evaluation of the effects of general trancron injection on the tendon repair process in rabbits by ultrasonography and histopathology. Scientific-Research Journal of Sabzevar University of Medical Sciences, 27(3):483-491.

‎Yang, M., Wang, Z., Li, Y. and Guo, B. 2013. Bilateral cadaveric Achilles tendon graft in ‎reconstruction after Achilles tendon tumor resection. The Journal of Foot and Ankle Surgery, 52(1):‎‎103-106.