Pilot-scale cultivation of red microalga Porphyridium purpureum culture in a greenhouse for assessment of production potential under mid-latitude climate conditions

International Aquatic Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 30 اردیبهشت 1404   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2025.2009179.1811

نویسندگان

Andrei B. Borovkov؛ Irina N. Gudvilovich؛ Anna L. Avsiyan^* ؛ Svetlana Yu. Gorbunova

Department of Biotechnology and Phytoresources, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Sevastopol, Russia

چکیده

The growth of red marine microalga Porphyridium purpureum and the production of B-phycoerythrin (B-PE) pigment were examined during pilot cultivation in a laboratory and in a greenhouse. The studies of P. purpureum cultivation in a greenhouse were conducted under mid-latitude climate conditions during different seasons. Maximum B-PE content (56 mg·g-1) and productivity (655.2 mg·m-2·day-1) were observed under controlled laboratory conditions. A strong linear correlation was found between the total daily irradiance and areal biomass productivity across different seasons. The total biomass and B-PE yield in May were significantly higher compared to other seasons (49.6 and 1.13 g·m-2), and in December these values were minimal. Despite the highest biomass productivity (12 g·m-2·day-1), culture death occurred in June. The photosynthetic efficiency exhibited seasonal variation, ranging from 2.87% in May to 3.83% in November, and was significantly higher under laboratory conditions (6.94%). The spring period is generally favorable for P. purpureum growth under mid-latitude climate conditions, whereas culture growth in summer has a certain potential, however, the impact of high illumination and temperature conditions should be considered and controlled. Although productivity in autumn-winter period was lower and limited by light supply, the possibility of obtaining additional biomass yield was demonstrated.

کلیدواژه‌ها

Porphyridium . Open ponds . Productivity . Yield . Phycobiliproteins . B؛ phycoerythrin