تاثیر یک دوره تمرین هوازی همراه با مکمل استویا روی هپاتوکاین‌ها و مقاومت به انسولین در موش‌های دیابتی نوع یک

فصلنامه زیست شناسی جانوری

دوره 15، شماره 4، شهریور 1402، صفحه 273-293   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22034/ascij.2023.1979747.1464

نویسندگان

اسماعیل پیری؛ اکبر نوری حبشی^* ؛ محمد رضا ذولفقاری دیدنی

گروه فیزیولوژی ورزشی و حرکات اصلاحی، دانشگده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

چکیده

دیابت یکی از دلایل اصلی مرگ و میر در کشورهای در حال توسعه است. از راه­های پیشگیری از دیابت و جلوگیری از عوارض ناشی از آن، انجام فعالیت بدنی و استفاده از مکمل­های آنتی اکسیدانی است. هدف از پژوهش حاضر بررسی تاثیر یک دوره تمرین هوازی و مکمل استویا روی فتوئین b، پنتراکسین ۳ و GDF-15 پلاسما و مقاومت به انسولین در موش‌های دیابتی نوع 1 بود. برای این منظور 25 موش دیابتی نوع یک به 5 گروه کنترل-سالم، کنترل دیابت، دیابت مکمل، دیابت تمرین و دیابت تمرین-مکمل تقسیم شدند.  برنامه تمرینی رت­ها برای مدت 65 روز، با سرعت 20 تا 30 متر بر دقیقه با شدت 10 تا 25  متر/دقیقه بود. دوز مصرفی استویا 250 میلی­گرم/کیلوگرم وزن بدن به صورت گاواژ دریافت شد. کشتار موش‌ها 48 ساعت بعد از آخرین جلسه تمرینی انجام و خونگیری انجام شد. جهت مقایسه بین گروه‌ها از آزمون آنالیز واریانس یک­طرفه و آزمون تعقیبی بونفرونی استفاده شد. بررسی تغییرات بین گروهی نشان داد که تفاوت معنی دار بین گروه‌ها برای فتوئین b، پنتراکسین ۳ و GDF-15 پلاسما وجود دارد (001/0p <). بررسی تغییرات بین کروهی نشان داد که فتوئین B و GDF-15 افزایش معنی دار و پنتراکسین کاهش معنی دار در گروه سالم نسبت به گروه دیابتی داشتند  (001/0p <). همچنین بین گروه کنترل دیابت با گروه های تمرین مکمل و تمرین تفاوت معنی دار بین همه شاخص­ها وجود داشت (001/0p <). نتایج پژوهش حاضر نشان داد که تمرین هوازی و مکمل استویا میتوانند باعث کاهش مقاومت به انسولین، فتوئین b و GDF-15 و افزایش پنتراکسین ۳ در موش‌های دیابتی نوع 1 شوند. این بهبودها در گروهی که تمرین و مکمل را به طور همزمان مصرف کرده بودند، بیشتر بود.

