Rapid Detection of Escherichia coli and Klebsiella pneumoniae in Urinary Tract Infections Using Multiplex PCR assay

International Journal of Molecular and Clinical Microbiology

دوره 14، شماره 2، اسفند 2024، صفحه 2122-2129   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/ijmcm.2024.710245

نویسندگان

Mina Behnamfard^{* 1}؛ Mahboobe Nakhaemoghaddam²؛ Negin Mortezaee³؛ Mobina Honarmandkhalilabad³

¹Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

²- Associate Professor, Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

³MSc of Microbiology, Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad

چکیده

Escherichia coli and Klebsiella pneumoniae predominantly cause urinary tract infections (UTIs). Rapid and accurate identification of these pathogens is crucial for effective treatment. This study aimed to evaluate multiplex PCR for the simultaneous detection of E. coli and K. pneumoniae in urinary samples. Conducted in 2023 at Ghaem Hospital, Mashhad, Iran, 250 urine samples from suspected UTI patients were collected. DNA was extracted using a commercial kit and amplified using specific primers (EC-16S for E. coli and KP/khe for K. pneumoniae). Results were compared with culture methods. Among the 250 samples, 110 were positive: 61 E. coli, 49 K. pneumoniae, and 12 co-infections. Multiplex PCR showed high sensitivity and specificity with faster turnaround than culture. It is a reliable and rapid diagnostic tool for UTIs. Multiplex PCR offers a rapid, sensitive, and specific alternative to culture for UTI diagnosis, enabling timely treatment decisions.

کلیدواژه‌ها

E.coli؛ K.pneumoniae؛ UTI؛ Multiplex PCR؛ Rapid diangosis؛ Culture؛ Molecolar method