Polymorphism of some Genes Associated with Meat-Related Traits in Egyptian Sheep Breeds

Iranian Journal of Applied Animal Science

مقاله 19، دوره 5، شماره 3، آذر 2015، صفحه 655-663   

نوع مقاله: Research/Original Article

نویسندگان

K.F. Mahrous¹؛ M.S. Hassanane¹؛ M. Abdel Mordy²؛ H.I. Shafey^{* 1}؛ H.E. Rushdi³

¹Department of Cell Biology, National Research Center, Dokki, Giza, Egypt

²Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt

³Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt

چکیده

The genetic polymorphism of some genes related to meat production in three Egyptian sheep breeds (Barki, Rahmani and Osseimi) was studied. The candidate genes were: Calpastatin, Myostatin, Diacylglycerol-acyltransferase1, Insulin-like growth factor binding protein-3 and Booroola fecundity gene. The technique applied was the restriction fragment length polymorphism for the polymerase chain reaction products. Polymorphism was found in the genes: Calpastatin, MyostatinandDiacylglycerol-acyltransferase 1, while no polymorphism was exhibited by the other two genes, Insulin-like growth factor binding protein-3 and the Booroola fecundity in the three breeds understudy. Calpastatin locus digested with MspI had two genotypes MM and MN. The highest allelic frequency was for allele M. The same locus Calpastatin digested with NcoI also exhibited two genotypes MM and MN. The NN genotype was absent with both the MspI and the NcoI enzymes in all breeds. Myostatin digested with DraI had two genotypes AB and BB. The AA genotype cannot be detected. The highest allelic frequency was for allele B. Diacylglycerol-acyltransferase1 digested with AluI showed two genotypes CC and CT. The highest allelic frequency was for allele C. The detected CT genotype might explain the moderate intramuscular fat content and muscle marbling in the Egyption sheep breeds. Each of the remaining two loci (Insulin-like growth factor binding protein-3 and the Booroola fecundity) had only one genotype, BB genotype for Insulin-like growth factor binding protein-3 digested with HaeIII and ++ genotype for the Booroola fecundity digested with AvaII enzyme, therefore they are not recommended in the selection program. The result of Chi-square analysis indicated that the three Egyptian sheep breeds were in Hardy-Weinberg equilibrium.

کلیدواژه‌ها

genetic polymorphism؛ PCR-RFLP؛ Sheep

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