اثرات اندوفیت‌های آویشن (Thyme) لردگان روی باکتری شیگلا سونئی و قارچ کاندیدا آلبیکنس

بهداشت مواد غذایی

مقاله 2، دوره 12، 4 (48) زمستان، اسفند 1401، صفحه 17-29   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/jfh.2023.1978116.1389

نویسندگان

سمیه شاهرخ شهرکی^{* 1}؛ محمد خدادادی²؛ اعظم مختاری¹

¹گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران

²گروه پاتوبیولوژی دانشکده دامپزشکی دانشگاه شهرکرد، شهرکرد، ایران

چکیده

این پژوهش با هدف بررسی اثرات اندوفیت‌های گیاه دارویی آویشن لردگان بر باکتری شیگلا سونئی و قارچ کاندیدا آلبیکنس طراحی و انجام گردید. اجزای گیاه آویشن بعد از جمع‌آوری و انتقال به آزمایشگاه به ترتیب در اتانول ۷0 درصد (۲ دقیقه)، هیپوکلریت سدیم ۵/۳ درصد (۵دقیقه) و اتانول ۷5 درصد (30 ثانیه) غوطه‌ور و توسط آب مقطر استریل شستشو داده شد. سپس آنها را بر روی محیط YEA و PA کشت داده و اندوفیت‌ها جدا گشتند. از قسمت‌های مختلف گیاه آویشن لردگان (ساقه، برگ و ریشه) در مجموع 8 اندوفیت باکتریایی جدا شده و مورد بررسی قرار گرفت. اندوفیت‌های جدا شده از گیاه آویشن از نوع باسیل، کوکوباسیل و کوکسی بودند. خواص ضد‌میکروبی و مهاری اندوفیت‌های جدا شده از گیاه آویشن لردگان در دو روش‌ بررسی عوامل ساختاری و روش بررسی متابولیت‌های ترشحی اندوفیت‌ها مورد مطالعه قرار گرفتند. نتایجی که از این پژوهش حاصل گردید نشان‌دهنده تأثیرات مطلوب اندوفیت‌های گیاه آویشن لردگان بر باکتری شیگلا سونئی و قارچ کاندیدا آلبیکنس بوده است. در بررسی عوامل ساختاری، اندوفیت‌های جدا شده از ریشه، ساقه و برگ آویشن لردگان، اثرات مهاری قوی‌تری نسبت به متابولیت‌های ترشحی اندوفیت‌های باکتریایی آویشن برعلیه باکتری شیگلا سونئی و قارچ نشانگر کاندیدا آلبیکنس از خود نشان دادند. بطور کلی گیاه دارویی آویشن جایگزین مناسبی برای داروهای شیمیایی در درمان عفونت‌های کاندیدایی به ویژه کاندیدیازیس جلدی مخاطی و شیگلوزیس بوده و می‌تواند در موارد کلینیکی و درمانی، صنایع غذایی، بهداشتی و داروسازی به کار گرفته شود.

کلیدواژه‌ها

اندوفیت؛ آویشن لردگان؛ شیگلا سونئی؛ کاندیدا آلبیکنس

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