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Lak Hondori, Masoumeh, Mohammadi-Sichani, maryam, Ghiasian, Mozhgan. (1404). Frequency of Beta-lactamase Genes (blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48) in Multidrug-resistant Klebsiella pneumoniae. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 15(1), 2180-2189. doi: 10.22034/ijmcm.2025.710258
Masoumeh Lak Hondori; maryam Mohammadi-Sichani; Mozhgan Ghiasian. "Frequency of Beta-lactamase Genes (blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48) in Multidrug-resistant Klebsiella pneumoniae". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 15, 1, 1404, 2180-2189. doi: 10.22034/ijmcm.2025.710258
Lak Hondori, Masoumeh, Mohammadi-Sichani, maryam, Ghiasian, Mozhgan. (1404). 'Frequency of Beta-lactamase Genes (blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48) in Multidrug-resistant Klebsiella pneumoniae', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 15(1), pp. 2180-2189. doi: 10.22034/ijmcm.2025.710258
Lak Hondori, Masoumeh, Mohammadi-Sichani, maryam, Ghiasian, Mozhgan. Frequency of Beta-lactamase Genes (blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48) in Multidrug-resistant Klebsiella pneumoniae. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1404; 15(1): 2180-2189. doi: 10.22034/ijmcm.2025.710258

Frequency of Beta-lactamase Genes (blaSHV, blaTEM, blaCTX-M-15, blaKPC, blaOXA-48) in Multidrug-resistant Klebsiella pneumoniae

International Journal of Molecular and Clinical Microbiology
دوره 15، شماره 1، مهر 2025، صفحه 2180-2189    اصل مقاله (734.87 K)
نوع مقاله: Research Article
شناسه دیجیتال (DOI): 10.22034/ijmcm.2025.710258
نویسندگان
Masoumeh Lak Hondori؛ maryam Mohammadi-Sichani* ؛ Mozhgan Ghiasian
Department of Microbiology, Fal. C., Islamic Azad University, Isfahan, Iran
چکیده
The global spread of multidrug-resistant (MDR) strains of Klebsiella pneumoniae is a significant public health concern. This study aims to investigate the prevalence of beta-lactamase genes blaSHV, blaTEM, blaCTX-M-15, blaKPC, and blaOXA-48 in MDR K. pneumoniae isolates obtained from nosocomial infections in Isfahan, Iran. Ninety-six K. pneumoniae isolates were collected from clinical infections. After confirming the identity of the isolates through biochemical tests, their antibiotic sensitivity was assessed using the disk diffusion method. Extended-spectrum beta-lactamase production was evaluated with the combination disk method, utilizing cefotaxime and cefotaxime/clavulanic acid. The presence of beta-lactamase genes was detected using specific primers and PCR. All isolates were resistant to amoxicillin. Additionally, resistance was observed in 56.25% of isolates to imipenem, 57.29% to cephalothin, 52.08% to ciprofloxacin, and 52.08% to cefotaxime. Conversely, the highest sensitivity was recorded for tetracycline (64.58%), followed by chloramphenicol (61.45%), gentamicin (56.25%), and nitrofurantoin (54.16%). Phenotypic testing revealed that all 96 isolates were positive for broad-spectrum beta-lactamases. Moreover, 44 isolates were classified as MDR, exhibiting resistance to at least three different antibiotic classes. The frequencies of the blaKPC, blaOXA-48, blaCTX-M-15, blaTEM, and blaSHV genes among MDR strains were 45.45%, 50%, 72.72%, 84.09%, and 86.36%, respectively. The presence of MDR isolates and broad-spectrum beta-lactamase genes, particularly in K. pneumoniae, highlights the need for cautious management of these infections.
کلیدواژه‌ها
Klebsiella؛ Multidrug resistant؛ antibiotic resistance؛ beta lactamase؛ Polymerase chain reaction
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