بررسی فیتوشیمیایی، ضدباکتریایی و ضدقارچی اسانس دو گونه از گیاه دارویی (Thymus daenensis و Thymus fedtschenkoi) در غرب ایران

اکوفیتوشیمی گیاهان دارویی

مقاله 1، دوره 11، شماره 1، فروردین 1402، صفحه 1-16   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/ejmp.2022.1954831.1683

نویسندگان

حسن مومیوند^{* 1}؛ پریسا خانی زاده¹؛ حمید حسنیان خوشرو²

¹گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

²استادیار، سازمان تحقیقات، آموزش و ترویج کشاورزی، موسسه تحقیقات کشاورزی دیم کشور، مراغه، ایران

چکیده

در این تحقیق به منظور بررسی فیتوشیمیایی، ضدباکتریایی و ضدقارچی دو گونه از آویشن دنایی (Thymus daenensis Celak.) و آویشن قره‌باغی (Thymus fedtschenkoi Ronniger .) علیه باکتری‌های گرم مثبت (Bacillus cereus و Staphylococcus aureus  وEnterococcus faecium)، باکتری‌های گرم منفی (Escherichia coli و Salmonella enteritidis) و قارچ (Candida albicans) ، سرشاخه های گلدار گیاهان به‌ترتیب از اراک و همدان، در مرحله گلدهی کامل  برداشت شد. اسانس گیری به روش تقطیر با آب (طرح کلونجر) و شناسایی ترکیبات اسانس‌ها توسط کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS)انجام گردید. بررسی ضد میکروبی با استفاده از روش های دیسک دیفیوژن ، میکرودایلوشن ، حداقل غلظت مهارکنندگی (MIC) و حداقل غلظت کشندگی باکتری (MBC) محاسبه گردید. نتایج نشان داد محتوی اسانس آویشن دنایی (3/4 درصد) بیشتر از آویشن قره‌باغی (2/9 درصد) بود. لینالول (83/1 درصد) و تیمول (73/9 درصد) به ترتیب مهمترین ترکیبات آویشن قره‌باغی و آویشن دنایی را تشکیل دادند و اینکه  اسانس هر دو گونه دارای اثر ضدباکتریایی و ضدقارچی قابل توجهی بودند؛ به‌طور کلی فعالیت ضدباکتریایی اسانس آویشن قره‌باغی به‌ویژه در مورد باکتری‌های گرم مثبت بیشتر از اسانس آویشن دنایی بود. درحالی­که فعالیت ضدباکتریای اسانس آویشن دنایی بر اشرشیاکلی (گرم منفی) بیشتر از اسانس آویشن قره‌باغی بود. فعالیت ضدقارچی اسانس آویشن دنایی در مقایسه با آویشن قره‌باغی بر قارچ کاندیدیا آلبیکنس نیز بیشتر بود. ترکیبات لینالول و تیمول از مهمترین ترکیبات با اثر ضدمیکروبی در هر دو گونه گزارش شده است.

کلیدواژه‌ها

آنتی‌باکتریال؛ آویشن؛ اسانس؛ تیمول؛ لینالول

مراجع

Al-Fattani, M.A. and Douglas, L.J. (2006). Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. J Med Microbiol. 55,  999-1008.

Alsaraf, S., Hadi, Z., Al-Lawati, W.M., Al-Lawati, A.A. and Khan, S.A.  (2019). Chemical composition, in vitro antibacterial and antioxidant potential of Omani Thyme essential Oil along with in silico studies of its major constituent. Journal of King Saud University – Science. doi: https://doi.org/10.1016/j.jksus.2019.09.006

Ahmadi, E., Abdollahi, A., Najafipour, S., Meshkibaf, M.H., Fasihi Ramandi, M. and Namdar, N. (2016). Surveying the effect of the phenol compounds on antibacterial activity of herbal extracts: In vitro assessment of herbal extracts in Fasa-Fars province. J Fasa Univ Med Sci. 6, 210-20.

Afonso, A.F., Pereira, O.R., Valega, M., Silva, A.M.S, and Cardoso, S.M. (2018). Metabolites and biological activities of Thymus zygis, Thymus pulegioides, and Thymus fragrantissimus grown under organic cultivation. Molecules. 23, 1–15.

Adams, R.P. (2007). Identification of essential oils components by gas chromatography/quadrupole mass spectroscopy, 4^th ed. Allured Publishing Corporation, Carol Stream, IL, USA. p. 804.

