Physiological and phytochemical changes induced by seed pretreatment with hydrogen peroxide in Artemisia sieberi under salt stress.

Iranian Journal of Plant Physiology

مقاله 1، دوره 7، شماره 1، دی 2016، صفحه 1875-1887   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2016.532427

نویسندگان

Forouzan Azimian؛ Parto Roshandel^*

Biology Department, Faculty of Sciences, Shahrekord University, Iran

چکیده

Seeds of medicinal plant Artemisia sieberi were pretreated with H₂O₂ (0, 10, 50, 90, and 140 µM) and grown in saline condition (0 and 150 mM NaCl) for one month. Phytochemical properties such as antioxidant capacity and also salt tolerance in the plans arising from H₂O₂ pretreated seeds under salt stress were examined. Results showed a decrease in H₂O₂ and malondialdehyde concentrations in the shoots of pretreated A. sieberi. Furthermore, seed pretreatment with H₂O₂ (particularly at 50 µM) increased photosynthetic pigments content, antioxidant capacity and dry and fresh weights of shoots in the pretreated plants under both normal and saline conditions. The activity of the antioxidant enzymes increased in all pretreated A. sieberi plants particularly at 50 µM H₂O₂. Data indicated H₂O₂pretreatment can induce salt tolerance in A. sieberi by supporting physiological and phytochemical processes such as photosynthesis, reactive oxygen species scavenging and detoxification, and also membrane stability in this species. Additionally, hydrogen peroxide pretreatment enhanced reducing power and antioxidant activity in A. sieberi suggesting an increase in its medicinal properties.

کلیدواژه‌ها

Artemisia sieberi؛ Antioxidant Activity؛ H2O2-priming؛ salt tolerance؛ Oxidative stress