کلیدواژه‌ها

استویا؛ تمرین هوازی؛ دیابت نوع 1؛ مقاومت به انسولین

مراجع

1. Adela R., Banerjee S.K. 2015. GDF-15 as a target and biomarker for diabetes and cardiovascular diseases: a translational prospective. Journal of Diabetes Research, 2015.
2. Akbari, A., Tadibi, V., Behpour, N. 2019. The effect of Stevia extract consumption and aerobic eExercise on serum omentin-1 and lipid profile in STZ induced diabetic rats. Journal of Sport Biosciences, 11(2):179-194.
3. Akbarzadeh, S., Eskandari, F., Tangestani, H., Bagherinejad, S.T., Bargahi, A., Bazzi, P., Rahbar, A.R. 2015. The effect of Stevia rebaudiana on serum omentin and visfatin level in STZ-induced diabetic rats. Journal of Dietary Dupplements, 12(1):11-22.
4. Almarashda, O., Abdi, S., Yakout, S., Khattak, M.N.K., Al-Daghri, N.M. 2022. Hepatokines Fetuin-A and Fetuin-B status in obese Saudi patient with diabetes mellitus type 2. American Journal of Translational Research, 14(5):3292.
5. Assaei, R., Mokarram, P., Dastghaib, S., Darbandi, S., Darbandi, M., Zal, F., Omrani, G.H.R. 2016. Hypoglycemic effect of aquatic extract of Stevia in pancreas of diabetic rats: PPARγ-dependent regulation or antioxidant potential. Avicenna Journal of Medical Biotechnology, 8(2):65.
6. Bootcov, M.R., Bauskin, A.R., Valenzuela, S. M., Moore, A. G., Bansal, M., He, X.Y., Pryor, K. 1997. MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-β superfamily. Proceedings of the National Academy of Sciences, 94(21):11514-11519.
7. Boulogne, M., Sadoune, M., Launay, J., Baudet, M., Cohen-Solal, A., Logeart, D. 2017. Inflammation versus mechanical stretch biomarkers over time in acutely decompensated heart failure with reduced ejection fraction. International Journal of Cardiology, 226:53-59.
8. Cai, L., Li, C., Wang, Y., Mo, Y., Yin, J., Ma, X. 2021. Increased serum GDF15 related to improvement in metabolism by lifestyle intervention among young overweight and obese adults. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 14:1195.
9. Carrera-Lanestosa, A., Coral-Martínez, T., Ruíz-Ciau, D., Moguel-Ordoñez, Y., Segura-Campos, M.R. 2020. Phenolic compounds and major steviol glucosides by HPLC-DAD-RP and invitro evaluation of the biological activity of aqueous and ethanolic extracts of leaves and stems: S. rebaudiana Bertoni (creole variety INIFAP C01) S. rebaudiana Bertoni (creole variety INIFAP C01): Bioactive compounds and Functionality. International Journal of Food Properties, 23(1):199-212.
10. Chen, T.H., Chen, S.C., Chan, P., Chu, Y.L., Yang, H.Y., Cheng, J.T. 2005. Mechanism of the hypoglycemic effect of stevioside, a glycoside of Stevia rebaudiana. Planta Medica, 71(02):108-113.
11. Chrysovergis, K., Wang, X., Kosak, J., Lee, S.H., Kim, J.S., Foley, J.F., Eling, T.E. 2014. NAG-1/GDF-15 prevents obesity by increasing thermogenesis, lipolysis and oxidative metabolism. International Journal of Obesity, 38(12):1555-1564.
12. Chu, S.H., Park, J.-H., Lee, M.K., Jekal, Y., Ahn, K.Y., Chung, J.Y., Im, J.-A. 2012. The association between pentraxin 3 and insulin resistance in obese children at baseline and after physical activity intervention. Clinica Chimica Acta, 413(19-20):1430-1437.
13. De Luca, C., Olefsky, J.M. 2008. Inflammation and insulin resistance. FEBS Letters, 582(1):97-105.
14. Dostálová, I., Roubíček, T., Bártlová, M., Mráz, M., Lacinová, Z., Haluzíková, D., Haluzík, M. 2009. Increased serum concentrations of macrophage inhibitory cytokine-1 in patients with obesity and type 2 diabetes mellitus: the influence of very low calorie diet. European Journal of Endocrinology, 161(3):397-404.