Alves, M., Gonçalves, M.J., Zuzarte, M., Alves-Silva, J.M., Cavaleiro, C., Cruz, M.T. and Salgueiro, L. (2019). Unveiling the antifungal potential of two Iberian thyme essential oils: effect on C. albicans germ tube and preformed biofilms. Front. Pharmacol. 10:446. doi: 10.3389/fphar.2019.00446

Anzlovar, S., Baricevic, D., Avgustin, J.A. and Koce, J.D. (2014). Essential oil of common Thyme as a natural antimicrobial. Food Additive. Food Technol. Biotechnol. 52, 263–268

Amiri, H. (2012). Essential oil composition and antioxidant properties of three Thymus species. Evidence-Based Complementary and Alternative Medicine, 1-8.

Bagamboula, C.F., Uyttendaele, M. and Debevere, J. (2004). Inhibitory effect of thyme and Basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiology. 21, 33–42.

Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods. International Journal of Food Microbiology. 94, 223–253.

Bhavaniramya, S., Vanajothi, R., Vishnupriya, S., Al-Aboody, M. S., & Baskaran, D. (2019). Computational characterization of deleterious SNPs in Toll-like receptor gene that potentially cause mastitis in dairy cattle. Biocatalysis & Agricultural Biotechnology. 19, 101-151

Calo, J.R., Crandall, P.G., O’Bryan, C.A. and Ricke, S.C. (2015). Essential oils as antimicrobials in food systems –A review. Food Control. 54,111–119.

Dorri, M.A., Mirza, M. and Sharifi Ashoorabadi, E. (2021). Phytochemical study of essential oil in different extensions of Thymus daenensis Celak. cultivated in lowland lands: A case study in Golestan province. Eco-phytochemical Journal of Medicinal Plants. 9th Year, Volume One. 90-99.

El-Mohamedy, R.S.R. (2017). Plant essential oils for controlling plant pathogenic fungi. 171-198. In: Volatiles and Food Security: Role of Volatiles in Agro-ecosystems. Choudhary D.K. (eds.). Springer Singapore, 373 p.

Ghasemi Pirbalouti, A., Neshat, S.H., Rahimi, E., Hamedi, B. and F, Malekpoor. (2013). Chemical composition and antibacterial activity of essentials oils of Iranian herbs against Staphylococus aureus isolated from milk. International Journal of Food Properties. 17:9, 2063-2071.

Ghasemi Pirbalouti, A., Bahmani, M. and Avijgan, M. 2009. Anti- Candida activity of Iranian medicinal plants. Electronic Journal of Biology. 5, 85-88

Ghasemi Pirbalouti, A., Malekpoor, F., Enteshari, S., Yousefi, M., Momtaz, H. and Hamedi, B. 2010a. Antibacterial activity of some folklore medicinal plants used by Bakhtiari tribal in Southwest Iran. International Journal of Biology, 2, 55-63.

Ghasemi Pirbalouti, A., Jahanbazi, P., Enteshari, S., Malekpoor, F. and Hamedi, B. 2010b. Antimicrobial activity of some of the Iranian medicinal plants. Archives of Biological Science Belgrade. 62, 633- 642.

Ghasemi Pirbalouti, A., Emami Bistghani1, Z. and Malekpoor, F. 2015. An overview on genus Thymus. Journal of Herbal Drugs. Vol. 6, 93-100.

Ghelichnia, H. 2016. Essential oil composition of tree species of Thymus growing wild in mazandaran, iran. Cercetări Agronomice în Moldova Vol. XLIX , No. 2 , 107-113.

Golparvar, A., Ghasemi Pirbalouti, A., Zinali, H. and Hadipanah, A. (2012). Effect of harvest times on quality (morphological) and quality characteristics of Thymus daenensis celak in Isfahan. Journal of Herbal Drugs. Vol. 2, 245-254,

Gualco, L., Debbia, E.A., Bandettini, R., Pescetto, L.,  Cavallero, A. and Ossi, M.C. (2007). Antifungal resistance in Candida spp. isolated in Italy between 2002 and 2005 from children and adults. International Journal of Antimicrobial Agents. 29,179-84.

Guo, F., Liang, Q., Zhang, M., Chen, W., Chen, H., Yun, Y., Zhong, Q. and Chen, W. (2021). Antibacterial activity and mechanism of linalool against Shewanella putrefaciens. Molecules. 26, 245.

Hadian, J., Akramian, M., Heydari, H., Mumivand, H. and Asghari, B. (2012). Composition and in vitro antibacterial activity of essential oils from four Satureja species growing in Iran. Natural Product Research. 26, 98-108.

