Investigation of Chilling Effects on Characteristics of Seed Germination, Vigor and Seedling Growth of Nepeta spp. Species

Journal of Rangeland Science

مقاله 8، دوره 5، شماره 4، دی 2015، صفحه 313-324   

نویسندگان

Marzieh Asgari^{* 1}؛ Mohsen Nasiri²؛ Ali Ashrafe Jafari³؛ Leila Flah Hoseini⁴

¹M.Sc. Rangeland Management, Young Researchers and Elite Club, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

²Research Institute of Forests and Rangelands, Tehran, Iran

³Professor, Research Institute of Forests and Rangelands, Tehran, Iran

⁴M.Sc. Student, Department of Agronomy and Plant Breeding, Islamic Azad University, Karaj Branch, Karaj, Iran

چکیده

To study the effects of chilling on dormancy breaking and the increased germination characteristics of Nepeta (germination percent and speed, seedling length, vigor index, wet and dry weight), a factorial experiment in a completely randomized design with 4 replications was implemented in a laboratory in 2014. Factor A was the chilling treatment in three levels (control, one-month and two-month pre-chilling of wet seeds at 4°C) and factor B was related to the seeds of 10 different species of genus Nepeta. After chilling, seeds of control were placed in a germinator for 15 days at 20°C and light-to-dark cycle of 16 hours light (1000 lux) to 8 hours dark. The results showed that chilling treatments had significant effects (p<0.01) on germination percent, germination speed, vigor index and fresh weight, but they had no effects on the seedling length. The chilling effects on germination percent showed that one-month chilling had a positive effect on N. haussknechtii with 40% and N. menthoides with 36.5% and two-month chilling had a positive effect on N. haussknechtii with 43%. The two-month chilling effect on germination speed was positive for most species. Generally, one-month chilling had positive effects on the increased seed vigor index of N. betonicifolia, N. haussknechtii  and N. menthoidesand by 20.48%, 25.33% and 17.99%, respectively as compared to that of control treatment and two-month chilling had positive effects on the increased fresh weight of N. cataria, N. haussknechtii , N. pungens, N. menthoides andN. crassifolia.

کلیدواژه‌ها

chilling؛ Nepeta؛ Dormancy breaking؛ germination

مراجع

Abdul-baki, A. A. and Anderson, J. D., 1973. Vigor determination in soybean seed by multiplication. Crop Sci., 3: 630-633.

Alizadeh, M. A. and Jafari, A. A., 2010. The effect of cold treatment on germination characteristics and vegetative traits in five ecotypes of Dactylis glomerata in germinator and greenhouse. Iranian Jour. Range Desert, 17(1): 115-126. )In Persian(.

Amin, G. R., 1991. Popular medicinal plants of Iran. Ministry of Health Pub. Tehran., 1: 40-41. )In Persian(.

Aydin, S., Besi, R., Ozturk, Y. and Baser, K. H. C., 1998. Nepeta lactones a new opioid analgesic from Nepeta caesarea Boiss. Jour. Pharmacol., 50: 813-817.

Baskin, C. C. and Baskin, J. M., 1999. Seed ecology, dormancy and germination. A modern synthesis. Am. Jour. Botany, 86: 903-905.

Bewley, J. D., 1997. Seed germination and dormancy. Plant Cell. 9: 1055-1066.

Evans, W. C., 1996. Trease and Evans pharmacogonosy. London: W. B. Saunders Company. p. 48.

Fakhr Ranjbari, H., 1997. The investigation of chemical components in essential oil of N. asterotricha Rech., M.Sc. thesis. Tehran teacher training university. )In Persian(.

Ghannadi, A., Aghazari, F., Mehrabani, M., Mohagheghzadeh, A. and Mehregan, I., 2003. Quantity and composition of the SDE prepared essential oil of Nepeta macrosiphon Bioss. Iranian Jour., Pharmaceutical Research, 5: 103-105. )In Persian(.