15. Dridi, R., Dridi, N., Govindasamy, K., Gmada, N., Aouadi, R., Guénard, H., Hackney, A.C. 2021. Effects of Endurance Training Intensity on Pulmonary Diffusing Capacity at Rest and after Maximal Aerobic Exercise in Young Athletes. International Journal of Environmental Research and Public Health, 18(23):12359.
16. Fairlie, W.D., Zhang, H.P., Brown, P. K., Russell, P. K., Bauskin, A.R., Breit, S.N. 2000. Expression of a TGF-β superfamily protein, macrophage inhibitory cytokine-1, in the yeast Pichia pastoris. Gene, 254(1-2):67-76.
17. Galliera, E., Lombardi, G., Marazzi, M. G., Grasso, D., Vianello, E., Pozzoni, R., Corsi Romanelli, M.M. 2014. Acute exercise in elite rugby players increases the circulating level of the cardiovascular biomarker GDF-15. Scandinavian Journal of Clinical and Laboratory Investigation, 74(6):492-499.
18. Gregersen, S., Jeppesen, P. B., Holst, J. J., Hermansen, K. 2004. Antihyperglycemic effects of stevioside in type 2 diabetic subjects. Metabolism, 53(1):73-76.
19. Guo, H., Qiu, X., Deis, J., Lin, T.Y., Chen, X. 2020. Pentraxin 3 deficiency exacerbates lipopolysaccharide-induced inflammation in adipose tissue. International Journal of Obesity, 44(2): 525-538.
20. Guo, V.Y., Cao, B., Cai, C., Cheng, K. K.Y., Cheung, B.M.Y. 2018. Fetuin-A levels and risk of type 2 diabetes mellitus: a systematic review and meta-analysis. Acta Diabetologica, 55(1):87-98.
21. Hussein, A.M., Eid, E.A., Bin-Jaliah, I., Taha, M., Lashin, L.S. 2020. Exercise and Stevia rebaudiana (R) extracts attenuate diabetic cardiomyopathy in type 2 diabetic rats: possible underlying mechanisms. Endocr Metab Immune Disord Drug Targets, 20:1117-1132.
22. Ivy J.L. 1997. Role of exercise training in the prevention and treatment of insulin resistance and non-insulin-dependent diabetes mellitus. Sports Medicine, 24(5): 321-336.
23. Jan, S. A., Habib, N., Shinwari, Z. K., Ali, M., Ali, N. 2021. The anti-diabetic activities of natural sweetener plant Stevia: an updated review. SN Applied Sciences, 3(4):1-6.
24. Karakas, M. F., Buyukkaya, E., Kurt, M., Motor, S., Akcay, A. B., Karakas, E., Sen, N. 2013. Serum pentraxin-3 levels are associated with the severity of metabolic syndrome. Medical Principles and Practice, 22(3):274-279.
25. Kleinert, M., Clemmensen, C., Sjøberg, K. A., Carl, C.S., Jeppesen, J.F., Wojtaszewski, J.F., Richter, E.A. 2018. Exercise increases circulating GDF15 in humans. Molecular metabolism, 9:187-191.
26. Kolahdouzi, S., Baghadam, M., Kani-Golzar, F.A., Saeidi, A., Jabbour, G., Ayadi, A., Zouhal, H. 2019. Progressive circuit resistance training improves inflammatory biomarkers and insulin resistance in obese men. Physiology and Behavior, 205:15-21.
27. Kralisch, S., Hoffmann, A., Klöting, N., Bachmann, A., Kratzsch, J., Blüher, M., Fasshauer, M. 2017. The novel adipokine/hepatokine fetuin B in severe human and murine diabetic kidney disease. Diabetes and Metabolism, 43(5):465-468.
28. Kralisch, S., Hoffmann, A., Lössner, U., Kratzsch, J., Blüher, M., Stumvoll, M., Ebert, T. 2017. Regulation of the novel adipokines/hepatokines fetuin A and fetuin B in gestational diabetes mellitus. Metabolism, 68:88-94.
29. Li, J., Li, Y., Chai, R., Yu, X., Yu, Z. 2022. PTX3 Protects Intestinal Mucosal Barrier Damage in Sepsis Through Toll-Like Receptor Signaling Pathway. Inflammation, 45(6):2339-2351.
30. Li, J., Yang, L., Qin, W., Zhang, G., Yuan, J., Wang, F. 2013. Adaptive induction of growth differentiation factor 15 attenuates endothelial cell apoptosis in response to high glucose stimulus. PLoS One, 8(6):e65549.
