Molecular docking, anti-inflammatory, antimicrobial and antioxidant evaluation of Pterospermum rubiginosum B. Heyne

Trends in Phytochemical Research

مقاله 3، دوره 7، شماره 2، شهریور 2023، صفحه 95-109   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/tpr.2023.1977515.1316

نویسندگان

Rajamohanan Jalaja Anish¹؛ Fathima Rumaisa¹؛ Thankamani Ravikumar Aswathy²؛ Velappan Nair Saralakumari Kalpana³؛ Arun A Rauf^{* 1}

¹Department of Biochemistry, University of Kerala, Trivandrum, India

²Department of Computational Biology and Bioinformatics, University of Kerala, Trivandrum, India

³Department of Biochemistry, University of Kerala, Trivandrum, India Kerala, India.

چکیده

Pterospermum rubiginosum B.Heyne ex G.Don (PR) is a traditional medicinal plant used by the tribal people of the Western Ghats to treat bone fractures, inflammation, and sprain. Being an under-explored medicinal plant, the mechanism behind the anti-inflammatory activity of PR is unknown to the scientific community. FTIR analysis was done to recognize the functional groups in PR bark extract, and LCMS elucidated the phytochemical characterization. Molecular docking studies showed an excellent ligand and protein (inflammatory mediators) binding and their interactive mechanism. Total phenolic and total flavonoid contents were found to be 46.7 and 37.4 g/100 g for PRME. PRME showed hydrogen peroxide and DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging activity with IC50 values of 48.33 and 42.70 µg/mL, respectively. Gene expression study of COX-1 and COX-2 enzymes confirmed the anti-inflammatory activity of PRME. The present study justifies the potential use of PR in the traditional medicine as an anti-inflammatory agent.  

کلیدواژه‌ها

Anti-inflammatory؛ Bark extract؛ Malvaceae؛ Molecular docking؛ Pterospermum rubiginosum B. Heyne؛ RAW 264.7cells

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