Quantitative Analysis of β-Blockers in Water Samples by HPLC Using Directly Suspended Droplet Microextraction

International Journal of Industrial Chemistry

دوره 12، شماره 2، آبان 2021

نوع مقاله: research article

شناسه دیجیتال (DOI): 10.47176/IJIC.12.2.1

نویسنده

Mohammad Reza Abedi^*

Department of Applied Chemistry, Quchan Branch, Islamic Azad University, Quchan, Iran

چکیده

The aim of this study is using Directly Suspended Droplet Microextraction (DSDME) as the sensitive drug assays in environmental waters. This work explains the success of this liquid-phase microextraction technique used as sample pre-concentration technique. This attractive method, which is selective and enable substantial pre-concentration, has been used for determination of three β-blockers; Atenolol, Metoprolol and Propranolol as the model compounds. The effective parameters such as organic solvent, pH of donor and acceptor phases, phase volumes, extraction and back-extraction time, stirring rate and addition of salt are discussed. The extracted β-blockers were analyzed at room temperature by High Performance Liquid Chromatography (HPLC), equipped an Agilent Eclipse XDB-C18 column with particle size of 5μm (250mm×4.6 mm i.d.) and programmed wavelength fluorescence detector. The mobile phase was 0.01molL-1 NaH2PO4 (adjusted to pH 3.0 with phosphoric acid)-methanol-acetonitril (45:45:10, v:v:v). The flow rate was 1.0 mLmin-1. The separated β-blockers were detected using fluorometric detection. The results showed that practical pre-concentration factors varied from 34.8 to 171.6.

کلیدواژه‌ها

β-blockers؛ Directly Suspended Droplet Microextraction (DSDME)؛ Fluorescence Detection؛ HPLC