Appraisal of Hematological Indices and Humoral Immunity in Commercial Laying Hens Fed Rations Consisting Cottonseed Meal and Sodium Bentonite

Iranian Journal of Applied Animal Science

مقاله 22، دوره 3، شماره 4، اسفند 2013، صفحه 803-809   

نوع مقاله: Research/Original Article

نویسندگان

A. Gilani^* ؛ H. Kermanshahi؛ A. Golian؛ A. Tahmasbi؛ M. Aami Azghadi

Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

The objective of this study was to evaluate the effects of cottonseed meal (CSM) treated with sodium bentonite (SB) on certain blood components and immune response of laying hens. A 3 × 3 factorial arrangement with 3 levels of SB (0, 10, and 20 g/kg of diet) and 3 levels of CSM (0, 100 and 200 g/kg of diet) were used within 9 dietary treatments of 4 replicates each. Nine mash diets were fed to 288 commercial Hy-Line W-36 hens from 51-63 weeks of age. Blood samples were collected at the end of trial to evaluate the blood constituents. The SB, CSM, or their interaction (SB×CSM) did not have any adverse effect on blood cells and activity of serum enzymes. Sheep red blood cells (SRBC) were used as antigen to quantify the antibody response. Two birds per replicate were injected with SRBC at 60 week of age. After 7 and 14 d of SRBC inoculation, blood samples were obtained from the brachial vein of each hen, and total anti-SRBC, IgG and IgM titers were determined. A significant difference of SB, CSM or their interaction was not observed for total antibody response against SRBC inoculation; however, IgG was significantly increased with 20% CSM at 7 d (7.62 vs. 8.45) and with 2% SB at 14 d (2.79 vs. 3.66) after injection of SRBC. The interaction of SB and CSM for IgG was significantly different among dietary treatments and diet with 2% SB and 20% CSM had the highest titer (5 vs. 2.5) at 14 d after injection of SRBC. In conclusion, SB, CSM or their interactions did not affect blood constituents but significantly changed immune response of the birds.

کلیدواژه‌ها

blood components؛ cottonseed meal؛ humoral immunity؛ laying hens؛ sodium bentonite