31. Li, L., Spranger, L., Stobäus, N., Beer, F., Decker, A.M., Wernicke, C., Mai, K. 2021. Fetuin-B, a potential link of liver-adipose tissue cross talk during diet-induced weight loss–weight maintenance. Nutrition and Diabetes, 11(1):1-7.
32. Li, Z., Lin, M., Liu, C., Wang, D., Shi, X., Chen, Z., Li, X. 2018. Fetuin-B links nonalcoholic fatty liver disease to type 2 diabetes via inducing insulin resistance: association and path analyses. Cytokine, 108:145-150.
33. Lockhart, S.M., Saudek, V., O’Rahilly, S. 2020. GDF15: a hormone conveying somatic distress to the brain. Endocrine Reviews, 41(4):bnaa007.
34. Miyaki, A., Choi, Y., Maeda, S.(2014. Pentraxin 3 production in the adipose tissue and the skeletal muscle in diabetic-obese mice. The American Journal of the Medical Sciences, 347(3):228-233.
35. Miyaki, A., Maeda, S., Choi, Y., Akazawa, N., Eto, M., Tanaka, K., Ajisaka, R. 2013. Association of plasma pentraxin 3 with arterial stiffness in overweight and obese individuals. American Journal of Hypertension, 26(10):1250-1255.
36. Moghadami, K., Shabani, M., Khalafi, M. 2020. The effect of aerobic training on serum levels of Growth differentiation factor-15 and insulin resistance in elderly women with metabolic syndrome. Daneshvar Medicine, 27(6);57-66.
37. Moghaddasi, Y., Ghazalian, F., Abediankenari, S., Ebrahim, K., Abednatanzi, H. 2020. Effect of aerobic and resistance training on gdf-15 levels in patients with type 1 diabetes. Journal of Mazandaran University of Medical Sciences, 30(186):123-132.
38. Mohd-Radzman, N.H., Ismail, W.I. W., Adam, Z., Jaapar, S.S., Adam, A. 2013. Potential roles of Stevia rebaudiana Bertoni in abrogating insulin resistance and diabetes: a review. Evidence-Based Complementary and Alternative Medicine, 2013:718049.
39. Moradi, M., Akbarnejhad, A., Choobineh, S., Shabkhiz, F. 2021. The effect of eight weeks of intense and continuous intermittent training on GDF15 and IL6 factor levels in overweight men. KAUMS Journal (FEYZ), 25(3):978-986.
40. Munk, P. S., Valborgland, T., Butt, N., & Larsen, A. I. 2011. Response of growth differentiation factor-15 to percutaneous coronary intervention and regular exercise training. Scandinavian Cardiovascular Journal, 45(1):27-32.
41. Norata, G.D., Marchesi, P., Pulakazhi Venu, V. K., Pasqualini, F., Anselmo, A., Moalli, F., Catapano, A.L. 2009. Deficiency of the long pentraxin PTX3 promotes vascular inflammation and atherosclerosis. Circulation, 120(8):699-708.
42. Oliveira, E.B.d., Gotschlich, C., Liu, T. 1979. Primary structure of human C-reactive protein. Journal of Biological Chemistry, 254(2):489-502.
43. Osorio-Conles, O., Guitart, M., Chacon, M.R., Maymo-Masip, E., Moreno-Navarrete, J.M., Montori-Grau, M., Gomez-Foix, A.M. 2011. Plasma PTX3 protein levels inversely correlate with insulin secretion and obesity, whereas visceral adipose tissue PTX3 gene expression is increased in obesity. American Journal of Physiology-Endocrinology and Metabolism, 301(6): E1254-E1261.
44. Patel, S., Alvarez-Guaita, A., Melvin, A., Rimmington, D., Dattilo, A., Miedzybrodzka, E.L., Meek, C.L. 2019. GDF15 provides an endocrine signal of nutritional stress in mice and humans. Cell Metabolism, 29(3):707-718.
45. Peter, A., Kovarova, M., Staiger, H., Machann, J., Schick, F., Königsrainer, A., Häring, H.U. 2018. The hepatokines fetuin-A and fetuin-B are upregulated in the state of hepatic steatosis and may differently impact on glucose homeostasis in humans. American Journal of Physiology-Endocrinology and Metabolism, 314(3):E266-E273.
46. Rangraz, E., Mirzaei, B., Nia, F. R. 2019. The effect of resistance training on serum levels of NT-proBNP, GDF-15, and markers of cardiac damage in the elderly males. International Journal of Applied Exercise Physiology, 8(1):138-148.
