Synergistic Antimicrobial Potential of Pistacia atlantica Gum Essential Oil and Camellia sinensis Methanolic Extract Against Pseudomonas aeruginosa

International Journal of Molecular and Clinical Microbiology

دوره 13، شماره 2، آذر 2023، صفحه 1952-1958   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/ijmcm.2023.710008

نویسندگان

shabnam Bahrami¹؛ Abbas Ali Imani Fooladi²؛ Mansour Bayat³؛ Farzaneh Chehelcheraghi⁴؛ Setareh Soroush^{* 5}

¹Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Applied Microbiology Research Center, System Biology and Poisoning Institute Baqiyatallah University of Medical Sciences. Tehran Iran

³Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

⁴Razi Herbal Medicines Research Center, Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

⁵Razi Herbal Medicines Research Center, Department of Microbiology, Faculty of Medicine Lorestan University of Medical Sciences, Khorramabad, Iran

چکیده

Pseudomonas aeruginosa, a versatile pathogen causing a wide range of infections, faces escalating resistance to conventional antibiotics, necessitating exploration of alternative substances. Plant extracts and essential oils, known for potent antibacterial compounds, emerge as promising candidates to combat P. aeruginosa effectively. Essential oil from Pistacia atlantica gum was extracted using a Clevenger apparatus, and Camellia sinensis (green tea) extract was obtained through maceration. Gas chromatography/mass spectrometry (GC-MS) and spectrophotometry identified compounds in the essential oil and extract. Antibacterial properties were assessed using the microdilution technique, and synergistic effects of combined substances were evaluated through the modified checkerboard method, utilizing the Fractional Inhibitory Concentration (FIC) index. P. aeruginosa (ATCC27853) was the target bacteria. Alpha-pinene and polyphenols were identified as predominant compounds in P. atlantica gum essential oil and C. sinensis extract, respectively. Both compounds exhibited comparable Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, at 625 and 1250 μg/ml, respectively. The FIC index calculation for the combination of essential oil and extract demonstrated enhanced antibacterial activity against P. aeruginosa. Results affirm the inhibitory and bactericidal properties of P. atlantica gum essential oil and C. sinensis extract against P. aeruginosa. Concurrent use exhibited synergistic antibacterial effects. This study suggests that these natural compounds, especially when combined, could serve as potent agents in the battle against P. aeruginosa infections, offering a potential avenue for the development of alternative antimicrobial strategies.

کلیدواژه‌ها

Pseudomonas aeruginosa؛ pistacia atlantica؛ Camellia sinensis؛ Minimum inhibitory concentration؛ Minimum bacteriocidal concentration؛ Fractional Inhibitory Concentration