Identification and Antifungal Susceptibility Testing of Candida species isolated from Bronchoalveolar Lavage sampl

International Journal of Molecular and Clinical Microbiology

مقاله 4، دوره 4، شماره 1، شهریور 2014، صفحه 358-364   

نوع مقاله: Research Article

نویسندگان

Mojtaba Taghizadeh Armaki¹؛ Mohammad Taghi Hedayati^{* 2}؛ Saeed Mahdavi Omran³؛ Sasan Saber⁴؛ Mahdi Abastabar²؛ Akbar Hosseinnejad¹

¹Department of Medical Mycology and Parasitology, Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

²Invasive Fungi Research Center/ Department of Medical Mycology and Parasitology, Mazandaran University of Medical Sciences, Sari, Iran

³Department of Parasitology and Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran,

⁴Department of Internal Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

چکیده

The frequency of fungal infections in immunocompromised patients, particularly by Candida species, has increased in recent years. Colonization by Candida species in respiratory tract in susceptible hosts may play an important role to precede disseminated candidiasis. This study was designed to identify Candida species from bronchoalveolar lavage (BAL) samples and determination of antifungal susceptibility of isolates to Ketoconazole, Clotrimazole, Fluconazole and Nystatin by disk diffusion method. Sampling was conducted between from 2011 to 2014 years. Three hundred and eighty four patients who were suspected to invasive fungal infections were enrolled in the study. Clinical specimens were studied for direct microscopic examination and culture. The antifungal activity test for Candida species isolated from BAL samples was performed by using disk diffusion, according to CLSI documents M44-A2. Eighty seven (%22.66) patients showed the symptoms, signs and predisposing factors for pulmonary fungal infections. The isolated species were identified as follows:  C.albicans, 31 (67.39%); C.glabrata, 9 (19.56 %); C.krusei, 3 (6.5%); C.parapsilosis, 2 (4.3%); and C.tropicalis , 1 (2.25%). In this study, resistance to antifungal agents were seen to Ketoconazole, 2 (4.38%),Clotrimazole 1 (2.17%) andFluconazole, 4 (8.69%). Determination of antifungal sensitivity of the isolated yeast species should be the basis of rational and successful therapy.

کلیدواژه‌ها

Antifungal Susceptibility Testing؛ Candida؛ Bronchoalveolar Lavage samples