Effect of extracted bacteriocin L. plantarum ATCC8014 on the expression of FUM1 secreted by Fusarium verticillioides

International Journal of Molecular and Clinical Microbiology

دوره 14، شماره 1، مهر 2024، صفحه 2027-2037   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/ijmcm.2024.710056

نویسندگان

Mahtab Afrashi¹؛ Mansour Bayat^{* 2}

¹Department of Microbiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده

The Fusarium fungus is a pest that harms food plants and affects agricultural production. One way to control this fungus is by using biological methods, such as employing bacteria from the lactic acid family. In this study, we researched the impact of bacteriocin produced by Lactobacillus plantarum (L. plantarum) on the growth of Fusarium verticillioides (F. verticillioides) and the expression of FUM1, a protein secreted by this fungus. We acquired ten confirmed strains of F. verticillioides (f1-f10) and one strain of L. plantarum ATCC8014 from the Atiyeh Salamat Alborz company laboratory. The impact of bacteriocin extracted from L. plantarum on inhibiting the growth of the fungus and on FUM1 gene expression in F. verticillioides was examined using specific primers and the RT-PCR method. The research data revealed that the fungus growth was halted in the concentration range of 0.014 to 0.113 μg/ml (with an average μg/ml MIC of 0.42). Further, the lethal concentration of the bacteriocin (MFC) ranged between 0.028 and 0.225 μg/ml. After treatment with this bacteriocin, electrophoresis of the RT-PCR product showed that two sensitive strains, f4 and f8 (20% of the total 10 isolates), exhibited a complete lack of gene expression. L. Plantarum is highly effective in preventing the growth of F.Verticillioides. It can also prevent Fumonisin toxin production completely in two strains, making it a promising candidate to inhibit the fungus.

کلیدواژه‌ها

FUM1، RT-PCR ، MIC، MFC؛ Fusarium verticillioides