Pathogenicity of Alternaria Species Isolated from Chamaecyparis lawsonia In Vitro

Journal of Ornamental Plants

مقاله 6، دوره 5، شماره 3، آذر 2015، صفحه 183-188   

نوع مقاله: Research Paper

نویسندگان

Mohammad Reza Safari Motlagh^{* 1}؛ Fatemeh Ramezani Rad²؛ Shahram Sedaghathoor³

¹Department of Plant Pathology, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Guilan Province, PO box 41335-3516, Iran

²Master of Horticulture, Rasht Branch, Islamic Azad University, Rasht, Iran

³Department of Horticulture, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran

چکیده

Chamaecyparis lawsonia, which brings beauty in parks and green space, is attacked by a variety of pathogenic agents specially fungi. In this study, some Chamaecyparis lawsonia available in parks that had disease symptoms were  sampled. After sample collection, in order to isolate fungal pathogens, cuts of the infected plant tissues were placed on PDA (potato dextrose agar) and later were put on WA (water agar) for identifying. Then test of pathogenicity of these isolates on Chamaecyparis lawsonia was done inside the box with plastic stopper. To do this, cuts of colonies of 4 day fungi obtained on PDA medium were put on leaves. The final evaluation was performed after 10 days. This experiment was performed in a completely randomized design with 3 replications and 7 treatments. The results showed that isolated fungi are in 2 groups as following: Alternaria franseriae and Alternaria tenuissima. According to the
results, both species were pathogenic on host. Totally, A. franseriae showed more disease severity compared to A. tenuissima on C. lawsonia. 

کلیدواژه‌ها

Alternaria spp؛ Chamaecyparis lawsonia؛ Fungi؛ pathogenicity

مراجع

Ankudinov, A.M. 1950. Causes of seedling mortality in forest nurseries and measures for controlling
them. Lesno Khozyaistvo. 5: 26-30.
Asdaghi, A., Rahimi Kakavand, N. and Asadi, I. 2014. Contamination of Populus euphratica bordering
the river Karun with fungus Alternaria alternata. The First National Conference on Agricultural
Pollution and Food Safety, Challenges and Solutions. Ahvaz. Ramin University of Khuzestan.
Barnard, E.L. 1999. Annosum root rot of pines in Florida. Plant Pathology Circular No. 398. (Abs.).
Barnard, E.L., Blakeslee, G.M., English, J.T., Oak, S.W. and Anderson, R.L. 1985. Pathogenic
fungi associated with sand pine root disease in Florida. Plant Disease. 69:196-199.
Barnard, E.L. and Meeker, J.R. 1995. Leptographiumroot infection of pines in Florida. Plant Pathologhy
Circular No. 369. (Abs.).
Chandrashekar, M. and Ball, M.C. 1980. Leaf blight of grey mangrove in Australia caused by Alternaria
alternata. Transactions of the British Mycological Society. 75:413-418.
Ellis, M.B. 1971. Dematiaceous hyphomycetes. CMI, Kew, England, 608 pp.
Ershad, J. 1978. Iran fungi. Publication No. 10, Plant Pests and Diseases Research Institute, 277 pages.
Green, S., Brasier, C.M., Schlenzing, A., McCracken, A., MacAkill, G.A., Wilson, M. and Webber,
J.F. 2013. The destructive invasive pathogen Phytophthora lateralis found on Chamaecyparis
lawsoniana across the UK. Forest Pathology. 43:19-28.
Herfehdoost, F., Rostami Shahrajy, D. and Khodaparast, A. 2009. Investigation of the causes of death
in nursery conifers in trees nursery of Lakan. Scientific Research Journal of Forest and Spruce
of Iran, 17(2): 263-271.
Huang, J. W. and Kuhlman, E. G. 1990. Fungi associated with damping-off of slash pine seedlings
in Georgia. Plant Disease. 74:27-30.
Jafarpour, B. 1994. Diseases of trees and field and laboratory manual (translated). Publication of
Mashhad. 335 p.
Kakalikova, L., Jankura, E. and Srobarova, A. 2009. First report of Alternaria bunch rot of grapevines
in Slovakia. Australasian Plant Disease Notes. 4:68-69.
Kamalakannan, A., Gopalakrishnan, C., Renuka, R., Kalpana, K., Ladha, D. and Valluvaparidasan,
V. 2008. First report of Alternaria alternata causing leaf spot on Aloe barbadensis in India.
Australasian Plant Disease Notes. 3:110-111.
Kamran, R. and Bani-Hashemi, Z. 1995. Study of the etiology of palm leaf spot in Fars and Bushehr.
Research and Science Quarterly of Plant Diseases of Iran, 31(1-4): 16-23.
Lesli, J.F. and Summerell, B.T. 2006. The Fusarium Laboratory Manual. Blackwell Publishing.
388pp.

Nag Raj, T.R. 1993. Coelomycetous anamorphs with appendage- bearing conidia. Mycology Publications
Waterloo Canada. 1101 pp.
Safari Motlagh, M.R. 2000. Etiology of brown spot disease in Guilan province. Master's thesis in plant
pathology, Tehran University. 93 p.
Safari Motlagh, M.R. 2011. Evaluation of Curvularia lunata as an biological control agent in major
weeds of rice paddies. Life Science Journal, 8(2):81-91.
Shanmugan, V., Dhyani, D. and Ananthapadmanaban, D. 2011. First report of Alternaria sp. causing
blight on Incarvillea emodi. Australasian Plant Pathology Society Inc. 6:33-35.
Simmons, E.G. 2007. Alternaria, an identification manual. CBS Fungal Biodiversity Center,Utrecht,
the Netherlands. 775 p.
Soleimani, M.J. and Esmailzadeh, M. 2007. First report of Alternaria mali causing apple blotch
disease in Iran. Australasian Plant Disease Notes. 2:57-58.
Yousefi, A. and Hagian Shahri, M. 2009. Brown spot disease of peach and apricot trees, pathogenicity
and overwinter. Asian Journal of Plant Pathology. 3(3):61-69.
Zare, H. 2002. Native and non native species of conifers in Iran. Research and Forest-pasture Publications.
498 p.
Zarghani, H., Bani-Hashemi, R., Mostofizadeh, R. and Saadati, H. 2010. Identification of pathogenic
soil fungi in hand planting conifers in Fars province. Journal of Forestry and Wood-products.
Iranian Journal of Natural Resources, 63(3):241-255.