47. Saeidi, A., C Hackney, A., Tayebi, S. M., Ahmadian, M., Zouhal, H. 2019. Diabetes, insulin resistance, fetuin-B and exercise training. Annals of Applied Sport Science, 7(2):1-2.
48. Saeidi, A., Haghighi, M.M., Kolahdouzi, S., Daraei, A., Abderrahmane, A. B., Essop, M. F., Zouhal, H. 2021. The effects of physical activity on adipokines in individuals with overweight/obesity across the lifespan: A narrative review. Obesity Reviews, 22(1):e13090.
49. Saeidi, A., Nouri-Habashi, A., Razi, O., Ataeinosrat, A., Rahmani, H., Mollabashi, S.S., Al Kiyumi, M.H. 2023. Astaxanthin supplemented with high-intensity functional training decreases adipokines levels and cardiovascular risk factors in men with obesity. Nutrients, 15(2):286.
50. Saeidi, A., Shishvan, S. R., Soltani, M., Tarazi, F., Doyle-Baker, P.K., Shahrbanian, S., Moriarty, T.A. 2022. Differential effects of exercise programs on neuregulin 4, body composition and cardiometabolic risk factors in men with obesity. Frontiers in Physiology, 12:
51. Saeidi, A., Soltani, M., Daraei, A., Nohbaradar, H., Haghighi, M.M., Khosravi, N., VanDusseldorp, T. A. 2021. The effects of aerobic-resistance training and broccoli supplementation on plasma dectin-1 and insulin resistance in males with type 2 diabetes. Nutrients, 13(9):3144.
52. Salio, M., Chimenti, S., De Angelis, N., Molla, F., Maina, V., Nebuloni, M., Mantovani, A. 2008. Cardioprotective function of the long pentraxin PTX3 in acute myocardial infarction. Circulation, 117(8):1055-1064.
53. Samadi, A., Abbassi Daloii, A., Barari, A., Saeidi, A. 2020. The effect of twelve weeks of combined training with and without canagliflozin consumption on fetuin A and fetuin B in type 2 diabetic men. Journal of Basic Research in Medical Sciences, 7(4):20-30.
54. Sarookhani, M., & Arefian, E. 2014. Molecular mechanism of insulin resistance. Journal of Inflammatory Diseases, 18(5):57-64.
55. Shivanna, N., Naika, M., Khanum, F., Kaul, V. K. 2013. Antioxidant, anti-diabetic and renal protective properties of Stevia rebaudiana. Journal of Diabetes and its Complications, 27(2):103-113.
56. Slusher, A.L., Mischo, A.B., Acevedo, E.O. 2016. Pentraxin 3 is an anti-inflammatory protein associated with lipid-induced interleukin 10 in vitro. Cytokine, 86:36-40.
57. Slusher, A. L., Shibata, Y., Whitehurst, M., Maharaj, A., Quiles, J. M., Huang, C.J. 2017. Exercise reduced pentraxin 3 levels produced by endotoxin-stimulated human peripheral blood mononuclear cells in obese individuals. Experimental Biology and Medicine, 242(12):1279-1286.
58. Soleymani, K., Abbassi-Daloii, A., Ziaolhagh, S. J., Saeidi, A. 2021. The effect of aerobic training and stevia on the expression of miR-322 and cyclin D1 in heart tissue of type 1 diabetic rats. KAUMS Journal (FEYZ), 25(6):1286-1293.
59. Soleymani, K., Abbassi Daloii, A., Barari, A.R., Saeidi, A. 2020. Effect of Eight Weeks of Endurance Training and Stevia Supplemention on Atrial Natriuretic Peptide and β-Myosin Heavy Chain Expression Levels in Heart Tissue of Rats With Type 1 Diabetes. Journal of Inflammatory Diseases, 24(4):308-319.
60. Swindell, N., Mackintosh, K., McNarry, M., Stephens, J.W., Sluik, D., Fogelholm, M., Handjieva-Darlenska, T. 2018. Objectively measured physical activity and sedentary time are associated with cardiometabolic risk factors in adults with prediabetes: the PREVIEW study. Diabetes care, 41(3):562-569.
61. Takalooei, F., Shahrbanian, S., & C Hackney, A. 2022. Regulation of Metabolic Homeostasis by Reducing Selected Hepato-adipokines in Response to Three Types of Resistance Training in Men with Obesity. Journal of Basic Research in Medical Sciences, 9(1):50-61.
