Green synthesis of silver nanoparticles using a combination of Allium sativum and Peganum harmala aqueous extracts and evaluating its antibacterial activity

International Journal of Molecular and Clinical Microbiology

دوره 14، شماره 2، اسفند 2024، صفحه 2102-2113   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/ijmcm.2024.710222

نویسندگان

Masoumeh Karimi¹؛ Khosrow Chehri^{* 1}؛ Sajad Moradi²؛ Mohsen Shahlaei²

¹Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran

²Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

چکیده

The resistance of bacteria to antibiotics has significantly increased due to their widespread and improper use. The emergence of multidrug-resistant (MDR) strains has made the treatment of infections more challenging, resulting in higher healthcare costs and mortality rates. This issue underscores the urgent need to develop alternative therapeutic approaches. This research was carried out in order to biosynthesis and identify silver nanoparticles using the combination of Allium sativum and Peganum harmala plant extracts and to evaluate their antibacterial effect.The characterization of the biosynthesized silver nanoparticles was performed using methods dynamic light scattering )DLS(, visible-ultraviolet spectroscopy (UV-Vis), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Energy-Dispersive X-ray (EDAX). The antimicrobial function of biosynthesized AgNP was also investigated using microdilution method against pathogenic Gram-positive Bacteria Staphylococcus aureus and Gram-negative bacteria, Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa. The results were finally analyzed using SPSS software. The SEM image shows spherical nanoparticles with a size of 40 to 45 nanometers. The biosynthesized nanoparticles show significant antibacterial effect, so that their minimum inhibitory concentration on Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhimurium and Escherichia coli were reported to be 62.5, 15.7, 31.25 and 62.5 ppm respectively (p≤0 / 05). According to the obtained results, silver nanoparticles prepared using mixed plant extracts of Allium sativum and Peganum harmala have a significant antimicrobial effect on Gram- negative and positive bacteria therefore can act as natural antimicrobial compounds.

کلیدواژه‌ها

Green synthesis؛ Silver nanoparticles؛ Allium sativum؛ Peganum harmala؛ Antimicrobial effect