Hadipanah, A. and Khorami, M. (2016). Chemical composition and antibacterial activity of essential oil from two Thymus species. Journal of Herbal Drugs. Vol. 6, 211-217

Jawetz, E., Melnick, J.L. and Adelberg, E.A. (2013). Medizinische Mikrobiologie. Berlin: Springer-Verlag

Han, X., Parker, T.L. (2017). Antiinflammatory activity of cinnamon (Cinnamomum zeylanicum) bark essential oil in a human skin disease model. Phytother Res. 31, 1034–8.

Hadian, J., Farzaneh, M., Fakhr Tabatabaei, S.M., Mirjalili, M.H., Ranjbar, H. and Haji Eghrari, B. (2008). A study of the composition, and antifungal activity of the essential oils of Artemisia scopari and A. aucheri from south of Khorasan on some soil-borne phytopathogen. Iranian Journal of Agriculture Science. 38, 421-429.

Kalvandi, R., Atri, M., Jamzad, Z. and Safikhani, K. (2012). Taxonomic study of Thymus eriocalyx (Ronniger) Jalas in Iran with emphasis on floristic marker and using special station method. Taxonomy and Biosystematics. 10, 63-76.

Khoshsokhan, F., Babalar, M., Pourmeidani, A. and Fatahi, M. (2015). Antioxidant activity, total phenolics and oil content of some Thymus kotschyanus and Thymus daenensis populations. Plant Production Technology. 15, 153-162.

Khorshidi, J., Rasouli, M., Rustaiee, A.R. and Mohamadparast, B. (2014).Chemical composition of the essential oil of Thymus fedtschenkoi growing wild in Iran, Journal of Essential Oil Bearing Plants. 17, 173-175.

Mahboubi, M., Heidarytabar, R., Mahdizadeh, E. and Hossein, H. (2017). Antimicrobial activity and chemical composition of Thymus species and Zataria multiflora essential oils. Agriculture and Natural Resources. 51, 395-401.

Morshedloo, M. R., Amani Machiani, M., Mohammadi, A., Maggi, F., Aghdam, M. S., Mumivand, H., & Javanmard, A. (2021). Comparison of drying methods for the extraction of essential oil from dragonhead (Dracocephalum moldavica L., Lamiaceae). Journal of Essential Oil Research, 33(2), 162-170.

Mumivand, H., Ebrahimi, A., Morshedloo, M.R. and Shayganfar, A. (2021). Water deficit stress changes in drug yield, antioxidant enzymes activity and essential oil quality and quantity of Tarragon (Artemisia dracunculus L.). Industrial Crops and Products. 164, 113-381.

Mumivand, H., Aghemiri, A., Aghemiri, A., Morshedloo, M. R. and Nikoumanesh, K. (2019). Ferulago angulata and Tetrataenium lasiopetalum: essential oils composition and antibacterial activity of the oils and extracts. Biocatalysis and Agricultural Biotechnology. 22, 101-407.

Mumivand, H., Babalar, M., Hadian, J. and Tabatabaei, S.M.F. (2010). Influence of nitrogen and calcium carbonate application rates on the minerals content of summer savory (Satureja hortensis L.) leaves. Horticulture, Environment, and Biotechnology. 51, 173-177.

Najib Zade, T., Yadegari, M.H. and Naghdi Badi, H. (2009). Evaluation antifungal effects of essential oils Satureja khuzestanica and Myrtus communis. Presented for the M.Sc, Tarbiat Modares university, Tehran.

Nejad Ebrahimi, S., Hadian, J., Mirjalili, M.H., Sonboli, A. and Yousefzadi, M. (2008). Essential oil composition and antibacterial activity of Thymus caramanicus at different phonological stages. Food Chemistry. 110, 927-931.

Nooshkam, A., Mumivand, H., Hadian, J., Alemardan, A. and Morshedloo, M.R. (2017). Drug yield and essential oil and carvacrol contents of two species of Satureja (Satureja  khuzistanica Jamzad and Satureja  rechingeri Jamzad) cultivated in two different locations. Journal of Applied Research on Medicinal and Aromatic Plants. 6, 126-130.

Nagoor Meeran MF, Javed H, Al Taee H, Azimullah Sh, Ojha SK. (2019).  Pharmacological properties and molecular mechanisms of thymol: Prospects for its therapeutic potential and pharmaceutical development. Frontiers in Pharmacology. 8, 380

Nickavar, B., Mojab, F. and Dolat-Abadi., R. (2005). Analysis of the essential oils of two Thymus species from Iran. Food Chem. 90, 609–611.

