Interaction between probiotic bacteria and endemic microalgae in Japanese oyster larvae (Crassostrea gigas)

International Aquatic Research

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 09 خرداد 1404   

نوع مقاله: Original research

شناسه دیجیتال (DOI): 10.22034/iar.2025.2009290.1839

نویسندگان

Macario Savin-Amador^{* 1}؛ Sarai Guadalupe Flores González²؛ Maurilia Rojas-Contreras²؛ Giovanni Ávila Flores³؛ Ricardo Vázquez Juárez⁴

¹Coordinación de Ingenierías, Universidad Tecnológica de La Paz, C.P. 23088, Baja California Sur, Mexico; Laboratorio de Ciencia y Tecnología de Alimentos, Universidad Autónoma de Baja California Sur, C.P. 23085, Baja California Sur, Mexico

²Laboratorio de Ciencia y Tecnología de Alimentos, Universidad Autónoma de Baja California Sur, C.P. 23085, Baja California Sur, Mexico

³GII: Soluciones Basadas en la Naturaleza (SbN), Universidad Autónoma de Baja California Sur, C.P. 23085, Baja California Sur, Mexico

⁴Laboratorio de Genómica, Centro de Investigaciones Biológicas del Noroeste, S.C., C.P. 23205, Baja California Sur, Mexico

چکیده

Oysters are among the most widely cultivated aquatic species due to their economic and ecological importance. However, hatchery production of Japanese oyster (Crassostrea gigas) faces critical challenges, particularly high larval mortality caused by opportunistic bacterial infections and suboptimal feeding practices. In recent years, microalgae and probiotics have emerged as promising tools to improve larval performance and disease resistance, but their combined application remains insufficiently explored. This study evaluated the effects of native microalgae and the probiotic strain Lacticaseibacillus plantarum 69Cr on the survival of oyster larvae under controlled and pathogen-challenged conditions. The best performance was observed in the co-culture treatment of Schizochytrium sp. and Lpb. plantarum, both at a concentration of 10⁴ colony forming units per milliliter (CFU mL⁻¹), which resulted in a 37.5% survival rate in the absence of the pathogen and 18.75% under challenge with Vibrio parahaemolyticus. These findings indicate a positive synergistic interaction that enhances larval resistance and overall viability. However, more than 50% of larvae showed early digestive evacuation in treatments with Schizochytrium sp., suggesting limitations in its digestibility during early larval stages. Despite this, the use of native probiotics and microalgae presents a sustainable alternative to reduce antibiotic use in hatchery systems. By incorporating functionally compatible microbial strains into feeding protocols, hatcheries may improve survival rates, reduce costs associated with auxiliary algal cultures, and minimize pathogen outbreaks. This study contributes novel insights into probiotic–microalgae interactions and supports the integration of locally adapted microbial tools in bivalve aquaculture as part of a broader strategy to enhance sustainability and productivity.

کلیدواژه‌ها

Effective dose؛ Lpb. plantarum؛ V. parahaemolyticus؛ Microalgae