The Effects of Eight Weeks of Submaximal Running and High-fat Diet on IL-6 Gene Expression in the Muscle Tissue of Male Rats

New Researches of Management in Sport and Health

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 مهر 1402   

نوع مقاله: Original Article

نویسندگان

Shima Ebadi؛ Ardeshir Zafari^*

Department of Sports Sciences, Faculty of Humanities and Art, Zanjan Branch, Islamic Azad University, Zanjan, Iran

چکیده

It seems that the release of IL-6 from the contracting muscles and accumulation in the blood circulation has a close relationship with the duration and intensity of physical activity. The aime of this experimental study was to determine the effects of eight weeks of submaximal running and high-fat diet on IL-6 gene expression levels in the muscle tissue of an animal model. At the end of the two-week period of keeping under controlled conditions, 40 two-month-old male rats were randomly divided into four equal groups of control, high-fat diet, submaximal running, and a combination of high-fat diet and submaximal running. The training groups participated in the aerobic running protocol on the treadmill for five days a week for eight weeks. The polymerase chain reaction method was used to determine the levels of IL-6 gene expression in muscle tissue. One-way analysis of variance was used to determine between-group differences. The effects of high-fat diet and running with submaximal intensity on IL-6 gene expression in muscle tissue are significant. The consumption of high-fat food causes adverse changes in the expression of IL-6 gene, but eight weeks of running with sub-maximal intensity moderates the adverse effects of high-fat diet and creates favorable changes in the expression of IL-6 gene and body weight.

کلیدواژه‌ها

Body weight؛ High-fat diet؛ Inter Leukin-6؛ Aerobic training

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