62. Teodoro, J. S., Gomes, A. P., Varela, A. T., Duarte, F. V., Rolo, A. P., & Palmeira, C. M. 2013. Uncovering the beginning of diabetes: the cellular redox status and oxidative stress as starting players in hyperglycemic damage. Molecular and cellular biochemistry, 376(1):103-110.
63. Triki, R., Zouhal, H., Chtourou, H., Salhi, I., Jebabli, N., Saeidi, A., Abderrahman, A.B. 2023. Timing of Resistance Training During Ramadan Fasting and Its Effects on Muscle Strength and Hypertrophy. International Journal of Sports Physiology and Performance, 1:1-11.
64. Tsai, V. W., Husaini, Y., Sainsbury, A., Brown, D. A., Breit, S.N. 2018. The MIC-1/GDF15-GFRAL pathway in energy homeostasis: implications for obesity, cachexia, and other associated diseases. Cell Metabolism, 28(3):353-368.
65. Verges, B. 2009. Lipid modification in type 2 diabetes: the role of LDL and HDL. Fundamental and Clinical Pharmacology, 23(6):681-685.
66. Wang, T., Guo, M., Song, X., Zhang, Z., Jiang, H., Wang, W., Zhang, N. 2014. Stevioside plays an anti-inflammatory role by regulating the NF-κB and MAPK pathways in S. aureus-infected mouse mammary glands. Inflammation, 37(5):1837-1846.
67. Witasp, A., Carrero, J.J., Michaëlsson, K., Ahlström, H., Kullberg, J., Adamsson, V., Lind, L. 2014. Inflammatory biomarker pentraxin 3 (PTX3) in relation to obesity, body fat depots and weight loss. Obesity, 22(5):1373-1379.
68. Xiong, Y., Walker, K., Min, X., Hale, C., Tran, T., Komorowski, R., Kemp, D. 2017. Long-acting MIC-1/GDF15 molecules to treat obesity: Evidence from mice to monkeys. Science translational medicine, 9(412):eaan8732.
69. Zaidan, U. H., Zen, N. I. M., Amran, N. A., Shamsi, S., Abd Gani, S.S. 2019. Biochemical evaluation of phenolic compounds and steviol glycoside from Stevia rebaudiana extracts associated with in vitro antidiabetic potential. Biocatalysis and agricultural biotechnology, 18:10
70. Zangeneh, M., Najafi, F., Tahvilian, R., Haghnazari, L., Zangeneh, A., Abiari, M., Moradi, R. 2016. Study on the in vitro antibacterial properties of alcoholic extract of Stevia rebaudiana in west of Iran. International Journal of Scientific Engineering and Research, 7(11):1352-1359.
71. Zempo-Miyaki, A., Fujie, S., Sato, K., Hasegawa, N., Sanada, K., Maeda, S., Iemitsu, M. 2016. Elevated pentraxin 3 level at the early stage of exercise training is associated with reduction of arterial stiffness in middle-aged and older adults. Journal of human hypertension, 30(9):521-526.
72. Zempo-Miyaki, A., Kumagai, H., Yoshikawa, T., Myoenzono, K., So, R., Otsuki, T., Maeda, S. 2019. Pentraxin 3 increases in adult overweight and obese men after weight loss by dietary modification with exercise training. Applied Physiology, Nutrition, and Metabolism, 44(2):111-117.
73. Zouhal, H., Ben Abderrahman, A., Khodamoradi, A., Saeidi, A., Jayavel, A., Hackney, A.C., Jabbour, G. 2020. Effects of physical training on anthropometrics, physical and physiological capacities in individuals with obesity: A systematic review. Obesity Reviews, 21(9):e13039.
74. Zouhal, H., Rhibi, F., Salhi, A., Jayavel, A., Hackney, A.C., Saeidi, A., Abderrahman, A.B. 2023. The effects of exercise training on plasma volume variations: A systematic review. International Journal of Sports Medicine. doi: 10.1055/a-1667-6624.
75. Zouhal, H., Zare-Kookandeh, N., Haghighi, M.M., Daraei, A., de Sousa, M., Soltani, M., Laher, I. 2021. Physical activity and adipokine levels in individuals with type 2 diabetes: A literature review and practical applications. Reviews in Endocrine and Metabolic Disorders, 22(4): 987-1011