Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique

Journal of Chemical Health Risks

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 خرداد 1402   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.22034/jchr.2023.1981240.1694

نویسندگان

Nosrat Musavi¹؛ Mostafa Ebadi^{* 1}؛ Mehdi Khorshidi²؛ Nahid Masoudian¹؛ Hossein Hokmabadi³

¹Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran

²Assistant Professor, Biology School, Damghan University, Damghan, Iran

³Associate Professor, Agricultural Education and Extension Institute, AREEO, Tehran, Iran

چکیده

One of the most important crop plants is potato. Plant tissue culture is a very important technology in plant cultivation. In this investigation, the biochemical characterizes are investigated of potatoes cultivated by the tissue culture method. The lateral and apical buds were separated from the stem. They were sterilized and cultured in Murashige and Skoog culture medium. The concentrations of salt (0, 25, 50, 75 and 100 mM) have been used for salinity. After the growth of buds, the leaves were sampled and the chlorophylls, proline, sugars, protein, and antioxidant enzymes activity such as catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase were evaluated. The results indicate that salt decreased protein and chlorophylls content. Sugars content and fresh weight have also been reduced with salinity, but the content of proline, malondialdehyde, and hydrogen peroxide were increased under salinity stress. Phenolic compounds, anthocyanin content, and antioxidant enzymes activity increased up to 50 mM salinity, but they decreased above this concentration. According to these results, it can be suggested that Agria cultivar potatoes are not recommended in salinity higher than 50 mM.

کلیدواژه‌ها

Physiological properties؛ Potato؛ Salinity stress؛ Tissue culture

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