Application of Magnetic Polymer Particles Modified with β–Cyclodextrin for Adsorption of Bovine Serum Albumin

Journal of Applied Chemical Research

مقاله 5، دوره 11، شماره 2، مرداد 2017، صفحه 51-64   

نویسندگان

Mahshid Nikpour Nezhati^{* 1}؛ Habibollah Baharvand²؛ Zahra Ghorbani¹

¹Department of Chemistry, Islamic Azad University Central Tehran branch, Tehran, Iran

²Department of Polymer Science, Iran Polymer & PetrochemicalInstitute,Tehran, Iran

چکیده

Magnetic polymer particles which were modified by vinyl groups and subjected to
polymerization by vinyl β-Cyclodextrin derivative, has been used as adsorbent for sorption and
release of bovine serum albumin and the equilibrium and kinetics of the adsorption process were
studied. The absorbability and releasing of this protein through the new polymer has been
measured by ultraviolet-visible spectroscopy. Effects of various parameters such as pH, contact
time and capacity for adsorption of bovine serum albumin have been studied in vitro and the
optimum magnitudes were 4, 37.94 mg/g and 5 minutes respectively. The experimental results
show that the adsorption of Bovine serum albumin on magnetic particles was affected greatly by
the pH andthe adsorption equilibrium isotherm was fitted well by the Freundlich model. Bovine
serum albumin was released from magnetic particles in simulated intestinal fluids. It was
released up to about 7 h at high speed and then it released slowly up to 30 h.

کلیدواژه‌ها

Bovine serum albumin؛ Magnetic polymer particles؛ β-cyclodextrin؛ Biocompatible؛ Biodegradable؛ hydrogel