Genotypic and Allelic Frequencies of IGF1 and IGF2 Genes in Broilers Analysed by Using PCR‐RFLP Techniques

Iranian Journal of Applied Animal Science

مقاله 9، دوره 2، شماره 4، اسفند 2012، صفحه 357-360   

نویسندگان

T. Shah¹؛ S. Deshpande^{* 2}؛ U. Dasgupta²؛ K.M. Singh³؛ C.G. Joshi¹

¹Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India

²Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India

³Department of Genetics, ARIBAS, New V.V. Nagar, Anand, India

چکیده

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 butA-allele (0.5611) IGF2 in the broiler population.

کلیدواژه‌ها

genotype؛ IFG1؛ IGF2؛ PCR-RFLP

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