Investigating the impact of Lactobacillus lactis TISTR 1464 on growth performance, hematological parameters, digestive enzyme activity, immune response, and post-probiotic status in Nile tilapia (Oreochromis niloticus)

International Aquatic Research

مقاله 4، دوره 16، شماره 1، خرداد 2024، صفحه 39-54   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2024.1995330.1503

نویسندگان

Mahattanee Phinyo^{* 1}؛ Thidarat Chitma²؛ Phattharamat Thinjan²؛ Thanita Boonsrangsom¹؛ Jirawat Mala³

¹Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok 65000, Thailand

²Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand

³Program in Public Health, Faculty of Science and Technology, Suratthani Rajabhat University, Suratthani 84100, Thailand

چکیده

This study examined the effects of different levels of supplementation with Lactobacillus lactis TISTR 1464 on the growth performance, hematological parameters, digestive enzymes, and immune response in of Nile tilapia (Oreochromis niloticus) one week post-cessation. Tilapia were fed diets supplemented with L. lactis at concentrations of 0 (control), 104, 106, and 108 CFU/g of diet in three replicates. The initial average weight of tilapia was 8.08±0.01 g. Throughout the 10-week supplementation period, the growth of tilapia was not significantly affected by different concentrations of L. lactis (P >0.05). However, tilapia on a diet supplemented with 108 CFU/g of L. lactis had higher whole-body crude lipid levels than the other groups (P <0.05). Additionally, they showed elevated lipase activity (P <0.05). Fish fed diets supplemented with 106 and 108 CFU/g of L. lactis demonstrated significantly higher levels of hematocrit, hemoglobin, and white blood cells (WBC) compared to those fed the control diet. No significant differences were found in cholesterol, albumin, globulin, and liver function biomarkers among the dietary groups. Fish on a diet supplemented with 108 CFU/g of L. lactis also exhibited increased levels of catalase (CAT), superoxide dismutase, and lysozyme (P <0.05), with no difference in myeloperoxidase. After one week of cessation, CAT levels in fish fed L. lactis supplemented diet remained considerably higher than those in the control group (P <0.05), despite no noticeable differences in red blood cells (RBC) and WBC. In conclusion, adding 108 CFU/g of L. lactis to the Nile tilapia diet boosted lipase activity and stimulated immune responses. However, one week post-cessation, the immune response diminished, suggesting that continuous dietary supplementation with L. lactis is necessary for enhancing immune response in Nile tilapia.

کلیدواژه‌ها

Lactobacillus lactis؛ Probiotic؛ Immunostimulant؛ Cessation probiotic