Graber, R. E., 1965. Germination of Eastern white pine seed as influenced by stratification, U.S. forest service research paper NE-36, 11pp.

Hosienpour Gazviniy, M., Alizadeh, A., Jafari, A. A. and Valadabadi, A. R., 2012. Effect of scarification, cold and after-ripening treatments on seed dormancy breaking in four species of Satureja by standard germination test. Iranian Jour. Medicinal Aromatic Plants, 28(1): 48-58. )In Persian(. 

Hidayati, S. N., Baskin. J. M. and Baskin, C. C., 2000. Morph physiological dormancy in seeds of two North American and one Eurasian species of Sambucus (caprifoliaceae) with under developed spatulate emboys. Am. Jour. Botany., 87: 1669-1678. )In Persian(.

Koornneff, M., Bentsink, L. and Hilhorst, H., 2002. Seed dormancy and germination. Growth and Development. 5: 33-36.

Leadem, C. L., 1997. Dormancy-Unlocking seed secrets In: Landis T. D., Thompson J. R. National Proceedings, Forest and Conservation Nursery Associations. Gen.­ Tech.­ Rep. Portland, Forest Service, Pacific North West Research Station: 43-52.

Maguire, J. D., 1962. Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Sci., 2: 176–177.

Modares Hashemi, M., 1995. Investigation on seed dormancy breaking and germination of Prangos ferulacea lindly. Pajouhesh- Sazandegi, 29: 34-40. )In Persian(.

Mozaffarian, V., 2006. A dictionary of Iranian plant names: Latin - English - Persian. 4^th Ed. Farhang Moaser. Tehran. P: 360. (In Persian).

Nasiri, M., 2014. An analysis of seed germination and dormancy breaking in some of the Thymus species growing in Iran. Plant Ecosystem, 40: 87-96. )In Persian(.

Nasiri, M., Babakhanloo, P. and Maddah-Arefi, H., 2003. First report on braking dormancy and seed germination on Diplotaenia damavandica Mozaffarian, Hedge & Lamond. Iranian Jour. Range Forests Breeding Genetic Research, 2: 258-274. (In Persian).

Pederson, L., Jqrgensen P. E. and Poulsen, I., 1993. Effect of seed vigour and dormancy on field emergence, development and grain yield of winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.), Seed Sci. Tech., 21: 159-178.

Perry, D. A., 1978. Report of the vigour test committee for 1974-1977. Seed Sci. Tech., 6: 151-181.

Rechinger, K. H., 1982. Flora Iranica. Akademische adruck-u. Verlagsanstalt, Graz, Austria, 150: 590p.

Skaltsa, H. D., Lazari, D. M., Loukisand, A. E., Constantinidis, T., 2000. Essential oil analysis of Nepeta argolica Bory & Chaub. Subsp. Argolica (Lamiaceae) growing wild in Greece. Flavour Fragr. Jour. 15: 96-99.

Skordilis, A. & Costas, A. T., 1995. Seed stratification and germination strategy in the Mediterranean pines Pinus brutia and Pinus halepensis, Seed Science Research, 5: 151-160.

Shariati, M., Asemaneh, T., Modares Hashemi, M., 2002. Effects of different treatments on seed dormancy of Achillea millefolium. Iranian Jour., Rangelands and Forests Plant Breeding and Genetic Research. 15: 2-8. )In Persian(.

Trui, K. and Okagami, N., 1993. Temperature effects on seed germination of East Asian and Tertiery relict species of Dioscorea (Dioscoreaceae). Am. Jour. Botany. 80: 493-499.

Widrelechner, M. P. and Kovach, D. A., 2000. Dormancy- breaking protocols for Cuphea seed. Seed Sci & Technol., 28: 11-27.

Yamauchi, Y., Ogawa, M., Kuwahara, A., Hanada, A., Kamiya, Y., Yamaguchi, S., 2004. Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. The Plant Cell., 16: 367–378.