The Effect of Maltose on Water Buffalo (Bubalus bubalis) Post Mortem Epididymal Sperm Kinetics

Journal of Basic and Clinical Veterinary Medicine

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 02 دی 1402

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/jbcvm.2023.1993667.1045

نویسندگان

Mojtaba Dolatpanah؛ Keivan Abdy^*

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

چکیده

Maltose disaccharide during cell glycolysis is easily converted into two glucose molecules and it is provided as a source of energy for sperm motility. To evaluate the effect of maltose on water buffalo epididymal sperm kinetics, after the usual industrial slaughter, 10 pairs of buffalo bull testicles, were transported to the laboratory of the veterinary faculty, in a styrofoam box with a temperature of 5o to 8o Celsius. In the lab, after fixation of the tail of the epididymis with two fingers, multiple incisions were made in none capillary zones. After extraction of the milky-colored liquid containing concentrated sperm, this liquid was transferred to Eppendorf containing cell culture medium with 10 percent bovine serum albumin (BSA). Five levels of maltose sugar (1, 3, 5, 10, 15 mM) were added to the Eppendorf containing 1 ml of culture medium and 30-40 million sperm with 10% of BSA and were incubated for 24 hours at 37o Celsius. Sperm kinetics was assessed at 1,6, 12, and 24 hrs by computer-assisted sperm analysis (CASA). Statistical analysis revealed that CASA data such as rapid progressive motility (Class A, %) progressive motility (Class B, %), viability (Class A+B+C, %), straight-line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), average path velocity (VAP, µm/s) and amplitude of lateral head (ALH, µm) was higher at 24 hrs in maltose group than control (p<0.05). In conclusion, this study suggests maltose shows desired effect for buffalo epididymal sperm, and maltose maybe act as an energy substrate for sperm motility.

کلیدواژه‌ها

Sperm"؛ "Maltose"؛ "CASA"؛ "Buffalo