Effect of BAP and NAA on Micropropagation of Caladium bicolor (Aiton) Vent., an Ornamental Plant

Journal of Ornamental Plants

مقاله 7، دوره 6، شماره 1، خرداد 2016، صفحه 59-66   

نوع مقاله: Research Paper

نویسندگان

Shima Seydi¹؛ Naser Negahdar²؛ Raziye Taghizadeh Andevari³؛ Mohammad Hossein Ansari⁴؛ Behzad Kaviani^{* 1}

¹Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

²Master of Science and Ph.D. Student, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran

³Master of Science, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran

⁴Department of Agronomy, Rasht Branch, Islamic Azad University, Rasht, Iran

چکیده

Leaf explants of kaladium dwubarwne Caladium bicolor (Aiton) Vent., were cultured in vitro on MS media supplemented with 25 different concentrations of BAP and NAA in order to determine the appropriate concentrations for micropropagation. All combinations induced callus formation on explants. Callus production on leaves explants grown on control medium was very low. The medium enriched with 4 mg l^-1 BAP + 0.5 mg l^-1 NAA was the most effective for callus formation. The highest number of shoots (6.43 per explant) and roots (5.56 per explant) were regenerated on media containing 1 mg l^-1 BAP + 0.5 mg l^-1 NAA and 3 mg l^-1 BAP + 0.5 mg l^-1 NAA, respectively. The regenerated plantlets were grown in a greenhouse and acclimatized successfully.

کلیدواژه‌ها

Araceae؛ In vitro propagation؛ plant growth regulators؛ tissue culture

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