Process optimization of corn starch nanoparticles containing linalyl acetate: characterization and antibacterial properties

International Journal of Industrial Chemistry

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 31 مرداد 1402

نوع مقاله: research article

نویسندگان

Milad Gholami¹؛ Morteza Hosseini¹؛ Mohammad Hassan Shahavi^{* 2}؛ Mohsen Jahanshahi¹

¹Department of Chemical Engineering, Babol Noshirvani University of Technology, Babol, Iran

²Department of Nanotechnology, Faculty of Engineering Modern Technologies, Amol University of Special Modern Technologies (AUSMT), Amol, Iran

چکیده

In this research, the Taguchi approach optimized the preparation process of corn starch nanoparticles containing linalyl acetate (extracted from lavender). Sodium hydroxide and urea (solvent), ethanol (anti-solvent), and Tween 80 (surfactant) were employed for the synthesis in the nanoprecipitation method. Dimensions of the synthesized nanoparticles were measured by the dynamic light scattering method. The surface morphology of the nanoparticles was determined by atomic force microscopy and scanning electron microscopy. The results showed that the concentration of starch (2%w/w) and linalyl acetate (1.5%w/w), temperature (55°C), and the starch to the antisolvent ratio (1:15) had a significant effect on the size of the corn starch nanoparticle with the linalyl acetate. The predicted values for mean size، PDI and zeta potential were 70 nm، 0.44 and -19.4 mV respectively. Accordingly, the experimental values for mean size، PDI, and zeta potential were 71 nm، 0.47, and -22.7 mV, respectively. The size of nanoparticles was 71 nm with a spherical shape under the optimum condition. The optimal formula of nanoparticles' antibacterial properties against a gram-negative bacterial strain (Escherichia coli) and a gram-positive bacterial strain (Staphylococcus aureus) was evaluated. The maximum diameter of the inhibition zone determined by disk diffusion and well diffusion methods was 31 and 33 mm for Staphylococcus aureus and 15 and 20 mm for Escherichia coli. The minimum inhibitory concentration and minimum bactericidal concentration were observed at 33 mg/ml for Escherichia coli and 22 mg/ml for Staphylococcus aureus which has a relatively good ability to inhibit these bacteria's growth.

کلیدواژه‌ها

Antibacterial؛ Corn Starch Nanoparticles؛ Linalyl Acetate؛ Nanoprecipitation؛ Taguchi