Removal of malachite green by using immobilized glucose oxidase onto silica nanostructure-coated silver metal-foam

Journal of Nanoanalysis

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 04 مهر 1396

نوع مقاله: Original Research Paper

شناسه دیجیتال (DOI): 10.22034/jna.2017.538858.1002

نویسندگان

Mansour Hemmati¹؛ Mohammad Barkhi²؛ Hadi Baharifar³؛ Kamyar Khoshnevisan^{* 4}

¹Nano Pooshesh Felez Co., University of Tehran science and Technology Park, Tehran, Iran

²University of Applied Science and Technology (UAST), Zar Center, Karaj, Iran

³Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

⁴Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

چکیده

Enzymes Immobilization onto different types of substrate could be helpful in various applications of biomedical devices and biosensors. Enzyme activity and stability could be affected by support and method of immobilization. In this study, the silver metal foam was successfully synthesized by soft-shell method and then was coated with silica. Then, glucose oxidase (GOx) was immobilized on non-coated and silica-coated silver metal foam and removal of malachite green was investigated. Fourier Transform Infrared Spectroscopy (FT-IR) and scanning electron microscopy (SEM) results confirmed that the enzyme was attached to support surface. The maximum immobilized enzyme activity was about 118.980 U/grSupport at 40ᵒC. The removal of malachite green showed the indirect relation with dye concentration confirmed by decolorization assay. The increasing activity, thermal stability, and reusability of immobilized glucose oxidase onto the silver metal foam in comparison to free enzyme introduce the capability of the biological system for removal of malachite green in the industry.

کلیدواژه‌ها

Enzyme immobilization؛ Glucose oxidase؛ Silver metal foam؛ Enzyme activity؛ Malachite green