Bacterial microbiota of African catfish Clarias gariepinus during the hatchery and nursery phases of culture in the Philippines

International Aquatic Research

مقاله 4، دوره 17، شماره 2، شهریور 2025، صفحه 149-164   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2025.2009291.1840

نویسندگان

Rolando V Pakingking JR^{* 1}؛ Faye Danielle Solis²؛ Peter Palma³؛ Leobert dela Peňa²

¹College of Medical Laboratory Science, Central Philippine University, Jaro, Iloilo City 5000, Iloilo, Philippines

²Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC AQD), Tigbauan 5021, Iloilo, Philippines

³University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom

چکیده

The bacterial microbiota of African catfish (Clarias gariepinus) during hatchery and nursery phases were examined across eight production cycles in two commercial farms in the Philippines. Bacterial identification was performed using conventional methods and the API 20E test, with further confirmation of selected isolates via 16S rRNA gene sequencing. Heterotrophic plate count (HPC), presumptive Aeromonas count (PAC), and presumptive Vibrio count (PVC) varied in hatchery rearing water (10³–10⁵ CFU/mL for HPC; 10²–10⁴ CFU/mL for PAC and PVC) and fry samples (10⁴–10⁷ CFU/g for HPC; undetectable–10⁶ CFU/g for PAC; 10²–10⁴ CFU/g for PVC). In the nursery phase, similar fluctuations were observed in rearing water, fingerlings, and sediment. Microbial diversity analysis revealed 21 species (15 genera) in hatchery rearing water and 14 species (8 genera) in fry, with Aeromonas hydrophila, A. sobria, and Vibrio cholerae as predominant species (>15%). In the nursery phase, 29 species (16 genera), 28 species (15 genera), and 21 species (14 genera) were identified from rearing water, sediment, and fingerlings, respectively, with A. hydrophila, A. sobria, and Bacillus subtilis dominating (>14%). This study highlights the dynamic composition of bacterial communities in catfish culture systems, with rearing environments shaping the microbiota of juvenile fish. The presence of opportunistic and zoonotic pathogens underscores the risk of disease outbreaks, particularly under suboptimal conditions. These findings emphasize the need for stringent biosecurity measures and improved hatchery and nursery management practices to enhance fish health, prevent economic losses, and ensure the sustainability of aquaculture production. Educating hatchery and nursery workers on safe aquaculture practices and hygiene protocols is crucial to minimizing health risks to both fish and humans while ensuring efficient and responsible catfish production.

کلیدواژه‌ها

Aeromonas spp. . Bacterial load . Zoonotic potential . African catfish . Bacterial flora