مراجع

Akbarian A., Golian A., Kermanshahi H., Gilani A., and Moradi S. (2012). Influence of turmeric rhizome and black pepper on blood constituents and performance of broiler chickens. African J. Biotechnol. 11, 8606-8611.
AOAC. (1996). Official Methods of Analysis. Vol. I. 16^th Ed. Association of Official Analytical Chemists, Arlington, VA.
Araba M. and Wyatt R.D. (1991). Effects of sodium bentonite hydrated aluminosilicate (Novasil) and charcoal on aflatoxicosis in broiler chickens. Poult. Sci. 70, 6-11.
Atuaheme C.C., Donkeh A. and Nkhash Darko A. (1986). Effect of raw cottonseed meal on the performance, carcass characteristics and certain blood parameters of broiler chickens. J. Anim. Prod. Res. 6, 107-114.
Cheema M.A., Qureshi M.A. and Havenstein G.B. (2003). A comparison of the immune response of a 2001 commercial broiler with a 1957 randombred broiler strain when fed representative 1957 and 2001 broiler diets. Poult. Sci. 82, 1519-1529.
Danke R.J. and Tilman A.D. (1966). Effect of free gossypol and supplemental dietary iron on blood constituents of rats. J. Nutr. 87, 493-498.
Emadi M. and Kermanshahi H. (2007a). Effect of turmeric rhizome powder on immunity responses of broiler chickens. J. Anim. Vet. Adv. 6, 833-836.
Emadi M. and Kermanshahi H. (2007b). Effect of turmeric rhizome powder on the activity of some blood enzymes in broiler chickens. Int. J. Poult. Sci. 6, 48-51.
Ferguson T.M., Couch J.R.and Rigdon R.H.(1959).Histopathology ofanimal reactions to pigment compounds-chickens. Pp. 131-141 in Proc.Chem. Struc. Reacti.  Gossyp. Non-gossyp. PigmentsCottonseed, Nati. Cottonseed Prod.Assoc. Conf., Memphis, Tennessee.USA.
González Garza M.T., Montalva I., Mota L. and Sotelo A. (1986). Lactic dehydrogenase activity in various organs of rats fed cottonseed flour. Nutr. Res. 6, 443-449.
Hasan G., Toloei T. and Habibi M. (2010). Efficacy of esterified glucomannan, sodium bentonite and humic acid to counteract experimental aflatoxicosis on antibody titers against Newcastle disease in broilers. African J. Biotechnol. 9, 4127-4131.
Hashemipour H., Kermanshahi H. and Pilevar M. (2010). Interactive effect of sodium bentonite with pigments on performance and egg quality of laying hens. J. Anim. Vet. Adv. 9, 2179-2184.
Henry M.H., Pesti G.M., Bakalli R., Lee J., Toledo R.T.andPhillips R.D.(2001). The performance of broiler chicksfed diets containing extruded cottonseed mealsupplemented with lysine. Poult.Sci. 80,762-768.
IbrahimI.K., Shareef A.M. and Al-Joubory K.M.T. (2000). Ameliorative effects of sodium bentonite on phagocytosis and Newcastle disease antibody formation in broiler chickens during aflatoxicosis. Res. Vet. Sci. 69, 119-122.
ISIRI. (1997). Determination of free and total gossypol in animal feedstuffs. ISO 6866. ISIRI number 3022.
Jalees M.M., Khan M.Z., Saleemi M.K.and Khan A.(2011). Effects of cottonseed meal onhematological, biochemical and behavioral alterations in male Japanese quail (Coturnix japonica). PakistanVet.J. 31, 211-214.
Kermanshahi H., Hazegh A.R. and Afzali N. (2009). Effect of sodium bentonite in broiler chickens fed diets contaminated with aflatoxin B1. J. Anim. Vet. Adv. 8, 1631-1636.
Koenen M.E., Boonstra Biom A.G. and Jeurissen S.H.M. (2002). Immunological differences between layer and broiler-type chickens. Vet. Immunol. Immunopathol. 89, 47-56.
Kubena L.F., Harvey R.B., Huff W.E., Elissalde M.H., Yersin G.A., Phillips T.D. and Rottinghaus G.E. (1993). Efficacy of a hydrated sodium calcium aluminosilicate to reduce the toxicity of aflatoxin and diacetoxyscirpenol. Poult. Sci. 72, 51-59.
Lordelo M.M., Calhoun M.C., Dale N.M., Dowd M.K. and Davis A.J. (2005). Relative toxicity of gossypol enantiomers in broilers. Poult. Sci. 84, 1376-1382.
Lordelo M.M., Calhoun M.C., Dale N.M., Dowd M.K. and Davis A.J. (2007). Relative toxicity of gossypol enantiomers in laying and broiler breeder hens. Poult. Sci. 86, 582-590.
Mahmood F. (2006). Cottonseed meal induced pathology in broiler breeder males. Ph D. Thesis. Univ. Agric. Faisalabad, Pakistan.
Mandal A.B., Elangovan A.V. and Verma S.V.S.(2001).Interactionof nutrition and infection in poultry:areview. Anim. Nutr. Feed Technol. 1, 19-38.
Miazzo R., Peralta M.F., Magnoli C., Salvano M., Ferrero S., Chiacchiera S.M., Carvalho E.C., Rosa C.A.andDalcero A. (2005). Efficacy of sodium bentonite as a detoxifier of broiler feed contaminated with aflatoxin and fumonisin. Poult. Sci. 84, 1-8.
Nagalakshmi D., Rama Rao S.V., Panda A.K. and Sastry V.R.B. (2007). Cottonseed meal in poultry diets: a review. J. Poult. Sci. 44, 119-134.
Phillips T.D., Kubena L.F., Harvey R.B., Taylor D.R. and Heidelbaugh N.D. (1988). Hydrated sodium calcium aluminosilicate: a high affinity sorbent for aflatoxin. Poult. Sci. 67, 243-247.
Qureshi M.A. and Havenstein G.B. (1994). A comparison of the immune performance of a 1991 commercial broiler with a 1957 randombred strain when fed ‘typical’ 1957 and 1991 broiler diets. Poult. Sci. 73, 1805-1812.
Safaeikatouli M., Jafariahangari Y. and Baharlouei A. (2010). Effects of dietary inclusion of sodium bentonite on biochemical characteristics of blood serum in broiler chickens. Int. J. Agric. Biol. 12, 877-880.
Saqib K.M., Rahman Z.U. and Nagra S.A. (2012). Effect of weightlessness on neutrophils and lymphocytes of rats. Pakistan Vet. J. 32, 25-30.
Salari S., Kermanshahi H. and Nasiri Moghaddam H. (2006). Effect of sodium bentonite and comparison of pellet vs. mash on performance of broiler chickens. Int. J. Poult. Sci. 5, 31-34.
SAS Institute. (2004). SAS^®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC.
Skutches C.L., Herman D.C. and Smith F.H. (1973). Effect of I / V gossypol injection on iron utilization in swine. J. Nutr. 103, 851-855.
Tang J.W., Sun H., Yao X.H., Wu Y.F., Wang X.and Feng J.(2012). Effects of replacement of soybean meal by fermented cottonseed meal on growth performance, serum biochemical parameters and immune function of yellow-feathered broilers. Asian-australasJ. Anim. Sci. 25,393-400.
Trckova M., Matlova L., Dvorska L. and Pavlik I. (2004). Kaolin, bentonite and zeolites as feed supplements for animals: health advantages and risks. Vet. Med. Czech. 49, 389-399