Selection of native lactic acid bacteria with probiotic potential from Nile tilapia (Oreochromis niloticus) gastrointestinal tract

International Aquatic Research

مقاله 4، دوره 15، شماره 2، شهریور 2023، صفحه 135-144   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2023.1973419.1362

نویسندگان

Ana Antonia Zapata¹؛ Ruth López-Alcántara²؛ Myrna Leticia Bravo-Olivas³؛ Rosa Chávez-Dagostino³؛ Julia Ramos-Miranda⁴؛ David Ulises Miranda-Hernández⁵؛ Maurilio Lara-Flores^{* 6}

¹BEMAREMA´s Doctorate, Coast University Centre from Guadalajara University. Puerto Vallarta, Jalisco, Mexico

²Biomedical Research Centre, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico

³Biological Science Department, Coast University Centre from Guadalajara University. Puerto Vallarta, Jalisco, Mexico

⁴Ecology and Fisheries Laboratory, EPOMEX Institute, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico

⁵Agricultural Science Faculty, Autonomous Campeche University, Escarcega, Campeche, Mexico

⁶Molecular Microbiology Laboratory, EPOMEX Institute, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico

چکیده

The main objective was to select and identify native lactic acid bacteria from the gastrointestinal tract of Nile tilapia (Oreochromis niloticus) to use as a possible probiotic. Seventeen Lactic Acid Bacteria (LAB) were isolated from the gastrointestinal tract of juvenile Nile tilapia. Six strains were selected and named in the first phenotypic and morphological selection process: C3, C4, C7, C15, C16, and C25. The strains C3, C7, and C25 presented high antagonism activity towards pathogens strain, high survival index against bile salts and acidity tolerances, optimum acid lactic production, and enzymatic activity for carbohydrates and protein substrate. Therefore, strains C3, C7, and C16 presented potential probiotics for Nile tilapia aquaculture. The biological effects of the strains in Nile tilapia will study in a future experiment where the strains will include in fish feed.

کلیدواژه‌ها

Probiotics؛ Lactic acid bacteria؛ Nile tilapia؛ Oreochromis niloticus