Studies on the Antioxidant and Antimicrobial Activity and Flavonoid Derivatives from Fruits of Trigonosciadium brachytaenium (Boiss.) Alava.

Journal of Pharmaceutical & Health Sciences

مقاله 1، دوره 2، شماره 3، شهریور 2014، صفحه 89-95   

نوع مقاله: Original Article

چکیده

The objective of this study was to evaluate the potential use of the fruits of Trigonosciadium brachytaenium
in the pharmaceutical and food industries. Flavonoids are present in food and medicinal plants
and are thus consumed by humans. They are found in plants as glycosides. Their biological activities
have an impact on human health so that they serve as target molecules to develop new drugs. From
methanolic extract of fruits of Trigonosciadium brachytaenium (Boiss.) Alava. (Umbelliferae
family), two flavonoid derivatives namely 5- Hydroxy- 3’-methoxy-4’- ethoxyflavone-7-O-(2’’-(4’’’-
acetyl-rha)-rha)(1) and 5- Hydroxy- 4’-methoxy-8- ethoxyflavone-7-O-(2’’-(2’’’- acetyl-rha)-rha)(2)
have been isolated by column chromatography (CC) and preparative TLC (PTLC). Those structures
were elucidated by UV, 1H- and 13C- NMR, HMBC, EI-MS and IR spectra. The antioxidant activity
of methanol extract was evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method. The results
indicate that methanol extract from aerial parts of T. brachytaenium possess considerable antioxidant
activity. The highest radical scavenging activity was detected (IC50 = 47 μg/mL). This study reveals
that the methanolic extract of this plant is attractive sources of flavonoid, especially the essential
ones, as well as of effective natural antioxidants. The antimicrobial activity of the methanol extract
of aerial part was determined against seven Gram-positive and Gram-negative bacteria (Bacillus
subtilis, Enterococcus faecalis, Staphylococcus aureus, S. epidermidis, Escherichia coli, Pseudomonas
aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces
cerevisiae and Aspergillus niger). The bioassay showed that the extract exhibited good antimicrobial
activity. These flavonoid compounds were isolated for the first time from T. brachytaenium.

کلیدواژه‌ها

Trigonosciadium brachytaenium؛ Umbelliferae؛ methanolic extract؛ flavonoid؛ Antimicrobial Activity؛ Antioxidant Activity