The effect of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)

International Journal of Biophotonics and Biomedical Engineering

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 07 دی 1401

نوع مقاله: Research article

شناسه دیجیتال (DOI): 10.30495/ijbbo.2022.1964975.1014

نویسندگان

habib tajalli^{* 1}؛ Masoud Maleki²؛ Esmail Safavi³؛ Reza Shahi⁴؛ Fatemeh Firoozi²؛ Zahra Akbarpour²؛ Ali reza Sotoudeh Khyaban⁵

¹Biophotonics Research center

²Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran

³Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Azad Islamic University, Tabriz, Iran

⁴Department of Mathematics and Statistics, Tabriz branch, Islamic Azad University, Tabriz, Iran

⁵Research Center of Biophotonics, Islamic Azad University, Tabriz Branch, Tabriz, Iran

چکیده

This research aimed to investigate the effect of low power laser on spermatogenesis in testicular tissue of azoospermia mouse model in-vivo. In this experimental work, 112 adult male Syrian mice were randomly divided into three groups (harboring 8 mice each): negative control, positive (Azoospermia control) group, and experimental group. To create azoospermia in positive animals, Busulfan was used at a dose of 30mg/kg, for 21 days by intraperitoneal injection. In the experimental group after Busulfan treatment, they were affected by low power diode laser (wavelength of 808nm) at three different energy densities of 2, 4, and 8 J/cm2. The employment of a laser with an energy density of 8 J/cm2 was shown to be beneficial in boosting germ cell and sperm production.

کلیدواژه‌ها

spermatogenesis؛ azoospermia؛ laser therapy؛ low power laser؛ infertility؛ Busulfan