Pattnaick, S., Subramanyam, V.R., Bapaji, M. and Kole, C.R. (1997). Antibacterial and antifungal activity of aromatic constituents of essential oils. Microbios. 89, 39–46.

Pavela, R., Maggi, F., Kamte, S.N., Rakotosaona, R., Rasoanaivo, P., Nicoletti, M. and Benelli, G. (2017). Chemical composition of Cinnamosma madagascariensis (Cannelaceae) essential oil and its larvicidal potential against the filariasis vector Culex quinquefasciatus Say. South African Journal of Botany. 108, 359-363.

 Pavela, R., Morshedloo, M.R., Mumivand, H., Khorsand, G.J., Karami, A., Maggi, F. and Benelli, G. (2020). Phenolic monoterpene-rich essential oils from Apiaceae and Lamiaceae species: insecticidal activity and safety evaluation on non-target earthworms. Entomologia Generalis. 421-435.

Perumal, P., Mekala, S. and Chaffin, W.L. (2007). Role for cell density in antifungal drug resistance in Candida albicans biofilms. Antimicrob Agents Chemother. 51, 2454–63.

Pandey, A.K., Kumar, P., Singh, P., Tripathi, N.N. and Bajpai, V.K. 2017. Essential oils: sources of antimicrobials and food preservatives. Frontiers in microbiology. 7, 21-61.

Rahmati, P., Noorbakhsh, F., Pazoki ., A. (2020). Effect of Zataria Multiflora and Cinnamomum Verum essential oils on biofilm formation of Staphylococcus aureus. Applied Biology. Volume 10, Issue 39, P 37-54

Rustaiee, A., Mirahmadi, S., Sefidkon, F., Fakhr Tabatabaei, M. and Omidbaigi, R. (2011). Essential oil content and composition of Thymus fedtschenkoi Ronniger at different phenological stages. Journal of Essential Oil Bearing Plants. 14, 625-629.

Ranjbar, H., Farzaneh, M., Hadian, J., Mirjalili, M.H. and Sharifii, R. (2008). Antifungal effect of several herbal essential oils on post-harvest disease of fruit strawberry. Pajouhesh & Sazandegi. 21, 54-60

Safaei, L., Sharifi Ashoorabadi, E. and Afuni, D. (2017). Effect of application of chemical fertilizers, livestock and their integration on the performance of compounds phytochemical essential oil of medicinal herbs Thymus daenensis Celak. Eco-phytochemical Journal of Medicinal Plants. 5, 13-24.

Seneviratne, C.J., Jin, L.J., Samaranayake, Y.H. and Samaranayake, L.P. (2008). Cell density and cell aging as factors modulating antifungal resistance of Candida albicans biofilms. Antimicrob agents chemother. 52, 3259-66.

Sengupta. S., Chattopadhyay, M.K. and Grossart, H.P. (2013). The multifaceted roles of antibiotics and antibiotic resistance in nature. Front Microbiol. 4, 47-52.

Taftian, m. Lotfali, e. Farhadi, s. and Jolehar, j. (2020). Evaluation of antifungal effects of aqueous extract of Mentha Longifolia using 0.2% chlorhexidine on clinical isolates of Candida Albicans from Oral Cavity of Patients with Leukemia. The Quarterly journal of School of medicine, Shahid Beheshti University of Medical Sciences. 44, No.3

Teixeira, B.,  Marques, A.,  Ramos, C., Neng,  N.R. and  Nogueira. J.M.F. (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops and Products. 43, 587– 595.

Wright, G.D. (2014). Something new: Revisiting natural products in antibiotic drug discovery. Can J Microbiol. 60, 147-54.

Xiao, s., Cui, p., Shi, w and Zhang, y. (2020). Identification of essential oils with activity against stationary phase Staphylococcus aureus. BMC Complementary Medicine and Therapies. P 1-10. https://doi.org/10.1186/s12906-020-02898-4

Zhang, L.L., Zhang, L.F., Hu, Q.P., Hao, D.L. and Xu, J.G. (2017). Chemical composition, antibacterial activity of Cyperus rotundus rhizomes essential oil against Staphylococcus aureus via membrane disruption and apoptosis pathway. Food Control. 80, 290–296.

Zhang, Y., Liu, X., Wang, Y., Jiang, P. and Quek, S. (2016). Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control. 59, 282–289.

Zhu, y., Li, c., Cui, h., Lin, l. (2021). Encapsulation strategies to enhance the antibacterial properties of essential oils in food system. A Review. Food Control. 123, 107-856.