Investigating the effect of prebiotic polysaccharides from algae and mushrooms on probiotic activity

International Journal of Molecular and Clinical Microbiology

دوره 15، شماره 1، مهر 2025، صفحه 2158-2179   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/ijmcm.2025.2009001.1309

نویسندگان

Amir Reza Rezaei¹؛ Mohaddeseh Larypoor^{* 2}؛ Mozhgan Emtiazjoo³

¹Department of Biotechnology, Faculty of Biological Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran

²Dep. Microbiology, Facuity of Biological science, Islamic Azad University,Tehran north Branch,Tehran,Iran

³Department of marine biology, Islamic Azad University Tehran North branch

چکیده

For human food safety, fungal or algal sources are used instead of plant sources. The aim of this research is to investigate the effects of prebiotic polysaccharides from Ganoderma, Lentinula, various fungi, Spirulina, Chlorella, and other algae on probiotics isolated from water and soil samples. Samples were collected from different traditional dairies. Microscopic, macroscopic, and biochemical tests, along with molecular methods, were used to identify bacterial and yeast isolates. Isolated strains were evaluated for their resistance to heat, acid, bile salts, and antibiograms to assess their probiotic potential. Polysaccharides were extracted from algae and fungi using the phenol-sulfuric acid method. The amount of polysaccharides extracted was then measured, and their structures were identified using FT-IR analysis. Subsequently, the effects of polysaccharides on the growth and postbiotic production of probiotic isolates were investigated to assess potential synergistic effects. Technical abbreviations will be explained when first introduced. Four strains—Pichia sp. strain HBUM07161, Saccharomyces cerevisiae strain CEN.PK113-7D, Lactobacillus gallinarum strain ATCC 33199, and Loigolactobacillus coryniformis—were identified during laboratory and molecular tests for their probiotic properties. The polysaccharides extracted from Ganoderma, Lentinula, Spirulina, and Chlorella algae had a stronger impact on growth rate and organic acid production as postbiotics compared to inulin, a standard prebiotic. The incorporation of biopolymers derived from algae and moss as prebiotics can inhibit the reduction of plant-based food sources. Moreover, due to their biocompatibility and non-toxicity, they provide suitable growth substrates for probiotics such as Ganoderma lucidum, Lentinula edodes, Spirulina, and Chlorella.

کلیدواژه‌ها

Biological prebiotic؛ Ganoderma lucidum؛ Lentinula edodes؛ Spirulina؛ Chlorella