Detection of Adnovirus in children less than 5 years old with acute gastroenteritis (AGE) from Bogota, Colombia.

International Journal of Molecular and Clinical Microbiology

مقاله 6، دوره 6، شماره 2، اسفند 2016، صفحه 700-707   

نوع مقاله: Research Article

نویسندگان

Hernan Vargas^{* 1}؛ Katherine De La Rosa²؛ Deisy Blanco³؛ Leidy Pedraza³؛ Liliana Diaz⁴؛ Sandra Gómez⁵؛ Jenny Nuñez⁶؛ Diane Moyano⁶؛ Patricia Arce⁷؛ Carlos Daniel Golijow⁸

¹Biólogo PhD. Profesional Especializado Laboratorio de Salud Publica Secretaria Distrital de Salud

²Estudiante investigadora:Bacteriología y laboratorio clínico, laboratorio de salud pública, secretaría de salud de Bogotá.

³Profesional en Bacteriología y laboratorio clínico

⁴MD-Msc

⁵Microbióloga - MSc

⁶Bacterióloga y laboratorista clínico - MSc

⁷Enfermera - MSc

⁸Instituto de Genética Veterinaria “Ing. Fernando Dulout” (IGEVET, CONICET-UNLP, Argentina)

چکیده

Acute gastroenteritis (AGE) has a great impact on children less than five years of age, producing high levels of morbidity and mortality. Worldwide, AGE is mainly related to viruses, mainly rotavirus but human adenovirus (HAdV) in particular as an important etiologic agent. Among HAdV species F, genotypes 40 and 41 are responsible for a high number of diarrhea cases, mainly in children under 2 years of age. Two hundred and fifty four fecal samples were analyzed using conventional PCR to detect and characterize enteric HAdV in children less than five years diagnosed with AGE and recruited between 2012 and 2013 through the Sentinel Surveillance Program from Bogota DC, Colombia. Fourteen samples (5.51%) were positive for HAdV. Species-specific detection showed one HAdV-A sample, four HAdV-B samples, two HAdV-D samples, two HAdV-E samples, and five HAdV-F samples. HAdV-F samples were subjected to conventional PCR in order to identify genotypes 40 and 41. All HAdV-F samples were genotype 40, except for one sample coinfected with both genotypes (40 and 41). Our results show that enteric HAdV are important agents in the etiology of AGE in children less than 5 years old. Moreover, the species-specific PCR analysis demonstrated that species other than HAdV- F which are tightly associated with respiratory tract disease, could infect and produce gastrointestinal tract disorders.

کلیدواژه‌ها

Human Adenovirus؛ acute gastroenteritis؛ genotypes adenovirus؛ Children؛ PCR