African violets Organogenesis Responding to Hormonal Interaction

Iranian Journal of Plant Physiology

دوره 13، شماره 2، تیر 2023، صفحه 4487-4494   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2022.1922952.1301

نویسندگان

Maria Missaghi¹؛ Fataneh Yari^{* 2}؛ Amir Mousavi³؛ Younes Mostofi⁴؛ Hamideh Ofoghi⁵

¹Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran

²Department of Agriculture, Iranian Research Organization for Science and Technology, P.O. Box: 33535111, Tehran, Iran.

³Department of Plant Molecular Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran.

⁴Department of Horticultural Sciences, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources. University of Tehran, P.O. Box4111, Karaj, Iran.

⁵Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran

چکیده

In this study, the possibility of organogenesis of leaves and petioles of two African violet cultivars were evaluated using MS media containing various concentration of plant growth regulators (IAA (1,2 mg/l), BA (0.05, 0.1, 0.2, 0.5 mg/l), NAA (1, 2 mg/l), and TDZ (0.01 mg/l)). All experiments were arranged completely randomized design then data analyzed by one-way ANOVA, and means were separated using Duncan’s new multiple range test (𝑃 ≤ 0.01). Direct and indirect organogenesis from the whole leaf with petiole bases were observed in both cultivars. However, the separated leaf blade and petiole were unable to produce any organs. The organogenesis potential of cultivars was significantly different, although the medium containing BA (0.1 mg/l) + IAA (1 mg/l) showed the highest indirect organogenesis (90 %< in both cultivars). In contrast, the media containing BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 2 mg/l and BA 0.05 mg/l + TDZ 0.01 mg/l + IAA 1 mg /l had more success on the induction of direct organogenesis. Therefore, the level of direct and indirect organogenesis appears to be dependent on the interaction between the PGRs and the explant type having a direct correlation with the genotype.

کلیدواژه‌ها

Saintpaulia ionantha؛ Tissue culture؛ PGRs؛ Regeneration؛ Genotype

مراجع

Bakhshaie, M., Khosravi, S., Azadi, P., Bagheri, H., and van Tuyl, J. M, 2016. 'Biotechnological advances in Lilium'. Plant Cell Reports, 35(9), 1799–1826.

Bhatla, S. C, 2018. 'Plant Growth Regulators: An Overview'. Plant Physiology, Development and Metabolism, 559–568.

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I. and Ninković, S, 2021. 'Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi'. Scientific Reports, 11(1).

Dinani, E. T., Shukla, M. R., Turi, C. E., Sullivan, J. A., and Saxena, P. K, 2018. 'Thidiazuron: Modulator of morphogenesis in vitro'. Thidiazuron: From Urea Derivative to Plant Growth Regulator, 1–36.

Gehl, C., Li, G., and Serek, M, 2020. 'An efficient protocol for Agrobacterium-mediated transformation and regeneration of Campanula medium (Canterbury bells) based on leaf disc explants'. Plant Cell, Tissue and Organ Culture, 140(3), 635–645.

Keutgen, N., Figas, A. T., Tomaszewska-Sowa, M., Raunest, K.,  Keutgen, A. J, 2016. 'Chlorophyll Fluorescence as a Tool to Assess the Regeneration Potential of African Violet Leaf Explants'. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(1), 11–16.

Lee, J. H., and Pijut, P. M, 2017. 'Adventitious shoot regeneration from in vitro leaf explants of Fraxinus nigra'. Plant Cell, Tissue and Organ Culture, 130(2), 335–343.

Mekala, G. K., Juturu, V. N., Mallikarjuna, G., Kirti, P. B. and Yadav, S. K, 2016. 'Optimization of Agrobacterium-mediated genetic transformation of shoot tip explants of green gram (Vigna radiata (L.) Wilczek)'. Plant Cell, Tissue and Organ Culture, 127(3), 651–663.

Mladenović, E., Zorić, M., Čukanović, J., Hiel, K., Pavlović, L., and Sentić, I, 2016.  'Effect of Plant Growth Regulators on the Propagation of African Violet (Saintpaulia ionantha H. Wendl.) from Leaf Cuttings’. Contemporary Agriculture, 65(3–4), 63–67.

Mose, W., Indrianto, A., Purwantoro, A., and Semiarti, E, 2017. 'The Influence of Thidiazuron on Direct Somatic Embryo Formation from Various Types of Explant in Phalaenopsis amabilis (L.) Blume Orchid'. HAYATI Journal of Biosciences, 24(4), 201–205.

Neumann, K.-H., Kumar, A., and  Imani, J, 2020. Plant Propagation: Meristem Cultures, Somatic Embryogenesis Micropropagation, and Transformation of Somatic Embryos in Bioreactors. In Plant Cell and Tissue Culture – A Tool in Biotechnology,107–183.

Nguyen, N. H., and Van Le, B, 2020. 'A simple, economical, and high efficient protocol to produce in vitro miniature rose'. In Vitro Cellular and Developmental Biology - Plant, 56(3), 362–365.

Ramakrishna, A., Gill, S. S., Sharma, K. K., Tuteja, N., and Ravishankar, G. A, 2016. 'Indoleamines (Serotonin and Melatonin) and Calcium-Mediated Signaling in Plants'. Serotonin and Melatonin, 85–96.

Sarai, N., Bodhipadma, K., Noichinda, S., Luangsriumporn, P., and Leung, D. W. M, 2017. 'Microshoot culture of Persian violet: Plant regeneration and in vitro flowering'. Annals of Agricultural Sciences, 62(1).

Silva, J. A. T. Da, Dewir, Y. H., Wicaksono, A., Sahijram, L., Kim, H., Zeng, S., Chandler, S. F., and Hosokawa, M, 2017. 'African violet (Saintpaulia ionantha H. Wendl.): Classical breeding and progress in the application of biotechnological techniques'. Folia Horticulturae 29 (2), 99–111.

Teixeira da Silva, J. A., Zeng, S., Wicaksono, A., Kher, M. M., Kim, H., Hosokawa, M.,  and Dewir, Y. H, 2017. 'In vitro propagation of African violet: A review'. South African Journal of Botany, 112, 501-507.

Teixeira, J. A., Silva, D., Dewir, Y. H., Wicaksono, A., Kher, M. M., Kim, H., Hosokawa, M.,  Zeng, S, 2016. 'Morphogenesis and developmental biology of African violet (Saintpaulia ionantha H. Wendl.)'. Journal of Plant Development, 23, 15–25.

Vinoth, A., and Ravindhran, R, 2018. 'In vitro morphogenesis of woody plants using thidiazuron'. Thidiazuron: From Urea Derivative to Plant Growth Regulator, 211–229.

Zeng, S., Huang, W., Wu, K., Zhang, J., Teixeira Da Silva, J. A., and Duan, J, 2016. 'In vitro propagation of Paphiopedilum orchids'. Critical Reviews in Biotechnology, 36(3), 521–534.