Alpha Lipoic Acid Upregulates Mitochondrial DNA Copy Number of Mouse Ovarian Follicles during In Vitro Culture

Journal of Chemical Health Risks

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 05 آذر 1402   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.22034/jchr.2023.1984395.1730

نویسندگان

Sara Seifi¹؛ Saeed Zavareh^{* 1، 2}؛ Meysam Nasiri^{1، 2}

¹School of Biology, Damghan University, Damghan, Iran

²Institute of Biological Sciences, Damghan University, Damghan, Iran

چکیده

The objective of this study was to investigate the influence of alpha lipoic acid (ALA) on the expression of the Tfam gene and the copy number of mtDNA in preantral follicles (PFs) of mice during in vitro culture. PFs were mechanically isolated from 14-day-old National Medical Research Institute (NMRI) mice and subjected to a 12-day culture in the presence of 100µm ALA, followed by hCG-induced ovulation. The developmental parameters of PFs and oocyte maturation were evaluated, and the expression of the Transcription Factor A, Mitochondrial (Tfam) gene, along with mtDNA copy number in granulosa cells and oocytes, were measured using real-time PCR. The results showed that ALA-treated groups exhibited significantly higher diameter of PFs and survival rate, antrum formation, and MII oocytes rate in comparison to the control groups (P<0.05). Furthermore, Tfam gene expression significantly increased in granulosa cells and oocytes of the ALA groups in comparison with the control group, and the mtDNA copy number in granulosa cells and oocytes of the ALA-treated group was significantly higher than that of the control groups (P<0.05). Supplementing the culture medium with ALA demonstrated an improvement in PFs' developmental competence, likely mediated by the upregulation of Tfam gene expression and an increase in mtDNA copy number in oocytes and granulosa cells.

کلیدواژه‌ها

Alpha lipoic acid؛ Ovarian follicle؛ Tfam؛ mtDNA

مراجع

1. Herta A.C., Lolicato F., Smitz J.E.J., 2018. In vitro follicle culture in the context of IVF. Reproduction. 156(1), F59-f73.
2. Agarwal A., Maldonado Rosas I., Anagnostopoulou C., Cannarella R., Boitrelle F., Munoz L.V., Finelli R., Durairajanayagam D., Henkel R., Saleh R., 2022. Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment. Antioxidants. 11(3), 477.
3. Sasaki H., Hamatani T., Kamijo S., Iwai M., Kobanawa M., Ogawa S., Miyado K., Tanaka M., 2019. Impact of Oxidative Stress on Age-Associated Decline in Oocyte Developmental Competence. Front Endocrinol (Lausanne). 10, 811.
4. Valko M., Rhodes C.J., Moncol J., Izakovic M., Mazur M., 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 160(1), 1-40.
5. Petca A., Bot M., Maru N., Calo I.G., Borislavschi A., Dumitrascu M.C., Petca R.C., Sandru F., Zvanca M.E., 2021. Benefits of α‑lipoic acid in high‑risk pregnancies (Review). Exp Ther Med. 22(5), 1232.
6. Gabriel M.S., Chan S.W., Alhathal N., Chen J.Z., Zini A., 2012. Influence of microsurgical varicocelectomy on human sperm mitochondrial DNA copy number: a pilot study. J Assist Reprod Genet. 29(8), 759-764.
7. St John J., 2014. The control of mtDNA replication during differentiation and development. Biochim Biophys Acta. 1840(4), 1345-1354.
8. Benkhalifa M., Ferreira Y.J., Chahine H., Louanjli N., Miron P., Merviel P., Copin H., 2014. Mitochondria: participation to infertility as source of energy and cause of senescence. Int J Biochem Cell Biol. 55, 60-64.
9. Desquiret-Dumas V., Clément A., Seegers V., Boucret L., Ferré-L'Hotellier V., Bouet P. E., Descamps P., Procaccio V., Reynier P., May-Panloup P., 2017. The mitochondrial DNA content of cumulus granulosa cells is linked to embryo quality. Hum Reprod. 32(3), 607-614.
10. Bouda E., Stapon A., Garcia-Diaz M., 2019. Mechanisms of mammalian mitochondrial transcription. Protein Sci. 28(9), 1594-1605.
11. Hatami S., Zavareh S., Salehnia M., Lashkarbolouki T., Ghorbanian M.T., Karimi I., 2013. Total Oxidative status of Mouse Vitrified Pre-Antral Follicles with Pre-Treatment of Alpha Lipoic Acid. Iranian Biomedical Journal. 18(3), 181-183.
12. Hatami S., Zavareh S., Salehnia M., Lashkarbolouki T., Ghorbanian M.T., Karimi I., 2014. The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries. Iranian Journal of Reproductive Medicine. 12(1), 57.
13. Talebi A., Zavareh S., Kashani M.H., Lashgarbluki T., Karimi I., 2012. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. Journal of Assisted Reproduction and Genetics. 29(2), 175-183.
14. Zavareh S., Karimi I., Salehnia M., Rahnama A., 2016. Effect of in vitro maturation technique and alpha lipoic acid supplementation on oocyte maturation rate: focus on oxidative status of oocytes. International Journal of Fertility & Sterility. 9(4), 442.
15. Zavareh S., Talebi A., Hasanzadeh H., 2015. Amending in vitro culture condition to overcome oxidative stress in assisted reproduction techniques (ART). Archives of Advances in Biosciences. 6(2), 135-148.
16. Ghorbanmehr N., Salehnia M., Amooshahi M., 2018. The Effects of Sodium Selenite on Mitochondrial DNA Copy Number and Reactive Oxygen Species Levels of In Vitro Matured Mouse Oocytes. Cell J. 20(3), 396-402.
17. Kasapoğlu I., Seli E., 2020. Mitochondrial Dysfunction and Ovarian Aging. Endocrinology. 161(2), 10.1210/endocr/bqaa001
18. Zoheir K., Harisa G., Allam A., Liguo Y., Xiang L., Liang A., Abd-Rabou A., Harrath H., 2016. Effect of alpha lipoic acid on in vitro development of bovine secondary preantral follicles. Theriogenology. 88, 10.1016/j.theriogenology.2016.09.013.
19. Deng X., Lin N., Fu J., Xu L., Luo H., Jin Y., Liu Y., Sun L., Su J., 2020. The Nrf2/PGC1α Pathway Regulates Antioxidant and Proteasomal Activity to Alter Cisplatin Sensitivity in Ovarian Cancer. Oxid Med Cell Longev. 4830418.
20. Zhang H., Wu B., Liu H., Qiu M., Liu J., Zhang Y., Quan F., 2013. Improving development of cloned goat embryos by supplementing α-lipoic acid to oocyte in vitro maturation medium. Theriogenology. 80(3), 228-233.
21. Fernández-Galilea M., Pérez-Matute P., Prieto-Hontoria P.L., Houssier M., Burrell M.A., Langin D., Martínez J.A., Moreno-Aliaga M.J., 2015. α-Lipoic acid treatment increases mitochondrial biogenesis and promotes beige adipose features in subcutaneous adipocytes from overweight/obese subjects. Biochim Biophys Acta. 1851(3), 273-281.
22. Kim C., Moon J., Jeung Y., Kim S., Chae H., Kang B., 2017. Lipoic acid supplementation increases the expression of pgc-1alpha gene in granulosa cells and improves IVF results in aging women undergoing IVF. Fertility and Sterility. 108(3, Supplement), e228.
23. Bogenhagen D.F., 2012. Mitochondrial DNA nucleoid structure. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1819(9), 914-920.