Polymorphisms of Candidate Genes for Muscle Perform and Male Fertility in Brazilian Mangalarga Horses

Iranian Journal of Applied Animal Science

مقاله 15، دوره 2، شماره 2، شهریور 2012، صفحه 199-202   

نوع مقاله: Short Communication

نویسندگان

L.C.M. Armeiro¹؛ R.A. Curi²؛ L.A.L. Chardulo³؛ J.N.P. Puoli Filho²؛ M.D. Silveira da Mota^{* 2}

¹Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP 14884-900, Brasil

²Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brasil

³Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP 18618-000, Brasil

چکیده

The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, in Mangalarga horses, in order to provide a basis for future studies investigating the association between markers and traits of interest in this breed. For this, 151 Mangalarga horses of both sexes, representatives of the population of the Sate of São Paulo, Brazil, were used. Polymerase chain reaction-restriction fragment length polymorphismPCR-RFLP was found to be adequate for the genotyping of the AY_376689:c.773C>T single nucleotide polymorphismSNP of the PRKAG3 gene. However, this polymorphism probably does not segregate in Mangalarga horses, a fact impairing association studies with muscle performance traits. The estimates of population genetic parameters obtained for the AAWR_02017454:g.121684T>C polymorphism of the equine SPATA1 gene demonstrated the possibility of association studies between this marker and traits related to male fertility.

کلیدواژه‌ها

Mangalarga horses؛ PRKAG3؛ SNP؛ SPATA1

مراجع

Barendse W.J. (2005). The transition from quantitative trait loci to diagnostic test in cattle and other livestock. Australian J. Exp. Agr. 45, 831-836.
Binns M.M., Boehler D.A. and Lambert D.H. (2010). Identification of the myostatin locus (MSTN) as having a major effect on optimum racing distance in the Thoroughbred horse in the USA. Anim. Genet. 41(2), 154-158.
Giesecke K., Hamann K., Stock K.F., Woehlke A., Sieme H. and Distl O. (2009). Evaluation of SPATA1- associated markers for stallion fertility. Anim. Genet. 40, 359-365.
Hill E.W., Gu J., McGivney B.A. and MacHugh D.E. (2010). Targets of selection in the Thoroughbred genome contain exercise-relevant gene SNPs associated with elite racecourse performance. Anim. Genet. 41(2), 56-63.
LancasterA.K., Single R.M., Solberg O.D., Nelson M.P. and Thomson G. (2007). PyPop update-a software pipeline for large-scale multilocus population genomics. Tissue Antig. 69,192-197.
Park H.B., Marklund S., Jeon J.T., Mickelson J.R., Valberg S.J., Sandberg K. and Andersson L. (2003). Molecular characterization and mutational screening of the PRKAG3 gene in the horse. Cytogenet.Genome Res. 102, 211-216.
Sambroock J., Fritsch E.F. and Maniatis T. (1989). Molecular Cloning: A Laboratory Manual. 2^nd Ed. Cold Spring Harbor Laboratory Press, New York, USA.
Slatkin M. (1996). A correction to the exact test based on the Ewens sampling distribution. Genet. Res. 68, 259-260.
Tozaki T., Miyake T., Kakoi H., Gawahara H., Sugita S., Hasegawa T., Ishida N., Hirota K. and Nakano Y. (2010). A genome-wide association study for racing performances in Thoroughbreds clarifies a candidate region near the MSTN gene. Anim. Genet. 41(2), 28-35.
Van Eenennaam A.L., Li J., Thallman R.M., Quaas R.L., Dikeman M.E., Gill C.A., Franke D.E. and Thomas M.G. (2007). Validation of commercial DNA tests for quantitative beef quality traits. J. Anim. Sci. 85, 891-900.
Yeh F.C., Yang R.C. and Boyle T. (1999). Popgene version 1.32. Microsoft Windows-Based Freeware for Population Genetic Analysis, University of Alberta, Canada.