Prevalence and antibiotic resistance pattern of Campylobacter species isolated from raw cow milk in Urmia, Iran

Journal of Basic and Clinical Veterinary Medicine

دوره 4، شماره 1، آذر 2023، صفحه 29-38   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/jbcvm.2023.1979879.1039

نویسندگان

Maryam Mostafavi¹؛ Moslem Neyriz-Naghadehi^{* 2}

¹Department of Biology, Faculty of Basic Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran

²Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

چکیده

Campylobacter species are the most common bacterial cause of diarrhea in humans. The present study investigated the prevalence and antibiotic resistance of Campylobacter spp. isolated from raw milk in Urmia, Iran. Eighty raw cow milk samples were randomly collected from traditional dairy retailers in different regions of Urmia by sterile conditions in 2018. First, the samples were enriched in supplemented Preston broth, and then they were streaked onto supplemented Campylobacter agar and incubated at 42 °C for 48 h in microaerophilic conditions. Biochemical tests, such as hippurate hydrolysis and susceptibility or resistance to nalidixic acid, were performed to identify the species of isolates. Antibiotic sensitivity test on isolates was performed by the Kirby-Bauer disc diffusion method. Thirteen samples (16.25%) were contaminated with Campylobacter spp. The prevalence of Campylobacter jejuni (13.75%) was higher than Campylobacter coli isolates (2.5%). The Campylobacter isolates showed high resistance against tetracycline (100%), co-trimoxazole (84%), ampicillin, ceftriaxone, and chloramphenicol (69.2%) while exhibiting moderate resistance to ciprofloxacin and nitrofurantoin (46.2%) and low resistance to gentamicin (30.8%). Moreover, nine isolates (69.2%) showed multi-drug resistance (MDR). It can be concluded the prevalence of Campylobacter spp. and their MDR strains in distributed raw cow milk in Urmia is high. It is recommended to improve animal health and milk hygiene, prevent the overuse of antibiotics in dairy farms, and pasteurize milk.
Keywords: Raw milk, Campylobacter species, Antibiotic resistance pattern, Urmia, Iran

کلیدواژه‌ها

Raw cow milk؛ Campylobacter species؛ Antibiotic resistance pattern؛ Urmia؛ Iran