Expression of aflR, veA and laeA as regulators of aflatoxins and cyclopiazonic acid biosynthesis pathway in Aspergillus flavus

International Journal of Molecular and Clinical Microbiology

مقاله 7، دوره 7، شماره 1، شهریور 2017، صفحه 787-794   

نوع مقاله: Research Article

نویسندگان

Saeideh Amani Ghayum¹؛ Masoomeh Shams-Ghahfarokhi^{* 1}؛ Mehdi Razzaghi-Abyaneh²

¹Department of Mycology, Faculty of Medical Sciences, TarbiatModares University, Tehran 14115-331, Iran

²Department of Mycology, Pasteur Institute of Iran, Tehran 13164, Iran

چکیده

In this study, the production of aflatoxin B1 (AFB1) and cyclopiazonic acid (CPA) was investigated in toxigenic and non-toxigenic Aspergillus flavus with respect to expression of aflR, veA and laeA genes that are involved to toxins production. A. flavus strains were cultured in YES broth at 28 °C for 4 days and the presence of (AFB1) and (CPA) was confirmed and measured by TLC and HPLC. The expression of aflR, veA and laeA was compared in toxigenic and non-toxigenic strains after cDNA preparation by Real-Time PCR. The results showed that the highest concentrations of AFB1 and CPA were 9450.56 and 403.85µg/g fungal dry weight, respectively. A. flavus isolates based on the ability for producing mycotoxins were divided into 4 groups including, AFB1 and CPA producer (9450.56 and 377.52 µg/g; chemotype I), AFB1 producer (2024.80 µg/g; chemotype II), CPA producer (403.85 µg/g; chemotype III), and non-producer (chemotype IV). The results of the analysis of aflR, veA and laeA gene expression between toxigenic and non-toxigenic A. flavus isolates did not show any significant correlation between the expression of these genes and AFB1 and CPA production among the tested strains in our study. Since, the incidence of AFB1 and CPA producing Aspergillus in environmental pollution is a potential threat to public health, finding the role of related genes of the biosynthetic pathway of aflatoxin and CPA in determining the mycotoxins producing A. flavus is recommended in a larger population for different geographic locations.

کلیدواژه‌ها

Aspergillus flavus؛ Aflatoxin B1 (AFB1)؛ Cyclopiazonic acid (CPA)؛ aflR؛ veA؛ laeA؛ Gene expression